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Summary The use of high voltages across a electrophoresis capillary will increase the temperature of the buffer due to Joule heating.
As a result temperature control in CE is rather important since variations in the buffer temperature will result in changes
in the pH of the buffer, peak shape, migration time, reproducibility, efficiency, 3-D structure of macromolecular analytes,
etc. Six different thermostating systems have been evaluated: (i) natural convection, (ii) fan, (iii) home-made and (iv and
v) two commercially available high-speed air and a (vi) liquid thermostated device. In all cases the temperature of the buffer
in the capillary is calculated according to the temperature-conductivity relationship. For this purpose two parameters are
introduced describing temperature control: the temperature onset (δT) and the temperature rise factor (α). From these results, it can be concluded that high speed air thermostating can be as
efficient as liquid thermostating. 相似文献
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