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排序方式: 共有1462条查询结果,搜索用时 31 毫秒
1.
Youchao Wang Na Tian Weize Sun Boerhan Rena Xusheng Guo Yang Feng Chao Li Xuesong Wang Qianxiong Zhou 《Particle & Particle Systems Characterization》2020,37(5):2000045
Photoactivated chemotherapy (PACT) has appealing merits over traditional chemotherapy as well as photodynamic therapy (PDT) by virtue of its spatial and temporal control on drug activity and oxygen-independent mechanisms of action. However, the short photoactivation wavelengths, e.g., visible light–activated Ru(II)-based PACT agents, limit the clinical application severely. In this work, a facile construction of supramolecular nanoparticles from a poly(ethylene glycol) (PEG)-modified [Ru(dip)2(py-SO3)]+ (abbreviated as Ru-PEG, dip = 4,7-diphenyl-1,10-phenanthroline, py-SO3 = pyridine-2-sulfonate) and 1,3-phenylenebis(pyren-1-ylmethanone) (BP) is shown. While Ru-PEG may undergo photoinduced ligand dissociation and release anticancer species of [Ru(dip)2(H2O)2]2+, BP has extremely large two-photon absorption cross sections (δ2) in the NIR region and intense fluorescence over the wavelengths where Ru-PEG has strong absorption. Thus, two-photon excitation of BP followed by an efficient Förster resonance energy transfer (FRET) from BP to Ru-PEG may lead to a potent inactivation against cisplatin-resistant cancer cells and 3D multicellular tumor spheroids (MCTSs). The residue fluorescence of BP also allows the cellular uptake of the particles to be visualized. This work provides a universal and convenient strategy to realize theranostic PACT in the ideal phototherapeutic window of 650–900 nm. 相似文献
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Dr. Jun Zeng Dr. Zhichao Wang Dr. Xin Huang Dr. Sabine S. Eckstein Prof. Dr. Xiaohui Lin Prof. Dr. Hailong Piao Prof. Dr. Cora Weigert Dr. Peiyuan Yin Prof. Dr. Rainer Lehmann Prof. Dr. Guowang Xu 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(21):5427-5432
Mass spectrometry (MS) driven metabolomics is a frequently used tool in various areas of life sciences; however, the analysis of polar metabolites is less commonly included. In general, metabolomic analyses lead to the detection of the total amount of all covered metabolites. This is currently a major limitation with respect to metabolites showing high turnover rates, but no changes in their concentration. Such metabolites and pathways could be crucial metabolic nodes (e.g., potential drug targets in cancer metabolism). A stable-isotope tracing capillary electrophoresis–mass spectrometry (CE-MS) metabolomic approach was developed to cover both polar metabolites and isotopologues in a non-targeted way. An in-house developed software enables high throughput processing of complex multidimensional data. The practicability is demonstrated analyzing [U-13C]-glucose exposed prostate cancer and non-cancer cells. This CE-MS-driven analytical strategy complements polar metabolite profiles through isotopologue labeling patterns, thereby improving not only the metabolomic coverage, but also the understanding of metabolism. 相似文献
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光照和氮素对外来植物凤眼莲生长和生理特性的影响 总被引:1,自引:0,他引:1
通过研究不同光照和氮素营养处理的外来植物凤眼莲的生长、生物量分配、硝酸还原酶活性、游离氨基酸以及可溶性蛋白质含量变化,探讨其对环境适应性的生理学机制。凤眼莲表现出极强的可塑性,随光照和氮素营养的增加,凤眼莲生长速率明显加快,氮素代谢关键酶硝酸还原酶活性上升。根部吸收的硝酸根离子大部分运输到叶片中还原,氮素同化效率高。氨基酸含量和可溶性蛋白质含量呈现明显的变化,叶片可溶性蛋白质含量与根冠生物量分配显著相关。本研究表明风眼莲对光照和氮素表现出很强的适应性,其快速生长和高可塑性依赖于对环境变化的牛理响廊。 相似文献
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Hiroshi Yamauchi Keiko Takahashi Mari Mashiko Juichiro Saitoh Yukio Yamamura 《应用有机金属化学》1992,6(4):383-388
