HPLC法测定二(2-噻吩基)乙醇酸东莨菪酯和2,2-二噻吩基乙醇酸甲酯的含量

建立了测定二(2-噻吩基)乙醇酸东莨菪酯和2,2-二噻吩基乙醇酸甲酯的含量的HPLC方法。采用μBondapakTMC18液相色谱柱(3.9mm i.d.×250mm,10μm),以乙腈/戊烷磺酸钠溶液(35/65,v/v,pH=7.0)为流动相,流速设定为0.8mL.min-1,检测波长为215nm时,实现了这两种酯的良好分离分析。二(2     

Highly porous poly(lactide‐co‐glycolide) microparticles for sustained tiotropium release

In this study, porous poly(lactide‐co‐glycolide) (PLGA) microparticles with low mass density and large particle size were developed for chronic obstructive pulmonary disease treatment using anticholinergic drug (tiotropium). The porous PLGA microparticles were prepared by the water‐in‐oil‐in‐water (W₁/O/W₂) multi‐emulsion method using PLGA polymer and ammonium bicarbonate (as a porogen). Herein, soluble starch was incorporated in porous PLGA microparticles for long‐term tiotropium release. In vitro drug release studies determined that the rapid release of tiotropium from porous PLGA microparticles was reduced because of the high viscosity of the incorporated starch. Tiotropium release from porous PLGA microparticles continued up to 3 days. Furthermore, the inhaled microparticles showed longer drug residence in in vivo lung epithelium. Copyright © 2013 John Wiley & Sons, Ltd.