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1.
A novel triterpenoid 3α,16β,23,24-tetrahydroxy-28-nor-ursane-12,17,19,21-tetraen (1) was isolated from the fruits of Gardenia jasminoides var. radicans Makino. The structure of the new compounds was elucidated on the basis of spectroscopic analysis including MS and NMR data. Compound 1 was in vitro tested for cytostatic activity on human throat cancer (Hep-2) cell line by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide method and showed mild anticancer activity with the IC50 of 31.2 μM. 相似文献
2.
Wei-Wei Jiang Jia Su Xing-De Wu Juan He Li-Yan Peng Xiao Cheng 《Natural product research》2015,29(9):842-847
Geissoschizine methyl ether N-oxide, a new oxindole alkaloid, along with 14 known alkaloids, was isolated from the aerial part of Uncaria rhynchophylla. Their structures were identified by comprehensive spectral methods, including 2D NMR experiments, and confirmed by comparing with the literature data. In vitro acetylcholinesterase (AChE) inhibitory activity assay showed that the new compound exhibited anti-AChE activity with IC50 value of 23.4 μM. 相似文献
3.
Two new guaiane-type sesquiterpenoid glucosides (1 and 2) were isolated from the fruit of Gardenia jasminoides Ellis. Their structures were elucidated to be (1R,7R,10S)-11-O-β-D-glucopyranosyl-4-guaien-3-one (1) and (1R,7R,10S)-7-hydroxy-11-O-β-D-glucopyranosyl-4-guaien-3-one (2) by one- and two-dimensional NMR techniques ((1)H NMR, (13)C NMR, HSQC, HMBC and NOESY), MS, CD spectrometry and chemical methods. 相似文献
4.
HPTLC silica gel plates without and with fluorescence indicator F254 in combination with n-hexane–ethyl acetate–formic acid (20:19:1, v/v/v) as a developing solvent were explored for the HPTLC–densitometric and HPTLC–MS/(MSn) analyses of flavonoids. Pre-development of the plates with chloroform–methanol (1:1, v/v) was needed for reliable HPTLC–densitometric analyses of flavonoid aglycones in the whole RF range, while 2-step pre-development (1st methanol–formic acid (10:1, v/v), 2nd methanol), that decreased background signals of formic acid adducts, was required for HPTLC–MS analyses. Optimization with conditioning of the adsorbent layer with water before development and saturation of the twin trough chamber resulted in required decrease of the RF values of studied flavonoids (flavone, apigenin, luteolin, chrysin, quercetin dihydrate, myricetin, kaempferide, kaempferol, naringenin, pinocembrin).Detection was performed based on fluorescence quenching (on the plates with F254), natural fluorescence and after post-chromatographic derivatization with natural product reagent without or with further enhancement and stabilization of fluorescent zones with polyethylene glycol (PEG 400 or PEG 4000) or paraffin–n-hexane reagents. For all three reagents, drying temperature and time passed after drying influenced the intensity, which was increasing the first 20?min, and the stability (less than 2?h for PEGs and at least 24?h for paraffin–n-hexane) of the standards’ zones.Optimal wavelengths for densitometric evaluation were selected based on in-situ absorption spectra scanned before and after derivatization and after stabilization. The developed method was tested via analyses of propolis, roasted coffee, rose hip, hibiscus, rosemary and sage crude extracts. To further increase the reliability of the obtained densitometric results HPTLC–MS/(MSn) analyses of all crude extracts were performed. Several phenolic and non-phenolic compounds were tentatively identified.Some possible interferences with phenolic acids (chlorogenic acid, rosmarinic acid, protocatechuic acid, gallic acid, syringic acid, ellagic acid, trans-cinnamic acid, o-coumaric acid, m-coumaric acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid) that are often present in the extracts together with flavonoids were also examined. 相似文献
5.
