海蜇中微量汞的微波消解-原子荧光光谱法测定

以冷冻干燥和微波消解相结合的方式处理样品 ,采用冷原子荧光光谱法测定了新鲜海蜇和腌制海蜇不同部位的汞含量。通过试验 ,确定了最佳的样品处理条件和仪器的工作条件。方法简便、快速、准确。检出限为0.015ng·mL -1 ,RSD(n=5)为2.3%～4.2 %,回收率为95%～104%。     

微波消解-氢化物发生-原子荧光法测定海蜇中的痕量砷和硒

建立了氢化物发生-原子荧光光谱法(HG-AFS)测定新鲜和腌制海蜇中痕量砷、硒的分析方法。采用冷冻干燥和微波消解相结合的方法处理样品,设定了最佳的样品处理条件和仪器测定条件,解决了消解液中过剩酸对测定的干扰问题,并对共存元素干扰情况进行了研究。利用加标回收试验,对分析方法进行了验证。实验结果表明,该方法具有简便、快速、灵敏、准确等特点,适合于含水量较高的生物样品的测定。     

海蜇不同部位脂肪酸的组成研究

报道了黄海海域成熟海蜇不同部位脂肪酸的研究结果。利用GC-MS技术测定海蜇皮、海蜇头、海蜇生殖腺的脂肪酸组成。结果表明：海蜇含有30多种脂肪酸,3个部位的脂肪酸组成差别不大;海蜇中含有丰富的多不饱和脂肪酸,含量在36．2％～38．7％之间,其中二十二碳六烯酸(DHA)、二十碳四烯酸(AA)和二十碳五烯酸(EPA)含量较高。     

绿色荧光蛋白质(GFP)的发现、表达和发展

本文简要地介绍了2008年Nobel化学奖——绿色荧光蛋白质的主要学术内容,包括绿色荧光蛋白质的发现、发展和工作的重要意义.类-绿色荧光蛋白质可用于在时间和空间上监视越来越多的活细胞中的现象和机制,如基因表达、蛋白质的定位和动态学等.因此可以说,绿色荧光蛋白质的发现是联系到生物科学上的一次技术革命.     

Dielectrophoretic characterization and isolation of jellyfish stinging capsules

Jellyfish stinging capsules known as nematocysts are explosive, natural‐injection systems with high potential as a natural drug‐delivery system. These organelles consist of a capsule containing a highly folded thin needle‐like tubule and a matrix highly concentrated with charged constituents that enable the tubule to fire and penetrate a target. For the purpose of using these nematocysts as drug delivery system it is first required to purify subpopulations from heterogeneous population of capsules and to investigate each subpopulation's distinct function and characteristics. Here, the nematocysts’ dielectric properties were experimentally investigated using dielectrophoretic and electrorotational spectra with best fits derived from theoretical models. The dielectric characterization adds to our understanding of the nematocysts’ structure and function and is necessary for the dielectrophoretic isolation and manipulation of populations. As expected, the effect of monovalent and divalent exchange cations resulted in higher inner conductivity for the NaCl treated capsules; this result stands in agreement with their relative higher osmotic pressure. In addition, an efficient dielectrophoretic isolation of different nematocyst subpopulations was demonstrated, paving the way to an understanding of nematocysts’ functional diversity and the development of an efficient drug delivery platform.     

An arbitrary Lagrangian–Eulerian formulation for the numerical simulation of flow patterns generated by the hydromedusa Aequorea victoria

A new geometrically conservative arbitrary Lagrangian–Eulerian (ALE) formulation is presented for the moving boundary problems in the swirl-free cylindrical coordinates. The governing equations are multiplied with the radial distance and integrated over arbitrary moving Lagrangian–Eulerian quadrilateral elements. Therefore, the continuity and the geometric conservation equations take very simple form similar to those of the Cartesian coordinates. The continuity equation is satisfied exactly within each element and a special attention is given to satisfy the geometric conservation law (GCL) at the discrete level. The equation of motion of a deforming body is solved in addition to the Navier–Stokes equations in a fully-coupled form. The mesh deformation is achieved by solving the linear elasticity equation at each time level while avoiding remeshing in order to enhance numerical robustness. The resulting algebraic linear systems are solved using an ILU(k) preconditioned GMRES method provided by the PETSc library. The present ALE method is validated for the steady and oscillatory flow around a sphere in a cylindrical tube and applied to the investigation of the flow patterns around a free-swimming hydromedusa Aequorea victoria (crystal jellyfish). The calculations for the hydromedusa indicate the shed of the opposite signed vortex rings very close to each other and the formation of large induced velocities along the line of interaction while the ring vortices moving away from the hydromedusa. In addition, the propulsion efficiency of the free-swimming hydromedusa is computed and its value is compared with values from the literature for several other species.