ICP-AES测定野生羌活药材中K、Na和Al

利用电感耦合等离子体-原子发射光谱法（ICP-AES）测定不同采收时间（5、6、7、8月和9月）野生羌活药材中K、Na、Al 3种金属元素的含量,应用SPSS16.0分析软件对结果进行统计分析。研究结果表明,5—9月野生羌活药材中均以K元素含量最高;K和Na、Na和Al的含量呈负相关,K和Al的含量呈正相关;K元素的含量在5月到6月增加,6月到8月呈下降趋势,8月到9月急剧升高;Na元素的含量呈先上升（5—6月）后下降（6—9月）的趋势;Al元素的含量呈先降低（5—6月）再升高（6—9月）的趋势。     

应用2D NMR技术研究羌活苷的结构

从传统中药羌活中分离得到1个香豆素类化合物,应用1D和脉冲梯度场反相2D NMR检测技术 (gCOSY, gNOESY, gHMQC, gHMBC) 研究了其结构,鉴定为6′-O-(反式阿魏酰基)-紫花前胡苷,命名为羌活苷（Forbesoside）. 本文对其碳氢NMR信号进行了全归属. 对从伞形花内酯衍生化为7-去甲基软木花椒素、紫花前胡苷元和6′-O-(反式阿魏酰基)-紫花前胡苷的NMR信号变化规律以及糖残基NMR信号归属方法进行了讨论和总结.     

青海高原道地药材羌活和铁棒锤微量元素含量比较研究

为研究青海高原道地药材羌活和铁棒锤的特性,采用电热板消解法处理样品,原子吸收分光光度法测定了两种药材中Ca、Mg、Cu、Zn、Fe、Mn 6种金属元素的含量。结果表明,Ca、Mg、Cu、Zn、Fe、Mn 6种微量元素含量在两种道地药材中,含量最高的是Ca,平均值分别为(55.78±12.10)mg/g和(26.48±5.79)mg/g;含量最低的元素为Cu,其值分别为(0.28±0.28)mg/g和(0.09±0.06)mg/g;其它元素含量从高到低依次为Mg,Fe,Zn,Mn。可见不同地区的羌活其微量元素的含量有明显的差异,且羌活微量元素含量也存在种间差异,细叶羌活的Ca、Mg元素含量比宽叶羌活的要低,相反,Fe,Cu,Zn 3种元素含量细叶羌活的比宽叶的要高。     

超临界二氧化碳萃取羌活研究初探

研究了用超临界二氧化碳法萃取羌活的工艺条件,探讨了萃取压力、温度、时间对萃取效果的影响。萃取物最高收率为８．７５％,比水蒸汽蒸馏法收率高六倍以上。     

A new dimeric furanocoumarin from Notopterygium incisum

A new dimeric psoralen-type furanocoumarin, notopterol-（18-O-20＇）-notopol （1）, was isolated from Notopterygium incisum for the first time and its structure was elucidated by spectroscopic methods.     

Quantitative Determination of the Chemical Profile of the Plant Material “Qiang-huo” by LC-ESI-MS-MS

The rhizomes of Notopterygium incisum Ting. ex H. T. Chang and N. forbesii Boiss. are recorded in the pharmacopoeia of the People’s Republic of China under the same name “Qiang-huo”. Valid quality control of Qiang-huo is desirable due to the fact that the wild natural sources of Qiang-huo have almost been exhausted and large regulated cultivation is developing. In the present paper, HPLC fingerprinting was developed to identify and distinguish both species in detail. The unique properties of the HPLC fingerprint were validated by analyzing 15 batches of N. incisum and 11 batches of N. forbesii. A standardized procedure involving liquid chromatography–electrospray ionization–multiple stage mass spectrometry (HPLC-ESI-MSⁿ) was developed to identify the fingerprint components, and a total of eight characteristic peaks were unequivocally identified. An average chromatogram from 15 batches of N. incisum from different geographic sources, considered to be the original and genuine herbal medicine, was first established as the standard fingerprint. The chemical profiles of 11 batches of N. forbesii samples were found to be variable. The HPLC method can differentiate N. incisum from N. forbesii by either the marker compounds notoptol (4) and p-hydroxypenethylanisate (8) or the amounts of nodakenin (1), notopterol (5), and 6′-O-trans-feruloylnodakenin (7). The HPLC fingerprint analysis is specific and may serve for quality identification and comprehensive evaluation of Qiang-huo.