全文获取类型
收费全文 | 71篇 |
免费 | 5篇 |
国内免费 | 4篇 |
专业分类
化学 | 55篇 |
力学 | 4篇 |
数学 | 9篇 |
物理学 | 12篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 6篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 9篇 |
2012年 | 6篇 |
2011年 | 2篇 |
2010年 | 2篇 |
2009年 | 3篇 |
2008年 | 2篇 |
2007年 | 3篇 |
2006年 | 3篇 |
2005年 | 2篇 |
2004年 | 1篇 |
2003年 | 2篇 |
1999年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1988年 | 1篇 |
1984年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有80条查询结果,搜索用时 15 毫秒
1.
Three new serratanes were isolated from the nonsaponifiable fraction of western white pine ( Dougl.) bark. The compounds were shown to be 3β-methoxyserrat-14-ene-21α,30-diol (), 3β-methoxyserrat-14-ene-21α, 29-diol (), and 3β-methoxyserrat-14-ene-21β,30-diol (), by a combination of chemical, and spectral methods. 相似文献
2.
3.
Direct Blue 71 staining as a destaining‐free alternative loading control method for Western blotting
Li Zeng Jing Guo Hong‐Bo Xu Rongzhong Huang Weihua Shao Liu Yang Mingju Wang Jianjun Chen Peng Xie 《Electrophoresis》2013,34(15):2234-2239
In Western blotting, a suitable loading control is indispensable for correcting errors in the total amount of loaded protein. Immunodetection of housekeeping proteins and total protein staining have traditionally been used as loading control methods. Direct Blue 71 (DB71) staining—a novel, sensitive, dye‐binding staining method compatible with immunodetection—may offer advantages over these traditional loading control methods. Three common neuroscientific samples (human plasma, human oligodendrocytes, and rat brain) were employed to assess DB71 staining as a loading control method for Western blotting. DB71, CBB, one traditional housekeeping protein, and one protein of interest were comparatively assessed for reliability and repeatability and linear dynamic range over 2.5–40 μg of protein loaded. DB71's effect on the reliability and repeatability and linear dynamic range of immunoreaction were also assessed. Across all three sample types, DB71 was either equivalent or superior to CBB and housekeeping protein‐based methods in terms of reliability and repeatability and linear dynamic range. Across all three sample types, DB71 staining did not impair the reliability and repeatability or linear dynamic range of immunoreaction. Our results demonstrate that the DB71 staining can be used as a destaining‐free alternative loading control method for Western blotting. 相似文献
4.
András Kovács Zoltán Patai András Guttman János Kádas László Takács István Kurucz 《Electrophoresis》2013,34(20-21):3064-3071
Immunization with complex mixtures, like the human plasma resulted in the generation of cloned mAb libraries (PlasmaScan? and QuantiPlasma? libraries, with >1000 individual mAbs) reacting with a nonredundant set of antigenic epitopes. mAb proteomics refers to quasi‐hypothesis‐free profiling of plasma samples with nascent or cloned mAb libraries for the discovery of disease‐specific biomarkers. Once mAbs with biomarker potential have been identified, the next task is the determination of cognate antigens recognized by the respective mAbs. To determine the cognate protein antigen corresponding to each individual mAbs in the cloned mAb libraries, we have separated human plasma by consecutive steps of desalting and various chromatography procedures. The process resulted in 783 fractions, which we termed “Analyte Library” (AL). The AL represents the human plasma proteome in relatively low‐protein complexity fractions. Here, to determine the utility of the AL, we selected ten plasma proteins and checked for their presence in the fractions. Among the ten cases, the distribution of four selected plasma proteins matched expectations, as these proteins were present only in a few fractions corresponding to their physical, chemical, and biochemical properties. However, in six cases, we observed “smear” ‐like distribution or complete absence of the proteins, suggesting that protein–protein interactions or protein variants may alter the observed plasma distribution profiles. Nevertheless, we conclude that the AL is an efficient, high throughput tool to complement the mAb biomarker discovery process with cognate protein antigen identification for each mAbs. 相似文献
5.
Zhen Wang Ziyang Li Tian Su Xiao Han Zhanwu Hou Yupeng Zheng Jiachen Liu Jun Xu Jeffy Yang Huadong Liu 《Electrophoresis》2021,42(6):793-799
Western blot (protein immunoblot) is a widely used analytical technique in molecular biology. Utilizing the specific recognizing primary antibody, proteins immobilized on various matrix are investigated by subsequent visualization steps, for example, by the horse radish peroxidase conjugated secondary antibody incubation. Methods to improve the sensitivity in protein identification or quantification are appreciated by biochemists. Herein, we report a new strategy to amplify Western blot signals by constructing a probe with proximal labeling and IgG targeting abilities. The R118G mutation attenuated the biotin-AMP binding affinity of the bacterial biotin ligase BirA*, offering a proximity-dependent labeling ability, which could be used as a signal amplifier. We built a BirA*-protein A fusion protein (BioEnhancer) that specifically binds to IgG and adds biotin tags to its proximal amine groups, enhancing the immunosignal of target proteins. In our experiments, the BioEnhancer system amplified the immunosignal by tenfold compared to the standard western blot. Additionally, our strategy could couple with other signal enhancement methods to further increase the western blot sensitivity. 相似文献
6.
