Pre‐Incubation of Auric Acid with DNA Is Unnecessary for the Formation of DNA‐Templated Gold Nanoclusters

The rationale for the preparation of DNA‐templated gold nanoclusters (DNA‐Au NCs) has not been well understood, thereby slowing down the advancement of the synthesis and applications of DNA‐Au NCs. The interaction between metal ions and the DNA template seems to be the key factor for the successful preparation of DNA‐templated metal nanoclusters. With the help of circular dichroism in this contribution, we put efforts into interrogating the necessity of pre‐incubation of HAuCl₄ with poly‐adenine template in the formation of Au NCs by citrate reduction. Our results revealed that the pre‐incubation of HAuCl₄ with poly‐adenine is not favorable for the formation of Au NCs, which is distinctly different from the formation process for silver nanoclusters. It is our hope that this study can provide guidance in the preparation of Au NCs with more DNA templates.