THE SEPARATION,PURIFICATION AND DECOLORIZATION OF NEW ANTIBIOTICS-MILUPEINAN USING ADSORPTION RESIN

1. INTRODUCTION Fig.1 The Chemical Structure of Milupeinan As a new type of antibiotics Milupeinan, not only possesses antibacterial activities against a wide range of Gram-positive and Gram-negative bacteria, but also higgh stability under th…     

THE SEPARATION，PURIFICATION AND DECOLORIZATION OF NEW ANTIBIOTICS—MILUPEINAN USING ADSORPTION RESIN

Adsorption resin AAS was used for the separation and purification of milupeinan (M-13)from its mother liquor. The good adsorption property of AAS adsorption resin was found formilupeinan (M-13) compared with the Diaion CHP 20p. When acetone and water (2:1 in volume)were used as desorption reagents, it was found that 3 times of bed volume of the desorption reagentswould make the AAS resin which was saturately adsorpted M-13 desorption completely.Also a macroporous anion exchange resin (Poly-4-vinylpyridine type) D-280 was used for thedecolourization from M-13 solution. Good results was given when the sr=1～2.     

树脂法分离纯化米洛培南

采用国产AAS大孔吸附树脂及D－280大孔脱色树脂对抗生素米洛培南制备母液进行分离纯化。实验表明，与日本Diaion公司生产的Diaion CHP20相比，AAS大孔吸附树脂对米洛培南具有更优良的吸附性能，使用D－280大孔脱色树脂对抗生素米洛培南制备母液进行脱色，未发现类似报道，分析结果证实，以上述方法制备的抗生素米洛培南收率高，纯度高，各项分析数据与文献报道相符。