Wei-Wu Song Xue-Qin Wang Hua-Li Jia Zheng-Kun Zhang Yong-Jie Ding Ya-Hong Chen 《Natural product research》2018,32(19):2338-2342
The plants in the genus Gardenia (Rubiaceae) have long been used as traditional medicines in China. In this study, two new 3,4-seco-cycloartane triterpenes, sootepin J (1) and sootepin K (2), and a novel nor-3,4-seco-cycloartane triterpenes, sootepin L (3), together with two known compounds (4–5), were isolated from the methanolic extract of the leaves and twigs of Gardenia sootepensis. The structures of the new compounds were elucidated by combinations of 1D, 2D NMR experiments and HR-MS data, while the known compounds were identified by comparison of the NMR data with previously published data. 相似文献
6.
Nguyen Phuong Hanh Nguyen Huu Toan Phan Nguyen Thi Dieu Thuan Tran Thi Hong Hanh Le Thi Vien Nguyen Phuong Thao 《Natural product research》2016,30(18):2071-2076
Six iridoid derivatives (1–6), including two new compounds myrmecodoides A and B (1 and 2), were isolated from the ant-plant Myrmecodia tuberosa. Their structures were determined on the basis of spectroscopic data (1H and 13C NMR, HSQC, HMBC, 1H-1H COSY, NOESY and HR-ESI-MS) and by comparison with the literature values. Among isolates, 3 and 4 exhibit weak antibacterial effect against Staphylococcus aureus subsp. aureus with MIC value of 100.0 μg/mL. 相似文献
7.
Veronique Sinou Julien Fiot Nicolas Taudon Joël Mosnier Maryse Martelloni Sok S. Bun Daniel Parzy Evelyne Ollivier 《Journal of separation science》2010,33(12):1863-1869
In Africa, Mitragyna inermis (Willd.) O. Kuntze (Rubiaceae) is commonly used in traditional medicine to treat malaria. Antimalarial activity is mostly due to the hydromethanolic extract of M. inermis leaves and especially to the main alkaloids, uncarine D and isorhynchophilline. In the present study, we describe for the first time an HPLC method for the simultaneous quantification of uncarine D and isorhynchophylline in biological matrices. SPE was used to extract the components and the internal standard naphthalene from human and pig plasma samples. Chromatographic separation was performed on a C‐18 reversed column at a flow rate of 1 mL/min, using methanol–phosphate buffer (10:90, pH 7), as a mobile phase. Good linearity was observed over the concentration ranges of 0.0662–3.31 μg/mL for uncarine D and 0.0476–2.38 μg/mL for isorynchophylline. The precision was less than 12% and the accuracy was from 86 to 107% without any discrepancy between the two species. Uncarine D and isorhynchophylline recoveries were over 80%. These results allowed the quantification of both uncarine D and isorhynchophylline in pig plasma after intravenous administration of M. inermis extract. 相似文献
8.
Nine compounds, ursolic acid (1), β-sitosteryl glucoside (2), umbelliferone (3), uncarine-A (4), chlorogenic acid (5), rutin (6), neohesperidin (7), quercitrin (8)and afzelin (9) were isolated and characterized from fresh leaves of Uncaria hirsuta. Their structures were elucidated by spectral methods. Among them, uroslic acid (I) exhibited a cytotoxic principle of this plant. 相似文献
9.
From the root bark ofGardenia imperialis two triterpenoids were isolated and identified as pulcherrol (= 3-epi--amyrin) and -amyrin acetate.
Triterpenoide vonGardenia imperialis (Kurze Mitteilung)
Zusammenfassung Aus der Rinde der Wurzeln vonGardenia imperialis wurden zwei Triterpene isoliert, die als Pulcherrol (= 3-epi--Amyrin) und -Amyrinacetat identifiziert wurden.相似文献
10.
A naphthohydroquinone and eight anthraquinones were isolated from the roots of Rubia lanceolata Hayata. Their structures were assigned as mollugin ( 1 ), l-hydroxy-2-methyl-9,10-anthraquinone ( 2 ), 2-methylquinizarin ( 3 ), 3-carbomethoxy-l-hydroxy-9,10-anthraquinone ( 4 ), lucidin ethyl ether ( 5 ), rubiadin ( 6 ), alizarin ( 7 ), ω-hydroxypachybasin ( 8 ) and digiferruginol ( 9 ) on the basis of spectral evidence. Among them, compounds 8 and 9 were isolated for the first time from Rubia species. 相似文献