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排序方式: 共有196条查询结果,搜索用时 15 毫秒
1.
This study investigates the effect of surface immobilization on the bactericidal function of a quaternary ammonium compound. Quaternary ammonium silane (QAS) coated planar surfaces did not produce any measurable mortality of Staphylococcus aureus, while 1 µm QAS‐coated microparticles did produce S. aureus mortality. The experiments using QAS‐coated microparticles indicate that the ability of QAS molecules to disrupt the cell wall is not hindered by covalent immobilization of QAS to a surface. These results provide evidence that S. aureus cells on a QAS‐coated planar surface are not exposed to a sufficient number of QAS molecules to produce significant mortality. This result has important implications for the development of self‐decontaminating coatings. Covalent immobilization is used to prevent leaching of the bactericidal compound. However, covalent immobilization may result in a significant tradeoff in bactericidal performance. Published in 2007 by John Wiley & Sons, Ltd. 相似文献
2.
通过不同阳离子和不同阴离子对唾液链球菌嗜热亚种(Streptococcus salivariussub sp.thermophilus)谷氨酸脱羧酶活性的影响以及NaNO3对不同蛋白质浓度的谷氨酸脱羧酶活性的影响进行考察,结果表明:中性盐对谷氨酸脱羧酶活性的影响不是由于增加蛋白质亚基间的疏水作用引起的,而可能是由中性盐对谷氨酸脱羧酶活性的弱抑制作用与中性盐增加了谷氨酸脱羧酶的胶体体系稳定性共同作用所致,同时还可能与中性盐降低了反应体系CO2的溶解度有关. 相似文献
3.
M. van Raamsdonk H.C. van der Mei G.I. Geertsema-Doornbusch J.J. de Soet H.J. Busscher J. de Graaff 《Colloids and surfaces. B, Biointerfaces》1995,4(6):401-410
Deposition of the oral bacteriumStreptococcus sobrinus HG977 onto glass (water contact angle 0°) and onto FEP-Teflon (fluoroethylenepropylene; water contact angle 110°) was studied in a parallel-plate flow chamber in the presence and absence of polyclonal antibodies (pAb) and monoclonal antibodies (mAbs) adsorbed onto the cells. The zeta potentials of the bacteria ranged from −7.1 to −8.5 mV at pH 6.8 and were not affected by the presence of pAb or mAbs. Hydrophobicity (by water contact angles) increased from 30° (no antibodies) to 88° in the presence of pAb adsorbed onto the bacterial cell surface. The untreatedS. sobrinus had a greater tendency to adhere to glass (44.5 × 106 cm−2) than to FEP-Teflon (18.3 × 106 cm−2), in accordance with thermodynamic modelling. After preincubation ofS. sobrinus with pAb, its clear preference for adhesion to glass disappeared as expected from its increased hydrophobicity. Although forS. sobrinus preincubated with OMVU10 no difference was found in hydrophobicity in comparison to the untreated bacteria, the number of bacteria adhering to glass decreased to 10.2 ¢ 106 cm−2. Formation of bacterial aggregates was found whenS. sobrinus, preincubated with pAb or OMVU10, adhered to glass and FEP. This was also observed when untreated bacteria adhered to glass coated with OMVU10, or to FEP coaled with OMVU10 or pAb. Adhesion in these experiments is therefore thought to occur via near-neighbour collection induced by the presence of pAb or mAbs. Low numbers of bacteria were removed from glass after draining the flow cell, whereas high numbers of untreated bacteria and bacteria preincubated with OMVU10 were removed from FEP.S. sobrinus cells preincubated with pAb were not removed but piled up. It was concluded that the adhesion of untreatedS. sobrinus andS. sobrinus preincubated with pAb is in accordance with thermodynamic modelling, based on the overall wettability of the cell surfaces, whereas the adhesion ofS. sobrinus preincubated with OMVU10 may be through localized interactions, not expressed in overall surface properties. 相似文献
4.
