In vitro anti-denaturation and anti-hyaluronidase activities of extracts and galactolipids from leaves of Impatiens parviflora DC

The in vitro anti-denaturation and anti-hyaluronidase activities of Impatiens parviflora extracts and isolated galactolipids (MGDG-1, DGDG-1) were investigated. This is the first report on these compounds in I. parviflora. All extracts showed anti-hyaluronidase activity, but only methanolic extract from fresh leaves exhibited significant activity against heat-induced denaturation of BSA in a dose-dependent manner. At 500 μg/mL, the extract and the reference drug showed 79.05% and 99.81% inhibition of protein denaturation, respectively. These results indicate that fresh leaves of I. parviflora may be beneficial in inflammatory conditions, especially those associated with protein denaturation, such as rheumatoid arthritis. The study revealed that only MGDG-1 showed weak activity in anti-denaturation assay but both galactolipids were potent inhibitors of hyaluronidase. MGDG-1 completely inhibited the enzyme activity at the concentration of 127.9 μg/mL. These results indicate the potential of galactolipids in the treatment of diseases associated with the loss of hyaluronic acid.     

Variation in phytochemicals and bioactivity of the fruits of Eleutherococcus species cultivated in Poland

Neither the chemical profile and bioactivity of the fruits of Eleutherococcus species cultivated in Poland nor the influence of long-term domestic storage of the fruits are known. No changes in the phenols and flavonoids content in the freshly dried and 1-year storage fruits of Eleutherococcus senticosus and Eleutherococcus henryi were found. The 1-year storage fruits of both species exhibited a very high concentration of Mn, Zn and Cu (mg/kg) compared with the fresh-dried fruits. Spathulenol, β-longipinene and iso-longifolol were predominant essential oil compounds. It was observed that a high capacity of the freshly dried E. senticosus fruits were able to scavenge DPPH^* and inhibit Hyal (EC₅₀ 0.1 mg/mL; IC₅₀ 0.58 mg/mL). Due to their health and pharmacological benefits, a better understanding of the impact of the climate change is important to increase the pharmacological utility of the extracts.