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1.
Antonio R. Montoro Bustos Marta Garcia-Cortes Hector González-Iglesias Jorge Ruiz Encinar José M. Costa-Fernández Miguel Coca-Prados Alfredo Sanz-Medel 《Analytica chimica acta》2015
A generic strategy based on the use of CdSe/ZnS Quantum Dots (QDs) as elemental labels for protein quantification, using immunoassays with elemental mass spectrometry (ICP-MS), detection is presented. In this strategy, streptavidin modified QDs (QDs-SA) are bioconjugated to a biotinylated secondary antibody (b-Ab2). After a multi-technique characterization of the synthesized generic platform (QDs-SA-b-Ab2) it was applied to the sequential quantification of five proteins (transferrin, complement C3, apolipoprotein A1, transthyretin and apolipoprotein A4) at different concentration levels in human serum samples. It is shown how this generic strategy does only require the appropriate unlabeled primary antibody for each protein to be detected. Therefore, it introduces a way out to the need for the cumbersome and specific bioconjugation of the QDs to the corresponding specific recognition antibody for every target analyte (protein). Results obtained were validated with those obtained using UV–vis spectrophotometry and commercial ELISA Kits. 相似文献
2.
Thalia Tsiaka Panagiotis Zoumpoulakis Vassilia J. Sinanoglou Constantinos Makris Georgios A. Heropoulos Antony C. Calokerinos 《Analytica chimica acta》2015
High-energy assisted extraction techniques, like ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE), are widely applied over the last years for the recovery of bioactive compounds such as carotenoids, antioxidants and phenols from foods, animals and herbal natural sources. Especially for the case of xanthophylls, the main carotenoid group of crustaceans, they can be extracted in a rapid and quantitative way with the use of UAE and MAE. 相似文献
3.
Simultaneous quantitative determination of 20 active components in the traditional Chinese medicine formula Zhi‐Zi‐Da‐Huang decoction by liquid chromatography coupled with mass spectrometry: application to study the chemical composition variations in different combinations
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Zheng Tang Ran Yin Kaishun Bi Heyun Zhu Fei Han Kelin Chen Fenrong Wang 《Biomedical chromatography : BMC》2015,29(9):1406-1414
4.
WANG Zhong HONG Haitao XIAO Hai SUN Changku MENG Yan YE Shenghua 《Chinese Journal of Lasers》1997,6(5):435-440
1.IntroductionAbsolutedistancemeasurementisfarfromanewtopic.However,itisstillafieldstimulatinggreatinterestsnowadaysduetoitSimportantroleinmanufacturingandassembly['J.SincethegreatsuccessachievedbyMichelsonandBenoitwhentheyfirstdevelopedaninterferometertodeterminethestandardmeterintermsofthemonochromaticredcadmiumline,theopticinterferometerhasbeenprovedtobeoneofthemostpreciseandefficientwayindisplacementmeasurementbecauseofitshighdiscriminationandsimplestructure.However,thetraditionalinterfe… 相似文献
5.
Harel and Puri (1989, J. Multivariate Anal. 29) studied the asymptotic behavior of the U-statistic and the one-sample rank order statistic for nonstationary absolutely regular processes. In this note, we present some applications of these results for Markov processes as well as ARMA processes. 相似文献
6.
Lihong Wang 《Annals of the Institute of Statistical Mathematics》2004,56(2):251-264
The purpose of this paper is to investigate the asymptotic properties of the least squares estimates (L
2-estimates) and the least absolute deviation estimates (L
1-estimates) of the parameters of a nonlinear regression model subject to a set of equality and inequality restrictions, which
has a long-range dependent stationary process as its stochastic errors. Then we will compare the asymptotic relative efficiencies
of the above estimators. 相似文献
7.
We investigate some topological properties of a normal functorH introduced earlier by Radul which is some functorial compactification of the Hartman-Mycielski construction HM. We prove
that the pair (H X, HMY) is homeomorphic to the pair (Q, σ) for each nondegenerated metrizable compactumX and each denseσ-compact subsetY. 相似文献
8.
对Hajek-Renyi不等式进行了推广,利用推广的不等式给出了随机变量序列绝对平均意义下的强大数定律的一个条件. 相似文献
9.
Dmitry Dolgopyat 《Transactions of the American Mathematical Society》2004,356(4):1637-1689
We consider a large class of partially hyperbolic systems containing, among others, affine maps, frame flows on negatively curved manifolds, and mostly contracting diffeomorphisms. If the rate of mixing is sufficiently high, the system satisfies many classical limit theorems of probability theory.
10.
Stadia are popular models of chaotic billiards introduced by Bunimovich in 1974. They are analogous to dispersing billiards
due to Sinai, but their fundamental technical characteristics are quite different. Recently many new results were obtained
for various chaotic billiards, including sharp bounds on correlations and probabilistic limit theorems, and these results
require new, more powerful technical apparatus. We present that apparatus here, in the context of stadia, and prove “regularity”
properties.
相似文献