钇离子及其阳离子卟啉配合物与金黄色葡萄球菌的相互作用 总被引:14,自引:0,他引:14
用LKB-2277生物活性检测系统考察了Y~(3+),TMP及其阳离子型钇卟啉配合物 {[Y(TMP)(H_2O)_3]Cl, TMP = 5, 10, 15, 20-四(4-甲氧基苯基)卟啉}对金黄色 葡萄球菌全程代谢作用的影响,测定了Y~(3+),TMP和[Y(TMP)(H_2O)_3]Cl对金黄 色葡萄球菌作用的产热曲线,根据产热曲线求算了金黄色葡萄球菌在Y~(3+),TMP ,[Y(TMP)(H_2O)_3]Cl作用下生长代谢的速率常数k_1,k_2,抑制率I_1,I_2,和 半抑制浓度IC_(50)~1,IC_(50)~2等热动力学参数。实验结果表明:Y~(3+),[Y (TMP)(H_2O)_3]Cl对金黄色葡萄球菌的生长代谢有双向调节作用,在低浓度下表现 为刺激作用,高浓度为抑制作用,而TMP对金黄色葡萄球菌的生长代谢主要为抑制 作用,在低浓度下表现为刺激作用,高浓度为抑制作用,而TMP对金黄色葡萄球菌 的生长代谢主要为抑制作用,对金黄色葡萄球菌的抑制作用[Y(TMP)(H_2O)_3]Cl > TMP > Y~(3+)。 相似文献
5.
Ali Reza Kazemizadeh Nahid Shajari Reza Shapouri Neda Adibpour Reza Teimuri‐Mofrad Parisa Dinmohammadi 《应用有机金属化学》2016,30(3):148-153
A four‐component reaction between aromatic carboxylic acids, (N‐isocyanimino)triphenylphosphorane, ferrocenecarbaldehyde and dibenzylamine is reported. This approach is an efficient, simple and high‐yield procedure for the synthesis of 1,3,4‐oxadiazole derivatives containing a ferrocene unit. The antimicrobial activities of the products were investigated against Staphylococcus aureus and Pseudomonas aeruginosa in in vitro and in vivo assays. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
6.
T. V. Subramanya Prasad C. R. Shahini Shivaputra A. Patil Xiaojun Huang 《Journal of Coordination Chemistry》2017,70(4):600-614
Various nitro/nitrile-functionalized benzimidazol-2-ylidene carbene complexes of silver(I) (7a–d and 11a–d) were synthesized by combination of 1-allyl/1-isopropyl/1-sec-butyl/1-isopentyl-3-(nitro/cyano-benzyl)-3H-benzimidazol-1-ium hexafluorophosphate (6a–d and 10a–d) with silver(I) oxide in acetonitrile. The compounds were characterized by 1H, 13C NMR, FT-IR, mass spectrometry, and elemental analysis. Additionally, the in vitro antibacterial activity of the N-heterocyclic carbene (NHC) precursors (6a–d and 10a–d) and their corresponding NHC-silver(I) complexes (7a–d and 11a–d) were investigated against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli using the qualitative Kirby-Bauer disk diffusion method. All the NHC-silver(I) complexes exhibited medium-to-high antibacterial activity with areas of clearance ranging from 12 mm to 21 mm, while the NHC precursors were inactive against both strains of bacteria. 相似文献
7.
Basma Najar Giulia Mecacci Valeria Nardi Claudio Cervelli Simona Nardoni Francesca Mancianti Valentina Virginia Ebani Simone Giannecchini Luisa Pistelli 《Molecules (Basel, Switzerland)》2021,26(9)
Spontaneous emissions of S. dentata Aiton and S. scabra Thunb., as well as the essential oil (EO) composition of the cited species, together with S. aurea L., were investigated. The chemical profile of the first two species is reported here for the first time. Moreover, in vitro tests were performed to evaluate the antifungal activity of these EOs on Trichophyton mentagrophytes, Microsporum canis, Aspergillus flavus, Aspergillus niger, and Fusarium solani. Secondly, the EO antibacterial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus pseudointermedius was examined, and their antiviral efficacy against the H1N1 influenza virus was assessed. Leaf volatile organic compounds (VOCs), as well as the EOs obtained from the arial part of Salvia scabra, were characterized by a high percentage of sesquiterpene hydrocarbons (97.8% and 76.6%, respectively), mostly represented by an equal amount of germacrene D (32.8% and 32.7%, respectively). Both leaf and flower spontaneous emissions of S. dentata, as well as the EO composition, showed a prevalence of monoterpenes divided into a more or less equal amount of hydrocarbon and oxygenated compounds. Interestingly, its EO had a non-negligible percentage of oxygenated sesquiterpenes (29.5%). S. aurea EO, on the contrary, was rich in sesquiterpenes, both hydrocarbons and oxygenated compounds (41.5% and 33.5%, respectively). S. dentata EO showed good efficacy (Minimal Inhibitory Concentration (MIC): 0.5%) against M. canis. The tested EOs were not active against E. coli and S. aureus, whereas a low inhibition of S. dentata EO was observed on S. pseudointermedius (MIC = 10%). Once again, S. dentata EO showed a very good H1N1 inhibition; contrariwise, S. aurea EO was completely inactive against this virus. The low quantity of S. scabra EO made it impossible to test its biological activity. S. dentata EO exhibited interesting new perspectives for medicinal and industrial uses. 相似文献
8.
