Simultaneous determination of lappaconitine hydrobromide and isopropiram fumarate in rabbit plasma by capillary electrophoresis with electrochemiluminescence detection

A CE electrochemiluminescence (CE-ECL) method for simultaneous determination of lappaconitine hydrobromide (LH) and isopropiram fumarate (IF) has been first established, with a chemically modified platinum electrode by europium (III)-doped Prussian blue analogue film as a working electrode. The conditions for CE separation and ECL detection are discussed and optimized in detail. It has been proved that 20 mmol/L phosphate buffer (pH 8.5) containing 5% (v/v) ACN and 0.17 mol/L SDS could achieve the most favorable resolution, and the high sensitivity of detection was obtained by maintaining the detection potential at 1.23 V. Under optimized conditions, a baseline separation for the two analytes was achieved within 6 min, and the standard curves were linear in the range of 1.0×10(-7) ～ 5.0 × 10(-5) g/mL for LH and 4.0 × 10(-8) ～ 1.0 × 10(-5) g/mL for IF with the detection limits (3σ) of 6.6 × 10(-8) g/mL for LH and 3.7 × 10(-8) g/mL for IF, respectively. The precisions of intra- and interday measurements for LH and IF were less than 4.21 and 2.61%, respectively. The applicability of the proposed method was illustrated in the determination of LH and IF in rabbit plasma with recoveries between 95.6 and 103.0%.     

新疆丰产伊犁翠雀化学成分研究

为了开发新疆的伊犁翠雀(Del phinium Iliense Huth),我们对其化学成分进行了研究,分离得到8种晶体,均为在该植物中首次发现,其中刺乌碱含量较高,并可作为非成瘾镇痛药(多用于晚期癌痛)用于临床,该发现为开发利用这一有毒野生植物,提供了科学依据。 1 仪器 显微熔点仪;NICOLET-5DX FTIR光谱仪,KBr压片;日本岛滓UV-     

Phototransformation products of the alkaloid lappaconitine: Multinuclear NMR study

The NMR technique has been applied to characterize the phototransformation products of the natural alkaloid lappaconitine. It was demonstrated that the photolysis of lappaconitine 1 results in cleavage of the ester bond with elimination of N-acetylanthranilic acid. The final reaction product was found to be an immonium salt 4 of N-acetylanthranilic acid and enamine 3. An equilibrium between the imine cation and the enamine 3 was detected.