毛细管电泳在拮抗药物对氨基苯甲酸、对羟基苯甲酸和磺胺分析测定中的应用

本文报道了一种用毛细管区带电泳法(CZE)分离与测定对氨基苯甲酸、对羟基苯甲酸及磺胺类药物的新方法.电泳条件为:用 20mmol/L硼砂-20mmol/L H_3PO_4-20 mmol/Lβ-环糊精-4％乙醇(pH 7.0)作电泳液,L-抗坏血酸为内标,280nm为检测波长,样品由电进样方式(10kV/10s)引入毛细管(51.2 cm×50μm i.d.,有效分离长度为 38.5 cm).在24.5°C下,6 min内三者可达基线分离(电泳电压 25kV),且在一定范围内可进行定量分析,保留时间(Tr)及A_(样品)/A_(内标)的RSD值分别小于1.0％和5.0％.本法的建立为研究这三者共存于高等动物及微生物体内时的生理作用提供了一种可共选择的新方法.     

Novel Sulfanilamide as Potent Surfactants and Antibacterial Agents

Sulfanilamide derivatives of the type RØSO₂NHR in which substitution at both the amino and amido nitrogen have been synthesized where R = NHCOCH:CHCOOH, NHCOCH₂CH₂COOH, NHCH₂CH(OH)CH₂SO₃Na, N⁺(CH₃)₃I^? and NHCH₂CH(OH)CH₂N⁺(C₂H₅)₃Cl^? and R′ = butyl, cyclohexyl, and decyl. The unique structural features of these sulfanilamides were confirmed by infrared, proton magnetic resonance and elemental analysis. Most of these compounds exhibited good water solubility and surface activity. Wetting ability and tendency of enhancing oil/water emulsification were related to the structure of the substituents in sulfanilamide molecule. Furthermore, the peculiar of molecular structure of these sulfanilamide surfactants imparts distinguished antimicrobial activity to these molecular species against some Gram positive and Gram negative bacteria.     

An overview of analytical methodologies for the determination of antibiotics in environmental waters

The widespread occurrence of antibiotics as contaminants in the aquatic environment has increased attention in the last years. The concern over the release of antibiotics into the environment is related primarily to the potential for the development of antimicrobial resistance among microorganisms. This article presents an overview of analytical methodologies for the determination of quinolone (Qs) and fluoroquinolone (FQs), macrolide (MLs), tetracycline (TCs), sulfonamide (SAs) antibiotics and trimethoprim (TMP) in different environmental waters. The analysis of these antibiotics has usually been carried out by high-performance liquid chromatography (HPLC) coupled to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) and to a lesser extent by ultraviolet (UV) or fluorescence detection (FD). A very important step before LC analysis is sample preparation and extraction leading to elimination of interferences and prevention of matrix effect and preconcentration of target analytes.     

Determination of mimosine by a sensitive indirect spectrophotometric method

A simple and sensitive indirect spectrophotometric method is described for the determination of mimosine based on its reaction with diazotized sulfanilamide (DZSAM). DZSAM couples with N-(1-naphthyl)ethylenediamine (NEDA) forming a pink colored azodye, absorbing maximally at 540 nm (ε_max=27 mM⁻¹ cm⁻¹). In the present method, mimosine was first reacted with known excess of DZSAM and the unreacted DZSAM was determined by coupling with NEDA. The reaction of mimosine with DZSAM proceeded optimally at neutral pH. The decrease in absorbance of the DZSAM-NEDA-coupled product obeyed Beer’s law in the concentration range of 0.005-0.15 μg ml⁻¹ of mimosine. The present method was applied to estimate mimosine in plant extracts containing lesser than 0.05 μg ml⁻¹ with recovery at 99±0.41%. The method described is superior to other reported methods in terms of ease of adaptability and sensitivity.     

