排序方式: 共有1条查询结果,搜索用时 0 毫秒
1
1.
Ryu Hashimoto Dr. Masafumi Minoshima Dr. Junichi Kikuta Shinya Yari Prof. Steven D. Bull Prof. Masaru Ishii Prof. Kazuya Kikuchi 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(47):21182-21186
A rationally designed pH-activatable fluorescent probe (pHocas-RIS) has been used to measure localised pH levels in osteocytic lacunae in bone tissue. Conjugation of the moderate bone-binding drug risedronate to a pH-activatable BODIPY fluorophore enables the probe to penetrate osteocytic lacunae cavities that are embedded deep within the bone matrix. After injection of pHocas-RIS, any osteocytic lacunae caused by bone-resorbing osteocytes cause the probe to fluoresce in vivo, thus allowing imaging by intravital two-photon excitation microscopy. This pH responsive probe enabled the visualization of the bone mineralizing activities of acid producing osteocytes in real time, thus allowing the study of their central role in remodeling the bone-matrix in healthy and disease states. 相似文献
1