排序方式: 共有7条查询结果,搜索用时 62 毫秒
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中性红作试剂荧光光度法测定亚硝酸根 总被引:3,自引:1,他引:3
研究了在盐酸介质中NO^-2与中性红的亚硝化反应。结果表明,在0.048mol/L的盐酸介质中,NO^-2与中性红反应生成无荧光的物质,NO^-2在40-240μg/L,范围内与荧光猝灭程度成正比,常见的共存离子不干扰其测定。所拟方法用于水样中NO^-2的测定,结果满意。 相似文献
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荧光光谱法研究罗丹明B与DNA的相互作用 总被引:1,自引:0,他引:1
在pH=7.4的三羟甲基胺基甲烷-盐酸(Tris-HCl)的缓冲溶液中,利用荧光分光光度法研究了小分子染料罗丹明B(RB)与小牛胸腺DNA(CTDNA)的相互作用.实验考察了离子强度、阴离子猝灭剂KI对RB-DNA体系的影响.结果表明:DNA的存在使得RB的荧光强度显著降低,RB以沟槽模式与DNA相互作用. 相似文献
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痕量草酸的催化荧光分析 总被引:1,自引:0,他引:1
在0.04mol/L H2SO4介质中及80℃条件下,草酸对K2Cr2O7氧化罗丹明B这一荧光猝灭反应具有较强的催化作用。以自来水冷却中止反应,采用荧光法研究了催化测定草酸的各种影响因素,建立了一个测定范围和检出限分别为0.03-3μg/mL和0.01μg/mL草酸的催化荧光分析法,用于尿样分析,结果满意。 相似文献
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James D. Macneil R. W. Frei O. Hutzinger 《International journal of environmental analytical chemistry》2013,93(3):205-220
The formation of π-complexes to detect and identify a representative group of carbamate, anilide and urea pesticides was investigated. Results obtained with several electron-acceptor reagents used by previous workers are compared with those obtained with a group of nitro-substituted fluorene derivatives. The pesticides studied were chosen for their potential ability to act as electron donors. Detection limits after thin-layer chromatography ranged from 0.5 to 5.0 micrograms, depending upon the strength of the complex formed. Various chromatographic systems were studied and applications of in situ reflectance spectroscopy and mass spectrometry were investigated. The method combines a reasonable sensitivity with good selectivity and the pesticide may be recovered from the complex for further study. This non-destructive method of analysis should be of particular use in the separation and identification of these pesticides and their metabolites. 相似文献
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