二氯荧光素-氯化十六烷基吡啶荧光猝灭法测定微量碘

本文研究了用二氯荧光素(DCF)-氯化十六烷基毗啶(CPC)体系荧光猝灭法测定微量碘。在pH为2.8～3.1的H_2SO_4介质中,检出限为0.30μg/25ml,线性范围为0.30～2.00μg/25ml。本法灵敏度高,选择性好,适用于微量碘的测定。     

On-Line Flow-Injection Chemiluminescence Determination of Bovine Serum Albumin Using Surfactant-Enhanced Dichlorofluorescein-Hypochlorite System

A flow injection technique combined with a chemiluminescence method was established for the determination of bovine serum albumin (BSA). Strong chemiluminescence was observed when BSA-dichlorofluoresce (DCF) complex was oxidized by sodium hypochlorite (NaClO) in an alkaline medium and in the presence of cetyltrimethylammonium bromide (CTAB). The reaction conditions of the chemiluminescence were carefully optimized. Under the optimal conditions, the method had a linear range of 0.01–20.0 μg/mL, with a detection limit of 0.007 μg/mL for BSA (3σ). The relative standard deviation of 1.0 μg/mL BSA (n = 8) is 1.4%. The method was applied to determine BSA in milk samples and it worked well.     

过氧化氢氧化二氯荧光素动力学荧光法测定痕量铜

研究了在氨性介质中，铜(Ⅱ)催化过氧化氢氧化二氯荧光素的褪色反应，建立了动力学荧光法测定痕量铜的新方法。方法的检出限为0．06μg/L，测定铜的线性范围为0．2～4．0μg/L。已用于人发、茶叶和自来水中痕量铜的测定。     

一些含氮有机物在N-氯代丁二酰亚胺-二氯荧光素体系中的后化学发光反应

研究了53种含氮有机物在N-氯代丁二酰亚胺(NCS)-二氯荧光素体系中的后化学发光行为,结果发现,很多物质在NCS-二氯荧光素体系中都具有后化学发光活性.经过结构分析发现,这类物质有无后化学发光活性以及后化学发光活性的强弱主要与其分子结构中N原子的状态有关.在对有关反应的动力学性质、化学发光光谱及有关物质荧光光谱的研究基础上,提出了这类后化学发光反应的可能机理.     

铁氰化钾-二氯荧光素-甲基多巴化学发光体系的研究

实验发现,铁氰化钾在碱性条件下能氧化甲基多巴产生微弱的化学发光,而二氯荧光素能大大增强该体系的发光强度。基于此,并结合流动注射技术,建立起一种直接测定甲基多巴的流动注射化学发光新方法。该方法的检出限为5.8×10-9g/mL,甲基多巴在1.0×10-8~1.0×10-6g/mL范围内与发光强度呈良好的线性关系。对1.0×10-7g/mL的甲基多巴平行测定11次,其相对标准偏差为2.9%。用该法对甲基多巴片剂含量进行了测定,结果令人满意。     

N-溴代琥珀酰亚胺-二氯荧光素化学发光体系测定废水中5-磺基水杨酸

建立了测定5-磺基水杨酸的流动注射化学发光新方法。 研究了影响化学发光强度的因素,并初步探讨了可能的发光机理。 在最佳化学发光条件下,化学发光强度与5-磺基水杨酸浓度在5.0×10-8~2.0×10-5 mol/L范围内呈良好的线性关系,检出限为1.0×10-8 mol/L,对5.0×10-6 mol/L的5-磺基水杨酸平行测定9次,其相对标准偏差为2.7%。 该法可用于强力霉素废水中5-磺基水杨酸的测定。     

卤代荧光素荧光猝灭法测定肾上腺色腙

在pH=6.6 的缓冲介质中, 肾上腺色腙(CBZC)与二氯荧光素(DCF)、二溴荧光素(DBF) 和二碘荧光素(DIF)通过静电引力、芳基堆积作用和范德华力形成摩尔比为2: 1的复合物, 引起上述二卤代荧光素的荧光发生猝灭, 最大猝灭波长分别位于533, 536和560 nm. 其荧光猝灭值(ΔF)在一定范围内与肾上腺色腙浓度成正比, 荧光猝灭反应具有较高的灵敏度, 对CBZC的检出限分别为3.3 ng/mL(CBZC-DCF体系), 5.7 ng/mL(CBZC-DBF体系)和129.6 ng/mL(CBZC-DIF体系). 考察了共存物质的影响、荧光猝灭反应的适宜条件和影响因素, 结果表明, 该方法具有良好的选择性, 可用于CBZC的血药和尿药浓度的快速检测. 从温度的影响、荧光寿命以及Stern-Volmer图判断该反应为静态猝灭反应.     

An ultrasensitive post chemiluminescence reaction of ammonium in NBS-dichlorofluorescein system and its application

A strong post chemiluminescence (PCL) phenomenon was observed when ammonium was injected into the reaction mixture after the finish of CL reaction of N-bromosuccinimide (NBS) and dichlorofluorescein. Based on this, a sensitive flow injection PCL method was established for the determination of ammonium. The possible CL mechanism of the reaction was proposed based on a series of experiments. The PCL intensity responded linearly to the concentration of ammonium in the range 3.0 × 10⁻¹¹-1.0 × 10⁻⁷ g mL⁻¹ with a detection limit of 1 × 10⁻¹¹ g mL⁻¹. The relative standard deviation (R.S.D.) was 1.4% for 1.0 × 10⁻⁹ g mL⁻¹ ammonium (n = 11). This method had been applied to the determination of ammonium in samples of mineral water, tap water and river water.     

Chemiluminescence Flow Injection Determination of Copper (II)

ABSTRACT

A sensitive and selective flow injection chemiluminescence method for the determination of copper over the range of 0.5-7.5 ng is described. The method is based on quenching effect of copper (II) on the chemiluminescent reaction of dichlorofluorescein and hydrogen peroxide in alkaline media. Method development includes optimization of reagent concentrations and flow conditions. The optimized method yielded a detection limit (3σ) of 0.2 ng. The method is simple, fast, selective and precise and was used for the determination of copper in blood sera.