A New Diterpene Acid from the Flowers of Heteropappus altaicus

A new compound, named heteraltaic acid was isolated from the flowers of Heteropappus altaicus (willd) Novopokr. The compound was elucidated as (5R, 6S, 8aS) -5-[2-(3-furyl) ethyl-5, 6, 8a-trimethyl-4a, 5, 6, 7, 8, 8a -hexahydro-l-naphthalenecarboxylic acid] by the combination of 1D and 2D NMR techniques (HSQC, HMBC) and X-ray analysis.     

Seed Oil Fatty Acids of Mongolian Compositae: The trans‐Fatty Acids of Heteropappus hispidus,Asterothamnus centrali‐asiaticus and Artemisia palustris

Seed oils from the Compositae plant family are known to contain a variety of unusual fatty acids. Subsequent to the recent discovery of γ‐linolenic acid in Saussurea and Youngia, further Mongolian Compositae species were investigated for their seed oil fatty acid composition. A number of δ3trans‐fatty acids (16 : 1δ3t, 18 : 1δ3t and 18 : 3δ3t, 9c, 12c) were found in the seed oils of Heteropappus hispidus and Asterothamnus centrali‐asiaticus. The latter fatty acid, but not the trans‐monoenes, was also found in one species of Artemisia. These unusual fatty acid isomers were characterized by capillary gas‐liquid chromatographic (GLC) separations in combination with other chromatographic techniques (analytical thin layer chromatography, TLC and preparative argentation TLC), and infrared spectrocsopy (IR). Their identity was further confirmed by co‐chromatography with other seed oils known to contain these trans‐fatty acids. The fact that within the Compositae plant family there are apparently two or three distinct groups of genera containing δ3trans‐fatty acids is discussed.     

气相色谱-质谱法测定阿尔泰狗娃花挥发精油中化学成分

采用水蒸气回流法提取阿尔泰狗娃花挥中挥发精油,用气相色谱-质谱法分离和鉴定阿尔泰狗娃花挥发精油中化学成分,用归一法测定其相对含量,共分离出50种化学成分,鉴定了39种化学成分,约占总成分相对含量的84.7%。     

阿尔泰狗娃花化学成分研究

本文从阿尔泰狗娃花Heteropappus altaicus的乙醇提取物中分离出12个已知化合物,经过红外光谱(IR),质谱(MS),核磁共振谱(NMR)等分析方法及与标准品对照,鉴定了它们的结构。分别为:木栓酮(1),表木栓醇(2),达玛烷-20,24-二烯-3β-醇(3),达玛烷-20,24-二烯-3β-乙酰基(4),α-波甾醇(5),槲皮素(6),3'-甲氧基槲皮素(7),槲皮素-3-O-β-D-葡萄糖苷(8),阿魏酸(9),β-谷甾醇(10),β-胡萝卜苷(11),没食子酸乙酯(12)。其中化合物9和12为首次从该属植物中分离得到,化合物5,6,9和12为首次从该植物中分离得到。     

Bioassay-guided isolation of antioxidants from Astragalus altaicus by combination of chromatographic techniques

An efficient method for bioassay-guided preparative isolation of antioxidants from the n-butanol extract of Astragalus altaicus Bunge was ingeniously developed by combination of silica gel column chromatography and high-speed counter-current chromatography. Under the bioassay-guidance of antioxidant activities, the antioxidants were gradually separated from the crude sample of Astragalus altaicus Bunge by silica gel column chromatography and high-speed counter-current chromatography. Silica gel column chromatography separation was performed with chloroform, chloroform-methanol (100:1~5:1, v/v) and chloroform-methanol-water (5:1:0.1~2:1:0.1, v/v/v). High-speed counter-current chromatography separation was performed with a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:1:6, v/v/v), which was successfully selected by thin layer chromatography analysis, at a flow rate of 1.5 mL/min. As a result, isorhamnetin-3-gentiobioside (20.8 mg), rutin (82.0 mg), and narcissin (12.8 mg) were obtained for the first time from 200 mg of the crude sample, ABS-5 of Astragalus altaicus Bunge. The purities were all at over 95% by high-performance liquid chromatography analysis, and their structures were unambiguously identified by mass spectroscopy, (1) H, and (13) C nuclear magnetic resonance spectroscopy. Antioxidant activities of the three compounds were also assayed by in vitro ABTS [2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diamonium salt] radical cation scavenging activity. Among them, rutin possessed the highest antioxidant capacity with SC(50) value of 22.15 μg/mL.     

阿尔泰狗哇花总皂苷的提取与纯化

对阿尔泰狗哇花总皂苷的提取与纯化工艺进行了研究.利用正交设计确定了提取温度、乙醇浓度、溶剂用量、时间及次数等提取工艺参数.比较了五种大孔吸附树脂和聚酰胺树脂对阿尔泰狗哇花总皂苷的吸附与脱附性能.结果表明,在优化的提取条件下提取,经AB-8大孔吸附树脂柱层析分离纯化,制得总皂苷产品,其含量比原药材提高约7倍多,进一步完善后可适用于工业化生产.