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1.
Applied Biochemistry and Biotechnology - 相似文献
2.
控温相变免疫分析测定雌酮 总被引:4,自引:0,他引:4
通过热引发聚合与氧化还原引发聚合,合成水凝胶,作为雌酮全抗原(E1— BSA)载体;以异硫氰基灾光素标记雌酮的单克隆抗体(McAb)作为示踪剂,建立了控温相变竞争免疫分析测定雌酮(EI)的方法并应用于分析血清样品。实验表明:以热引发聚合合成的水凝胶为载体的荧光免疫分析方法具有更高的灵敏度,它的线性范围为10-700μg/L,检出限为1μg/L(n=10,3倍空白)。经水凝胶性质的比较(包括LCST、非特异性吸附、抗原与抗体反应时间及固定化抗原活性),证明了热引发聚合的水凝胶较氧化还原引发聚合的水凝胶更适合于作为免疫分析的载体。 相似文献
3.
4.
Sol-gel entrapment of monoclonal anti-atrazine antibodies 总被引:1,自引:0,他引:1
A. Turniansky D. Avnir A. Bronshtein N. Aharonson M. Altstein 《Journal of Sol-Gel Science and Technology》1996,7(1-2):135-143
We report the successful doping of a sol-gel matrix with an antibody, retaining its ability to bind free antigen from an aqueous solution. The particular system described is monoclonal anti-atrazine mouse antibody which was entrapped in SiO2 sol-gel matrices, prepared from tetramethoxysilane by several methods. Atrazine was selected as a model compound for this study, within the framework of the development of immunochemical-based methods for monitoring pesticide residues and other organo-synthetic environmental contaminants. Nanogram quantities of atrazine were applied on SiO2 sol-gel columns doped with this antibody, and the amount of eluted antigen was determined by Enzyme Linked ImmunoSorbent Assay (ELISA). Under appropriate sol-gel-forming conditions, high amounts of atrazine were bound to the sol-gels, ranging between 60% and 91% of the amount applied to the column. The combination of the properties of the sol-gel matrix (e.g., stability, inertness, high porosity, high surface area and optical clarity), together with the selectivity and sensitivity of the antibodies, enable extension of this feasibility study to development of a novel group of immunosensors which could be used for purification, concentration and monitoring of a variety of residues from different sources.Contribution from the Agricultural Research Organization (ARO), Bet Dagan, Israel. No., 1697-E, 1995 series. 相似文献
5.
A. Lakshmi Sailaja K. Kishore Kumar D. V. R. Ravi Kumar C. Mohan Kumar N. M. Yugandhar G. Srinubabu 《Chromatographia》2007,65(5-6):359-361
A simple, rapid, and sensitive high-performance liquid chromatographic method for estimation of efavirenz in human plasma
has been developed and validated. Chromatography was performed with C18 analytical column and 50:50 acetonitrile–phosphate buffer (pH 3.5) as mobile phase. Compounds were monitored by UV detection
at 247 nm. The retention time for efavirenz was 6.45 min and that for the internal standard, nelfinavir, was 2.042 min. Response
was a linear over the concentration range of 0.1 μg–10 μg mL−1 in human plasma. The method was simple, specific, precise and accurate and was useful for bioequivalence and pharmacokinetic
studies of efavirenz. 相似文献
6.
A simple direct enzyme immunoassay for semiquantitative detec tion of antibodies is suggested. It is based on the difference
in diffusion rates in a gel for a synthetic low-mol-wt antigen and of its complexes with antibodies to be detected. Sensitivity
and specificity of the devel oped assay are equal to an ELISA method. The assay has been tested with antibodies against HIV
protein gp41 in rabbit serum. Possible applications and limitations of the method are discussed. 相似文献
7.
环丙基甲基酮是合成环丙氟哌酸类广谱抗菌药物和抗艾滋特效药依法韦仑的重要中间体,在医药、化工及农林等领域均有广泛应用。环丙基甲基酮的合成方法大致分为4类,分别以酮类、酯类、醇类及呋喃有机物为原料,在一定条件下生成环丙基甲基酮粗品,经萃取及精馏得到环丙基甲基酮纯品。本文综述了环丙基甲基酮的合成研究进展。 相似文献
8.
Alexandra E. BotchkarevaFabiana Fini Sergei EreminJosep V. Mercader Angel MontoyaStefano Girotti 《Analytica chimica acta》2002,453(1):43-52
A heterogeneous chemiluminescent (CL) flow immunoassay for DDT was optimized comparing different types of immunoaffinity supports: beads, nylon coils and membranes (membranes HyBondN+). In order to characterize solid immunoaffinity supports two basic immunoassay formats were performed, using (1) enzyme-labeled secondary and (2) enzyme-labeled specific monoclonal antibodies (MAbs). In both formats, hapten DDT5 conjugated to ovalbumin immobilized on solid supports according to the appropriate immobilization procedure, enzyme label (horseradish peroxidase, HRP) and luminescent detection (luminol/H2O2/p-iodophenol) were used. The lowest limit of detection (LOD), 1 nM p,p-DDT, was obtained with a membrane-based flow immunoassay with HRP-labeled specific antibody. Beads and packed tubing were discarded as appropriate supports because of the difficulties encountered for reproducible packing and the occurrence of light scatterring (beads), which seriously compromised the performance and reproducibility of the flow immunoassay. 相似文献
9.
电化学免疫分析法研究进展 总被引:27,自引:8,他引:27
电化学免疫分析法是将免疫分析与电化学分析技术相结合的一种免疫分析新方法,近十多年来,电化免疫分析的研究有了迅速的发展。本文对电化学的免疫分析法的标记物、免疫方法、电化学检测技术进行了概括总结,并展望了电化学免疫分析的发展前景。 相似文献
10.
应用活化鲁米诺,用优化的增强化学发光酶联免疫分析体系测定人绒毛膜促性腺激素,检测限为0.2mIU/ml。线性范围0~200mIU/ml,与放射免分析测定结果比较,相关性良好。进而又发展了一种半定量的照相测定法,通过实际血清样品测定,效果良好。 相似文献