在古代中国,“力”字和“力学”一词早就出现了。“力”的含义最初是对“人的力量”的直观描述,后来发展到对一般物体的重力的描述,再到对运动状态原因的描述。本文对“力”字含义的变迁进行了辨析。中国现代“力学”是西学东渐的结果,学科名字的确定过程也有不小的 曲折。通过分析确定“力学”为学科名字的过程,揭示了中国古人对“力学”学科理解的变迁。 相似文献
7.
Southwestern blotting is when a DNA sequence is used to probe DNA-binding proteins on an electrophoretic gel blot. It would be highly desirable to be able to probe a blot repeatedly with different DNA sequences. Alkaline phosphatase can remove 5'-phosphoryl groups from DNA and radiolabeled 5'-(32)P-DNA probes are commonly used in Southwestern blotting. Here is shown that once probed, the radioisotope signal on the blot can be effectively removed by brief digestion with alkaline phosphatase, and the blot can then be repeatedly probed at least six times with different DNA probes. This exceeds the repetitions possible with another commonly used method using SDS. The technique can be used with either one-dimensional or multi-dimensional Southwestern blots and does not have a large effect on the phosphorylation state of the blotted proteins. An alternative method using T4 polynucleotide kinase stripping is also introduced but was less well characterized. 相似文献
8.
Effects of South China Sea/western North Pacific summer monsoon on tropospheric biennial oscillation (TBO) 总被引:2,自引:0,他引:2 下载免费PDF全文
Several theories have been developed to explain tropical biennial
oscillation (TBO), as an air--sea interactive system to impact Asian
and global weather and climate,
and some models have been established to produce a TBO. A
simple 5-box model, with almost all the key processes associated with
TBO, can produce a TBO by including air--sea interactions in the
monsoon regions. Despite that, the South China Sea/western North
Pacific summer monsoon (SCS/WNPSM), a very important monsoon
subsystem, is neglected. In this paper, based on the dynamical
framework of 5-box model, the term of SCS/WNPSM has been added and a
6-box model has been developed. Comparing the difference of TBO
sensibilities with several key parameters, air--sea coupling
coefficient α, SST-thermocline feedback coefficient
γ and wind-evaporation feedback coefficient λ,
between the modified model and original model, TBO is more sensible
to the parameters in the new model. The results imply that the
eastern Pacific and local wind-evaporation play more important roles
in the TBO when including SCS/WNPSM. 相似文献
9.
Hydatid cyst fluids (HCF) crude extracts from camels and sheep slaughtered in Riyadh region, KSA were subjected to Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS–PAGE) and Western blot analysis. Sera from 17 confirmed human cases of hydatidosis, 25 patients with other parasitic infections and 10 clinically healthy subjects were used to evaluate the diagnostic value of the different antigenic fractions of these extracts. Immunoblotting results revealed that, at least 11 major discrete protein fractions (110–8 kDa) were recognized by sera from hydatidosis patients, sera from patients with other parasitic diseases showed cross-reactivity with few of these bands. The cluster of bands (38–35 kDa) that may be a breakdown of “Arc 5” antigen (39–38 kDa) was detected by 100% and 94% of sera from hydatidosis cases with HCF extracts from camel and sheep, respectively. This cluster showed also some cross reactivity (20% and 8%) with control sera from patients with other parasitic infections with camel and sheep HCF extracts, respectively. Polypeptides at 24–22, 16 and 8 kDa which may probably correspond to antigen B subunits were also identified by all samples from hydatidosis patients with sheep HCF extracts and by 100%, 65% and 74% with camel HCF extracts respectively. Sera from control subjects did not react with any of these polypeptides (24–22, 16 and 8 kDa). According to our results, the identified molecular weight bands (16 and 8 kDa using HCF crude extracts from sheep and 24–22 kDa using HCF crude extracts either from camel or sheep) represent good candidates for immunodiagnosis of hydatidosis. 相似文献
10.
黄霖 《广东微量元素科学》1995,2(11):45-48
用参麦汤合葡萄糖酸锌片治疗复发性口疮102例,经过3个月治疗,观察到该药能有效地改善临床症状,降低复发率,明显地提高体内锌的水平,与维生素类对照组比较,疗效有显著性差异(P<0.01)。 相似文献