光纤药物原位溶出度/释放度监测仪实时监测复方氯唑沙宗片体外溶出度

研制了光纤-光学传感-药物溶出监测仪并应用于复方氯唑沙宗片的实时-原位体外溶出度.分支光纤的一端与光源相接,另一端与检测器连接,其公共端部探头浸入于盛于溶出杯中的溶解液中.借联机的计算机进行数据记录及处理,对复方氯唑沙宗片的组分对乙酰氨基酚和氯唑沙宗的回收率在低、中、高三个浓度水平进行了试验,结果依次为98.3%,102.3%和103.1%及108.6%,98.7%和97.7%,其相应的RSD值为1.0%,1.4%和0.4%及1.7%,1.6%及1.3%.应用此监测仪对药片的溶出全过程进行了监控,并显示了药物的溶解曲线,相关的溶出参数可随时提取.试验证明,使用该仪器可获得药物溶出全过程的真实情况的信息.     

Liquid chromatography–tandem MS/MS method for simultaneous quantification of paracetamol,chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic study

A facile, fast and specific method based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) for the simultaneous quantitation of paracetamol, chlorzoxazone and aceclofenac in human plasma was developed and validated. Sample preparation was achieved by liquid–liquid extraction. The analysis was performed on a reversed‐phase C₁₈ HPLC column (5 μm, 4.6 × 50 mm) using acetonitrile–10 mM ammonium formate pH 3.0 (65:35, v/v) as the mobile phase where atrovastatin was used as an internal standard. A very small injection volume (3 μL) was applied and the run time was 2.0 min. The detection was carried out by electrospray positive and negative ionization mass spectrometry in the multiple‐reaction monitoring mode. The developed method was capable of determining the analytes over the concentration ranges of 0.03–30.0, 0.015–15.00 and 0.15–15.00 μg/mL for paracetamol, chlorzoxazone and aceclofenac, respectively. Intraday and interday precisions (as coefficient of variation) were found to be ≤12.3% with an accuracy (as relative error) of ±5.0%. The method was successfully applied to a pharmacokinetic study of the three analytes after being orally administered to six healthy volunteers.     

Simultaneous Determination of Acetaminophen with Orphenadrine Citrate,Ibuprofen or Chlorzoxazone in Combined Dosage Forms by Zero-Crossing Derivative Spectrophotometry

Abstract

A derivative spectrophotmetric procedure for the simultaneous determination of acetaminophen-orphenadrine citrate, acetaminophen-ibuprofen and acetaminophen-chlorzoxazone, binary mixtures is described. The procedure minimises the mutual interference between these drugs in mixtures and allows the determination of these compounds without a previous extraction step. The precision of the method, expressed as the relative standard deviation, is better than 4%. The method has been successfully applied to laboratory mixtures and commercial tablets containing these drugs.     

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Three-Component Tablet Formulation Containing Acetaminophen,Chlorzoxazone, and Aceclofenac

Abstract

The present work describes a simple reversed-phase high-performance liquid chromatographic method that has been developed and validated for simultaneous estimation of acetaminophen, chlorzoxazone, and aceclofenac in tablet dosage form. The estimation was carried out on an Luna C₁₈ (5 µm × 25 cm × 4.6 mm i.d.) column using a mixture of buffer, methanol, and acetonitrile in the ratio 215:130:155 with final pH of 6.5 as a mobile phase, at a flow rate of 1.5 ml/min. Ultraviolet (UV) detection was performed at 275 nm. Total run time was 10 min; these three drugs (acetaminophen, chlorzoxazone, and aceclofenac) were eluted at the retention times of 2.055, 5.096, and 7.605 min respectively. The method was validated for accuracy, precision, linearity, specificity, and sensitivity as per ICH norms.. From the validation study it was found that the method is specific, rapid, accurate, precise, and reproducible. Calibration curves were linear over the concentration ranges of 5–50 µg/ml for acetaminophen and chlorzoxazone, and 5–30 µg/ml for aceclofenac. All the validation study was found statistically significant because all the statistical parameters were within the acceptance range (i.e., COV % < 2.0 and S.D. < 1.0 for both accuracy and precision). The limit of detection (LOD) values were 16.2, 14.6, and 4.8 ng/ml, and LOQ values were 49.0, 46.5, and 14.5 ng/ml for acetaminophen, chlorzoxazone, and aceclofenac respectively. High recovery and low COV % revealed the reliability of the method for quantitative study of three drugs in Micronac-MR tablets. The method is a rapid and cost-effective quality-control tool for routine quantitative analysis of acetaminophen, chlorzoxazone, and aceclofenac in tablet dosage form.     

反相高效液相法同时检测3种探针药物

 用反相高效液相同时测定血清中咖啡因、氨苯砜、氯唑沙宗探针药物的质量浓度,以乙腈 磷酸盐缓冲体系(含0 02mol/L的磷酸二氢钾和0 02mol/L的三乙胺,pH6 5)(体积比为25∶75)为流动相,以安替比林为内标,经C18柱(250mm×4 6mmi d ,5 0μm)分离,紫外检测器检测,使3种探针药物得到较好的分离,并且在有效血药浓度范围内线性良好。该法简便、快速,能够为临床安全有效的用药提供科学的依据。