Highly selective molecular recognition and high throughput detection of melamine based on molecularly imprinted sol-gel film

Multimode reader has been generally applied in immunoassay, and in the proposed paper, the 96 well micro-plate was modified with molecularly imprinted melamine sol-gel film, based on which the highly selective and high throughput detection of melamine was achieved. Melamine was imprinted into silica sol-gel films directly using phenyltrimethoxysilane and methyltrimethoxysilane as functionalized organosilicon precursors. The binding characteristic of the imprinted film to melamine was evaluated by equilibrium binding experiments and the morphology was studied by scanning electronic microscope (SEM). Scatchard analysis was carried out to estimate the binding parameters of the imprinted film. The proposed method exhibited excellent selectivity because of specific recognition of MM by molecularly imprinted film. Under the optimum conditions, the chemiluminescence (CL) intensity had a linear relationship against the concentration of melamine over the range of 0.1-50 μg mL⁻¹ with a lower detection limit of 0.02 μg mL⁻¹.     

ON THE STABILITY OF A FUNCTIONALLY GRADED RECTANGULAR MICRO-PLATE SUBJECTED TO HYDROSTATIC AND NONLINEAR ELECTROSTATIC PRESSURES

This article studies the stability of a functionally graded clamped-clamped micro-plate subjected to hydrostatic and electrostatic pressures. Equilibrium positions of the micro-plate are determined and shown in the state control space. To study the stability of the equilibrium positions, the motion trajectories are given for different initial conditions in the phase plane. Effects of the electrostatic and hydrostatic pressure changes on the deflection and stability of the micro-plate for some sample value of k are studied and values of the applied voltage and hydrostatic pressure leading system to unstable conditions by undergoing a saddle node and homoclinic bifurcations are determined.     

基于Cosserat理论的四边简支自由振动微平板尺度效应研究

微观下材料内部结构将极大地影响材料的力学性能,对微纳米器件中典型的微平板结构尺度效应进行研究具有十分重要的意义.论文基于Cosserat理论推导出了微平板自由振动的微分方程,并根据四边简支边界条件假设振型函数,给出了固有频率的计算公式,对不同尺寸微平板固有频率的尺度效应进行了仿真分析.结果表明,考虑了尺度效应的微平板自由振动固有频率要高于经典理论中的固有频率.当特征长度与微平板厚度大小相当时,微平板固有频率表现出明显的尺度效应,并随着特征长度的增加而增大.同时,自由振动的尺度效应将随着微平板厚度的减小而逐渐增强,振动模态及长宽比不影响尺度效应.论文的研究将为微结构与系统的应用提供一定的理论基础.     

Size-dependent effect on biaxial and shear nonlinear buckling analysis of nonlocal isotropic and orthotropic micro-plate based on surface stress and modified couple stress theories using differential quadrature method

The size-dependent effect on the biaxial and shear nonlinear buckling analysis of an isotropic and orthotropic micro-plate based on the surface stress,the modified couple stress theory(MCST),and the nonlocal elasticity theories using the differential quadrature method(DQM)is presented.Main advantages of the MCST over the classical theory(CT)are the inclusion of the asymmetric couple stress tensor and the consideration of only one material length scale parameter.Based on the nonlinear von K′arm′an assumption,the governing equations of equilibrium for the micro-classical plate considering midplane displacements are derived based on the minimum principle of potential energy.Using the DQM,the biaxial and shear critical buckling loads of the micro-plate for various boundary conditions are obtained.Accuracy of the obtained results is validated by comparing the solutions with those reported in the literature.A parametric study is conducted to show the effects of the aspect ratio,the side-to-thickness ratio,Eringen’s nonlocal parameter,the material length scale parameter,Young’s modulus of the surface layer,the surface residual stress,the polymer matrix coefficients,and various boundary conditions on the dimensionless uniaxial,biaxial,and shear critical buckling loads.The results indicate that the critical buckling loads are strongly sensitive to Eringen’s nonlocal parameter,the material length scale parameter,and the surface residual stress effects,while the effect of Young’s modulus of the surface layer on the critical buckling load is negligible.Also,considering the size dependent effect causes the increase in the stiffness of the orthotropic micro-plate.The results show that the critical biaxial buckling load increases with an increase in G12/E2and vice versa for E1/E2.It is shown that the nonlinear biaxial buckling ratio decreases as the aspect ratio increases and vice versa for the buckling amplitude.Because of the most lightweight micro-composite materials with high strength/weight and stiffness/weight ratios,it is anticipated that the results of the present work are useful in experimental characterization of the mechanical properties of micro-composite plates in the aircraft industry and other engineering applications.     

Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was c...     

Nonlinear vibration analysis of laminated composite Mindlin micro/nano-plates resting on orthotropic Pasternak medium using DQM

The nonlocal nonlinear vibration analysis of embedded laminated microplates resting on an elastic matrix as an orthotropic Pasternak medium is investigated. The small size effects of micro/nano-plate are considered based on the Eringen nonlocal theory. Based on the orthotropic Mindlin plate theory along with the von Kármán geometric nonlinearity and Hamilton's principle, the governing equations are derived. The differential quadrature method (DQM) is applied for obtaining the nonlinear frequency of system. The effects of different parameters such as nonlocal parameters, elastic media, aspect ratios, and boundary conditions are considered on the nonlinear vibration of the micro-plate. Results show that considering elastic medium increases the nonlinear frequency of system. Furthermore, the effect of boundary conditions becomes lower at higher nonlocal parameters.     

板式磁颗粒化学发光免疫分析测定人血清中糖类抗原125

在传统的板式化学发光免疫分析法和管式磁颗粒化学发光免疫分析法基础上,建立了人血清中糖类抗原125(CA125)的板式磁颗粒化学发光免疫分析方法.该方法以磁性微粒子作为分离固相,96孔板为反应容器,辣根过氧化物酶(HRP)催化H2O2-luminol化学发光体系作为检测体系.本法测定CA125的检测灵敏度可达2.0U/mL,线性范围为0～400U/mL.与常用的包被板化学发光免疫分析方法对比,该方法检测范围宽.与管式磁颗粒化化学发光法比较,其分析灵敏度与精密度高、线性范围、分析通量以及分析成本方面均显示了很好的优越性.采用该方法对人血清中CA125进行测定并与罗氏全自动电化学发光系统的测值结果进行了比对,两者显示了良好的相关性.     

MEMS系统中微平板结构声振耦合性能研究

微机电系统(micro-electro-mechanical system,MEMS) 是指内部微结构尺寸在微米甚至纳米量级的微电子机械装置,是一个独立的智能系统. 长宽厚均处于微米量级的微平板为MEMS 中的典型结构,其声学和力学特性直接影响MEMS 的性能. 针对同时受声压激励和气膜力(通过考虑相同尺寸微平板振动引入) 作用的四边简支微平板结构,应用Cosserat 理论和Hamilton 原理,建立了考虑微尺度效应(本征长度和Knudsen 数)影响的声振耦合理论模型,并通过多重Fourier 展开法求解了耦合方程,得到了系统的传声损失结果. 通过频域分析,考虑微平板的不同振动频率、振动幅度和板间距,系统研究了不同尺度效应下微结构中气体薄膜所产生的阻尼力对微平板结构传声特性的影响. 研究发现尺度效应对于微结构的声振特性影响巨大,振动行为对微结构的传声特性也有很大影响,控制并减小微平板的振动幅度以及增大微平板的间距都能够提高微平板的声振性能. 研究结果为MEMS 中微平板的稳定性优化设计提供了理论参考.