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SHEN Hebai ZHOU Wenjun YANG Yongtao & ZHANG FengCollege of Life Environment Science Shanghai Normal University Shanghai China 《中国科学B辑(英文版)》2004,47(1)
The cerium complex bound to the derivative of oligoDNA has been synthesized successfully that can hydrolyze DNA with sequence-specificity. The synthesized derivative of oligoDNA, 5'-EDTA-P-10 mers ODN, was demonstrated by HPLC. The fluorescence spectrum of Tb3+ was detected after its interaction with the hybrid of 10-mers ODN and 26-mers ODN and the results show that the artificial endo-enzyme can recognize and combine firmly with the substrate DNA. The electrophorogram shows that the cerium-oligoDNA hybrid can specifically hydrolyze its substrate DNA and the cleavage site of this hydrolysis reaction is also discussed. This artificial nuclease can be widely used in molecular biology and genetic engineering as one kind of endo-enzyme. 相似文献
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高效液相色谱法检测肉类食品中4种嘌呤碱 总被引:4,自引:1,他引:3
建立了肉类食品中嘌呤含量的多组分高效液相色谱分析方法。采用V(三氟乙酸)∶V(甲酸)∶V(水)=5∶5∶1溶液在90℃下水解样品12min,水解液浓缩后过膜,以0.02mol/LKH2PO4(pH=3.8)为流动相进行色谱分离;流速1.0mL/min;柱温30℃;DAD二极管矩阵检测器检测,检测波长254nm;进样量10μL。在0.4~40mg/L的浓度范围内,各嘌呤的响应峰面积与其相应浓度呈良好相关性,r>0.9999,通过水解条件实验证实,90℃下12min能够使样品中嘌呤完全水解为游离态,且不破坏嘌呤物质,相对标准偏差<16.0%。本方法分离度好,能够快速检测肉类食品中4种主要的嘌呤组分。 相似文献
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水解交联改性的聚丙烯酰胺的吸水保肥能力研究 总被引:1,自引:0,他引:1
研究聚丙烯酰胺(PAM)的水解,并对其结构进行了表征。研究了二醛交联的聚丙烯酰胺及其水解产物对水、肥的吸收及保持能力,结果表明,聚丙烯酰胺水解交联后,部分胺基转化为羧基和羟基。由于羧基、羟基和胺基的协同作用,对水和肥的保持、吸收能力大大增强。水解4h的产物PAM-4中羧基、羟基和胺基的协同作用最好,对水和肥的保持、吸收能力最强。与乙二醛交联的PAM-4相比,戊二醛交联的PAM-4因可以有更大的网络伸展空间,水和肥的保持、吸收能力更强。戊二醛交联的PAM-4对CO(NH2)2有较大的吸收容量。 相似文献
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3-R-Boc-amino-4-(2,4,5-trifluorophenyl)butyric acid (9) was obtained from L-methionine in six steps with a total yield of 32%. The α-amino acid segment of L-methionine was transferred to chiral aziridine by amino protection, reduction, hydroxyl derivation, and cyclization. After ring opening of 2,4,5-trifluoro-phenyl magnesium bromide, the methylthiomethyl group was then hydrolyzed to β-amino alcohol and oxidized to the target β-amino acid. 相似文献
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The cerium complex bound to the derivative of oligoDNA has been synthesized successfully that can hydrolyze DNA with sequence-specificity.
The synthesized derivative of oligoDNA, 5′-EDTA-P-10 mers ODN, was demonstrated by HPLC. The fluorescence spectrum of Tb3+ was detected after its interaction with the hybrid of 10-mers ODN and 26-mers ODN and the results show that the artificial
endo-enzyme can recognize and combine firmly with the substrate DNA. The electrophorogram shows that the cerium-oligoDNA hybrid
can specifically hydrolyze its substrate DNA and the cleavage site of this hydrolysis reaction is also discussed. This artificial
nuclease can be widely used in molecular biology and genetic engineering as one kind of endo-enzyme. 相似文献
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