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SUI Yu-jie ZHANG Gui-zhen WANG Qian WANG Ya-li WU Mei DU Zhen-wu ZHANG Jie JIANG Ri-hua . Central Laboratory China-Japan Union Hospital of Jilin University Changchun P. R. China . Central Laboratory the Second Hospital of Jilin University Changchun 《高等学校化学研究》2011,(2):277-281
A relatively sensitive, specific, and photostable method for the detection of cytokeratin of cancer cells via conjugation with cadmium telluride quantum dots(CdTe QDs) was described. Water soluble CdTe QDs were conjugated to anti-pan-cytokeratin(CK) monoclonal antibody(MAb) through coupling reagent [1-ethyl-3-(3-dimethyla- mino propyl)carbodiimide, EDC] and the conjugates were purified by dialysis. The expression of pan CK protein in HepG2 cells was observed by immunocytochemistry and direct immunofluoresce... 相似文献
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