《Arabian Journal of Chemistry》2020,13(2):3988-3996
In the current study, gold nanoparticles (AuC6NPs and AuC8NPs) were prepared through sodium borohydride reduction method by using Calix[6]rene and Calix[8]rene as a stabilizing agents. The synthesized AuNPs were screened for cytotoxic, phytotoxic, antifungal and antibacterial activities. The fabricated AuNPs were characterized by UV–visible spectroscopy, atomic force microscopy (AFM) and FTIR spectroscopy. Antibacterial activities of the AuNPs were tested against E. coli and S. aureus. The AuC6NPs were found to be effective against the growth of gram positive bacteria and inhibited the growth of S. aureus. AuC6NPs interact with bacterial cell and damaged cell membrane. Roughness of the bacterial surface and membrane rupture can be clearly observed by AFM images. The AuNPs possess insignificant antifungal activity against Aspergillus niger and Candida albicans. Moreover, AuC8NPs have significant phytotoxicity and moderate cytotoxicity. 相似文献
9.
Michio Kurosu Katsuhiko Mitachi Junshu Yang Edward V. Pershing Bruce D. Horowitz Eric A. Wachter John W. Lacey III Yinduo Ji Dominic J. Rodrigues 《Molecules (Basel, Switzerland)》2022,27(1)
Rose bengal has been used in the diagnosis of ophthalmic disorders and liver function, and has been studied for the treatment of solid tumor cancers. To date, the antibacterial activity of rose bengal has been sporadically reported; however, these data have been generated with a commercial grade of rose bengal, which contains major uncontrolled impurities generated by the manufacturing process (80–95% dye content). A high-purity form of rose bengal formulation (HP-RBf, >99.5% dye content) kills a battery of Gram-positive bacteria, including drug-resistant strains at low concentrations (0.01–3.13 μg/mL) under fluorescent, LED, and natural light in a few minutes. Significantly, HP-RBf effectively eradicates Gram-positive bacterial biofilms. The frequency that Gram-positive bacteria spontaneously developed resistance to HP-RB is extremely low (less than 1 × 10−13). Toxicity data obtained through our research programs indicate that HP-RB is feasible as an anti-infective drug for the treatment of skin and soft tissue infections (SSTIs) involving multidrug-resistant (MDR) microbial invasion of the skin, and for eradicating biofilms. This article summarizes the antibacterial activity of pharmaceutical-grade rose bengal, HP-RB, against Gram-positive bacteria, its cytotoxicity against skin cells under illumination conditions, and mechanistic insights into rose bengal’s bactericidal activity under dark conditions. 相似文献
10.
Koutaro Shiraishi Hironobu Koseki Toshiyuki Tsurumoto Koumei Baba Mariko Naito Koji Nakayama Hiroyuki Shindo 《Surface and interface analysis : SIA》2009,41(1):17-22
Photocatalysis with anatase Titanium dioxide (TiO2) under ultraviolet A (UVA) has a well recognized bactericidal effect. There have been a few reports, however, on the effects of photocatalysis on bio‐implant‐related infections. The purpose of present study was to evaluate the photocatalytic bactericidal effects of anatase TiO2 on Staphylococcus aureus (S. aureus) associated with surgical site infections. TiO2 films were synthesized on commercially pure titanium substrates and SUS316 stainless steel using a plasma source ion implantation method followed by annealing. The chemical composition of the surface layers was determined using GXRD and XPS. The disks were seeded with cultured S. aureus and exposed to UVA illumination from black light. The bactericidal effect of the TiO2 films was evaluated by counting the survived colonies statistically. A structural gradient anatase type TiO2 layer formed on all substrates. The viability of the bacteria on the photocatalytic TiO2 film coated on titanium was suppressed to 7.0% at 30 minutes and 5.5% at 45 minutes, whereas that on a similarly coated stainless steel was suppressed to 45.8% at 30 minute and 28.6% at 45 minutes (ANOVA: p < 0.05). Complete bacterial inactivation was achieved after 90 minutes on titanium and after 60 minutes on stainless steel. The photocatalytic bactericidal effect of TiO2 is useful for sterilizing the contaminated surfaces of bioimplants. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献