Rapid Purification and Identification of Osteopontin from Human Milk by HPLC Coupled to FT-ICR-MS

Milk as a key element for infant nutrition represents the only source of feeding for newborns and infants, breast-feeding milk normally contains several bioactive proteins or peptides useful for the development of the immune system that protects infants from diseases. Osteopontin(OPN) plays a distinct role during the processes of lactation. Some studies on OPN isolated and purified from the human milk via HPLC on SCX and C4 columns adopted biological mass spectrometry. After digesting the purified OPN sampl...     

超声参数联合血清OPN、IL-1β对乳腺癌的临床诊断价值研究

选取我院收治的乳腺癌(BC)患者82例设为研究组,同期乳腺良性肿瘤患者82例设为对照组,探讨了超声参数联合血清骨桥蛋白(OPN)、白细胞介素-1β(IL-1β)对BC的诊断价值.结果显示,研究组患者病灶处的RI值、PI值和血清OPN、IL-1β水平均高于对照组(P＜0.05);PI值、RI值、血清OPN、IL-1β水平...     

Identification of Key Candidate Genes Involved in the Progression of Idiopathic Pulmonary Fibrosis

Idiopathic pulmonary fibrosis (IPF) is a lethal, agnogenic interstitial lung disease with limited therapeutic options. To investigate vital genes involved in the development of IPF, we integrated and compared four expression profiles ({"type":"entrez-geo","attrs":{"text":"GSE110147","term_id":"110147"}}GSE110147, {"type":"entrez-geo","attrs":{"text":"GSE53845","term_id":"53845"}}GSE53845, {"type":"entrez-geo","attrs":{"text":"GSE24206","term_id":"24206"}}GSE24206, and {"type":"entrez-geo","attrs":{"text":"GSE10667","term_id":"10667"}}GSE10667), including 87 IPF samples and 40 normal samples. By reanalyzing these datasets, we managed to identify 62 upregulated genes and 20 downregulated genes in IPF samples compared with normal samples. Differentially expressed genes (DEGs) were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis to illustrate relevant pathways of IPF, biological processes, molecular function, and cell components. The DEGs were then subjected to protein–protein interaction (PPI) for network analysis, serving to find 11 key candidate genes (ANXA3, STX11, THBS2, MMP1, MMP9, MMP7, MMP10, SPP1, COL1A1, ITGB8, IGF1). The result of RT-qPCR and immunohistochemical staining verified our finding as well. In summary, we identified 11 key candidate genes related to the process of IPF, which may contribute to novel treatments of IPF.