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Mayya Sundukova Efthymia Prifti Annalisa Bucci Kseniia Kirillova Joana Serrao Luc Reymond Miwa Umebayashi Ruud Hovius Howard Riezman Kai Johnsson Paul A. Heppenstall 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(8):2363-2366
Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash‐free imaging and faithfully detects supra‐ and sub‐threshold activity in neurons. 相似文献
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