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Zhi Zhu Zhichao Guan Dan Liu Shasha Jia Jiuxing Li Zhichao Lei Shuichao Lin Tianhai Ji Zhongqun Tian Chaoyong James Yang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2015,127(36):10594-10599
Herein, we demonstrate that a very familiar, yet underutilized, physical parameter—gas pressure—can serve as signal readout for highly sensitive bioanalysis. Integration of a catalyzed gas‐generation reaction with a molecular recognition component leads to significant pressure changes, which can be measured with high sensitivity using a low‐cost and portable pressure meter. This new signaling strategy opens up a new way for simple, portable, yet highly sensitive biomedical analysis in a variety of settings. 相似文献
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《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2018,130(31):9850-9854
It is recognized that biocomputing can provide intelligent solutions to complex biosensing projects. However, it remains challenging to transform biomolecular logic gates into convenient, portable, resettable and quantitative sensing systems for point‐of‐care (POC) diagnostics in a low‐resource setting. To overcome these limitations, the first design of biocomputing on personal glucose meters (PGMs) is reported, which utilizes glucose and the reduced form of nicotinamide adenine dinucleotide as signal outputs, DNAzymes and protein enzymes as building blocks, and demonstrates a general platform for installing logic‐gate responses (YES, NOT, INHIBIT, NOR, NAND, and OR) to a variety of biological species, such as cations (Na+), anions (citrate), organic metabolites (adenosine diphosphate and adenosine triphosphate) and enzymes (pyruvate kinase, alkaline phosphatase, and alcohol dehydrogenases). A concatenated logical gate platform that is resettable is also demonstrated. The system is highly modular and can be generally applied to POC diagnostics of many diseases, such as hyponatremia, hypernatremia, and hemolytic anemia. In addition to broadening the clinical applications of the PGM, the method reported opens a new avenue in biomolecular logic gates for the development of intelligent POC devices for on‐site applications. 相似文献
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Andreas Humeny 《Chemie in Unserer Zeit》2003,37(6):380-387
The high accuracy, molecular resolution and sensitivity of matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) make it an efficient method for analysing all kinds of biomolecules including nucleic acids, proteins/peptides, carbohydrates and lipids. MALDI‐TOF‐MS based high‐throughput genotyping of genetic heterogeneities possesses the potential of becoming a routine method. MAL‐DI‐TOF‐MS can be used for the identification of proteins and posttranslational modifications. Taken together, MALDI‐TOF‐MS represents a integrated platform technology in bioanalytics and molecular medicine. 相似文献
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