Surface‐enhanced Raman scattering of benzenesulfonamide and sulfanilamide adsorbed on silver nanoparticles

The Surface‐enhanced Raman scattering of benzenesulfonamide and sulfanilamide adsorbed on silver sols was studied. On the basis of the noticeable shifts observed for wavenumbers of the ν_s(OSO), ν(CS), and ν(SN) vibrations with respect to the Raman spectra of the solids and the ionic solutions, we conclude that these molecules are adsorbed on silver nanoclusters at pH ≥ 7 with the aminosulfonyl groups partially deprotonated. The benzenesulfonamide links to the metal through the nitrogen atom of the corresponding azanion, while the sulfanilamide interacts in turn through the nitrogen atoms of the –NH₂ and –SO₂NH^– groups in the para‐position. Additionally, it was found that the most enhanced surface‐enhanced Raman scattering bands, especially the 8a;ν_ring mode, are related to the presence of the charge transfer mechanism. Copyright © 2011 John Wiley & Sons, Ltd.     

Synthesis, Crystal Structure and Fluorescent Property of a New Sulfanilamide Schiff-base Compound

A new sulfanilamide Schiff-base compound N,N'-(p-phenyl sulfanilamide)-5,6-dimethoxy-pyrimidine-(2-hydroxyl benzene) methylidene has been synthesized by the condensation of equimolar salicylaldehyde and 4-(p-amino phenyl sulfanilamide)-5,6-dimethoxy-pyrimidine in an anhydrous ethanol solution.The compound was characterized by elemental analysis,IR spectra,and single-crystal X-ray diffraction.The crystal belongs to the monoclinic system,space group P21/c with a=19.0425(11),b=11.1914(6),c=9.0075(5),β=93.9650(10)°,Z=4,V=1915.01(18)3,Dc=1.437 g/cm3,Mr=414.43,λ(MoKa)=0.71073,μ=2.09 mm-1,F(000)=864,R=0.0385 and wR=0.1224.     

1－芳酰基－3－（4－苯磺酰胺）硫脲类化合物的合成

以无水丙酮作溶剂，由芳酞基异硫氰酸酯与磺胺反应合成了一系列薪的１－芳酸基－３－（４－苯磺酰胺）硫脲。     

磺胺多金属氧酸盐对人宫颈癌Hela细胞的抑制作用

对磺胺多金属氧酸盐（C6H9N2O2S）5HP2Mo18O62·15H2O（SPOM-1）,（C6H9N2O2S）H8P2Mo15V3O62·8H2O（SPOM-2）进行了体外毒性实验和抑制宫颈癌Hela细胞作用的研究．当SPOM浓度为50—100mg·L^-1时,对人胚胎成纤维细胞均无细胞毒性作用（P〉0．05）,TC50分别为459mg·L^-1、295mg·L^-1;抑制宫颈癌He—la细胞的EC50分别为88mg·L^-1、24mg·L^-1,治疗指数即分别为5．22,12．29．     

二苯基哌嗪磺胺化合物的合成及其抗微生物活性

以乙酰苯胺和4-氯二苯甲酮为起始原料,经多步反应方便而有效地合成了二苯基哌嗪磺胺化合物,所得目标化合物及其中间体的结构经1H NMR、13C NMR、IR、MS谱和元素分析等证实.研究了化合物的体外抗微生物活性,结果表明目标化合物14和16对所测菌株的抗菌活性均优于磺胺.     

Periodic mesoporous organosilicas with co-existence of diurea and sulfanilamide as an effective drug delivery carrier

In this article we report the synthesis of new periodic mesoporous organosilicas (PMOs) with the co-existence of diurea and sulfanilamide-bridged organosilica that are potentially useful for controlled drug release system. The materials possess hexagonal pores with a high degree of uniformity and show long-range order as confirmed by the measurements of small-angle X-ray scattering (SAXS), N₂ adsorption isotherms, and transmission electron microscopy(TEM). FT-IR and solid state ²⁹Si MAS and ¹³C CP MAS NMR spectroscopic analyses proved that the bridging groups in the framework are not cleaved and covalently attached in the walls of the PMOs. It was found that the organic functionality could be introduced in a maximum of 10 mol% with respect to the total silicon content and be thermally stable up to 230 °C. The synthesized materials were shown to be particularly suitable for adsorption and desorption of hydrophilic/hydrophobic drugs from a phosphate buffer solution at pH 7.4.