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Amirhossein Nazhand Alessandra Durazzo Massimo Lucarini Maria Alessandra Mobilia Besma Omri 《Natural product research》2020,34(1):110-121
AbstractTaxol is one of the anticancer drugs synthesized naturally in the evergreen Taxus brevifolia forest tree belonging to the yew family (Taxaceae) growing on the Pacific. There are reportedly evidence for treating ovarian, breast and lung cancers through this drug given its unique structural and functional features. Extraction of this drug from yew trees bark is one of the most common ways of producing this drug, but 3000 trees are needed to obtain a kilogram of Taxol. Hence, further attention has recently been attracted to the metabolic engineering strategies, including, engineering cellular metabolism of microorganisms and their optimization. Accordingly, the present paper article was aimed to review recent advances in elevating the production and commercialization of Taxol through metabolic engineering techniques. 相似文献
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Ana Serbanovic Manuel Nunes da Ponte Carlos A.M. Afonso 《Journal of organometallic chemistry》2005,690(15):3600-3608
In this work, osmium-catalyzed asymmetric dihydroxylation (AD) of methyl trans-cinnamate was studied. Osmium and chiral ligand catalysts were immobilized in ionic liquid only, without any other reaction solvents, while the recovery of the product was performed by extraction with supercritical CO2, and compared with results obtained by extractions with organic solvents such as hexane and diethyl ether. In supercritical CO2 extraction experiments, optimal extraction pressure was found and ionic liquid chosen, so that the highest reaction yields coupled with lowest osmium content in the crude product can be achieved. Finally, recycle experiments of the same (ionic liquid + catalytic system) mixture were successfully conducted. Application of ionic liquids and supercritical CO2 in osmium catalyzed AD allows for the isolation of the diol basically without contamination with osmium, in high yield and enantiomeric excess, and it makes possible the efficient reuse of ionic liquid solvent and the catalytic system. 相似文献
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采用mRNA差异显示技术(DDRT—PCR)比较了悬浮培养的中国红豆杉细胞合成紫杉醇前后的基因表达差异,并从紫杉醇合成期细胞中分离出一个特异表达的cDNA克隆TS1.TS1长度为638bp,序列相似性分析结果表明它代表一新基因序列(Genbank登录号:AF426429),将此新基因命名为TS1-FL.开放读框分析结果表明Tsl拥有一个不完整的开放读框,蛋白质同源性检索没有发现与TS1翻译序列有较大同源性的蛋白质.对此基因的进一步研究可揭示它在紫杉醇生物合成中所扮演的角色. 相似文献
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Study on surfactant coating of polymeric nanoparticles for controlled delivery of anticancer drug 总被引:1,自引:0,他引:1
Li?MuEmail author Pei-Hsing?Seow Sheu-Ngo?Ang Si-Shen?Feng 《Colloid and polymer science》2004,283(1):58-65
Biodegradable nanoparticles loaded with anticancer drug paclitaxel and appropriately coated with polyvinyl alcohol (PVA), polyethylene glycol (PEG) as well as d--tocopheryl polyethylene glycol 1000 succinate (TPGS) were produced and characterised by various analysis techniques such as laser light scattering (LLS) for particle size and size distribution, scanning electron microscopy (SEM) and atomic force microscopy (AFM) for particle morphology, X-ray photoelectron spectroscopy (XPS) and Fourier Transform Infrared-Photoacoustic Spectroscopy (FTIR-PAS) for surface chemistry, and high performance liquid chromatography (HPLC) for drug encapsulation efficiency (EE) and in vitro release kinetics. The emphasis was given to the possible effects of surface coating on the physicochemical and pharmaceutical properties of paclitaxel loaded nanoparticles. It was found that the type and amount of the surfactant could significantly affect the drug EE in the nanoparticles, the particles characteristics and their in vitro release behaviour. The surfactants dominated on the nanoparticles surface and the coated nanoparticles displayed in spherical shape with relative smooth surface within the resolution scope of the equipment. The particle size and size distribution showed close relation to the surface coating, which may also be responsible for the drug encapsulation efficiency and the in vitro release kinetics. A favourable formulation of drug loaded nanoparticles of desired properties could be obtained by optimising the fabrication parameters. 相似文献
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During the course of our studies on the taxol chemistry,we have ever tried thereductive cleavage of the acotyl group at 20-C in compound 1a~(1,2) with the hydroxylgroup assistance at 4-C by sodium borohydride. However, the major product 2a~2(27%)still had the acetyl group at 20-C and the signal for the 10-C proton at 6.61 ppm(s)was shifted to 6.31 ppm(d, J=3.79 Hz). A new doublet signal at 3.62 ppm(d, J=3.80 相似文献
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Live morphological analysis of taxol-induced cytoplasmic vacuolization [corrected] in human lung adenocarcinoma cells 总被引:1,自引:0,他引:1
Taxol (paclitaxel), one of the most active cancer chemotherapeutic agents, can cause programmed cell death (PCD) and cytoplasmic vacuolization. The objective of this study was to analyze the morphological characteristics induced by taxol. Human lung adenocarcinoma (ASTC-a-1) cells were exposed to various concentration of taxol. CCK-8 was used to assay the cell viability. Atomic force microscopy (AFM), plasmid transfection and confocal fluorescence microscopy were performed to image the cells morphological change induced by taxol. Fluorescence resonance energy transfer (FRET) was used to monitor the caspase-3 activation in living cells during taxol-induced cell death. Cells treated with taxol exhibited significant swelling and cytoplasmic vacuolization which may be due to endoplasmic reticulum (ER) vacuolization. Caspase-3 was not activated during taxol-induced cytoplasmic vacuolization and cell death. These findings suggest that taxol induces caspase-3-independent cytoplasmic vacuolization, cell swelling and cell death through ER vacuolization. 相似文献