阿魏酸钠与牛血清白蛋白结合作用的研究

用荧光、紫外光谱法研究了在模拟人体生理条件下阿魏酸钠与牛血清白蛋白(BSA)之间的相互作用,求得阿魏酸钠与BSA的结合常数K=(5.30士0.18)×105,根据热力学参数确定了阿魏酸钠与BSA之间的作用力类型,根据Foster偶极-偶极非辐射能量转移原理,计算了阿魏酸钠与BSA相互结合时其授体一受体间的距离r=1.01nm.     

Structural elucidation of new ferulic acid-containing phenolic dimers and trimers isolated from maize bran

Four new phenolic dimers and trimers that contain ferulic acid moieties were isolated from the alkaline hydrolyzate of insoluble maize bran fiber and their structures were established by 1D/2D NMR and mass spectrometry. The biological role of one dimer remains unclear whereas the dimeric vanillin-ferulic acid-cross-product probably represents an oxidative degradation product from the corresponding diferulate. Both ferulic acid dehydrotrimers are able to cross-link polysaccharide chains. However, the 5-5/8-O-4(H₂O)-triferulic acid may be a cross-link in its identified structure whereas we assume that the identified 8-O-4/8-5(non-cyclic)-triferulic acid arose from a natural 8-O-4/8-5(cyclic)-triferulate analog during the saponification process.