We calculated the intake of each chemical species of dietary arsenic by typical Japanese, and determined urinary and blood levels of each chemical species of arsenic. The mean total arsenic intake by 35 volunteers was 195±235 (15.8-1039) μg As day?1, composed of 76% trimethylated arsenic (TMA), 17.3% inorganic arsenic (Asi), 5.8% dimethylated arsenic (DMA), and 0.8% monomethylated arsenic (MA): the intake of TMA was the largest of all the measured species. Intake of Asi characteristically and invariably occurred in each meal. Of the intake of Asi, 45-75% was methylated in vivo to form MA and DMA, and excreted in these forms into urine. The mean measured urinary total arsenic level in 56 healthy volunteers was 129±92.0 μg As dm?3, composed of 64.6% TMA, 26.7% DMA, 6.7% Asi and 2.2% MA. The mean blood total arsenic level in the 56 volunteers was 0.73±0.57 μg dl?1, composed of 73% TMA, 14% DMA and 9.6% Asi. The urinary TMA levels proved to be significantly correlated with the whole-blood TMA levels (r = 0.376; P<0.01). 相似文献
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The toxicity of inorganic trivalent arsenic for living organisms is reduced by in vivo methylation of the element. In man, this biotransformation leads to the synthesis of monomethylarsonic (MMA) and dimethylarsinic (DMA) acids, which are efficiently eliminated in urine along with the unchanged form (Asi). In order to document the methylation process in humans, the kinetics of Asi, MMA and DMA elimination were studied in volunteers given a single dose of one of these three arsenicals or repeated doses of Asi. The arsenic methylation efficiency was also assessed in subjects acutely intoxicated with arsenic trioxide (As2O3) and in patients with liver diseases. Several observations in humans can be explained by the properties of the enzymic systems involved in the methylation process which we have characterized in vitro and in vivo in rats as follows: (1) production of Asi metabolites is catalyzed by an enzymic system whose activity is highest in liver cytosol; (2) different enzymic activities, using the same methyl group donor (S-adenosylmethionine), lead to the production of mono- and di-methylated derivatives which are excreted in urine as MMA and DMA; (3) dimethylating activity is highly sensitive to inhibition by excess of inorganic arsenic; (4) reduced glutathione concentration in liver moderates the arsenic methylation process through several mechanisms, e.g. stimulation of the first methylation reaction leading to MMA, facilitation of Asi uptake by hepatocytes, stimulation of the biliary excretion of the element, reduction of pentavalent forms before methylation, and protection of a reducing environment in the cells necessary to maintain the activity of the enzymic systems. 相似文献
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Ginette Ratovo Jean‐Pierre Souchard Pascale Urizzi Yvon Coulais Franoise Nepveu Etienne Hollande 《应用有机金属化学》2004,18(1):1-8
Pancreatic cancer has an extremely poor prognosis, due, in part, to lack of methods for early diagnosis. The present study was designed to evaluate the potential of labeling low‐density lipoprotein (LDL) with a radionuclide using a lipid chelating agent, bis(stearylamide) of diethylenetriaminepentaacetic acid (L), to detect pancreatic tumors by gamma‐scintigraphy. Previous studies indicated that the difficulty of visualization of pancreatic tumors was due to their poor vascularization. This study compares the ability of two radiotracers, 111In–L–LDL and 153Gd–L–LDL to target highly vascularized rat pancreatic tumors (AR4‐2J) implanted in nude mice. Biodistribution studies showed that the tumor uptake of 111In–L–LDL and 153Gd–L–LDL tracers was twofold and fivefold higher respectively than with the controls (111In citrate and 153Gd citrate respectively). These tracers would thus be suitable for scintigraphic imaging. We show here that LDL could be employed as a delivery system for tracers such as 111In or 153Gd when these two radionuclides are complexed by a lipid‐chelating anchor, and that 111In–L–LDL and 153Gd–L–LDL enabled better visualization of the pancreatic tumor tissues, with a better result with 153Gd–L–LDL. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献