排序方式: 共有17条查询结果,搜索用时 17 毫秒
1.
采用柱切换色谱-离子阱质谱联用法研究复方丹参滴丸中丹参素及其代谢产物 总被引:10,自引:0,他引:10
采用柱切换高效液相色谱技术对人血清中的丹参素及其代谢产物进行富集和分离,利用对电喷雾离子阱多级质谱负离子模式进行结构分析。一级流动相为甲醇-水(30:70,V/V),流速0.8mL/min;二级流动相为甲醇-水-甲酸铵(15:85:0.5,V/V/V),流速0.2mL/min;在人血清中检测到丹参素及其代谢产物,并探讨了主要代谢产物乙酰化的丹参素(m/z 240.2)。建立一种复方丹参滴丸中丹参素及其代谢产物在人体内代谢过程的高效分析方法。该方法可用于复方丹参滴丸中丹参素及与其结构类似的其它药物的体内代谢研究。 相似文献
2.
A simple, accurate and effective micellar electrokinetic capillary chromatography (MEKC) method for the separation and determination
of protocatechuic aldehyde (PA), danshensu (DSS), salvianolic acid B (SAB) and gallic acid (GA) in Chinese medicine 'SHUANGDAN'
granule was developed in this work. The separation was carried out in a 50.0 cm (42.0 cm to the detector) × 75 μm i.d. fused-silica
capillary using the optimum buffer solution containing 5.0 mM borate, 15 mM phosphate, 35 mM SDS and 10% (v/v) acetonitrile. The linear relationships between the concentration of the analytes and the corresponding peak areas were investigated,
and excellent linear behavior over the investigated concentration ranges was revealed (R2: 0.9985 for PA, 0.9994 for DSS, 0.9989 for SAB and 0.9991 for GA, respectively). The proposed method was successfully applied
to the determination of these four active components contained in 'SHUANGDAN' granule. The recoveries of these four components
were 97.6–101.2%, 97.6–100.5%, 96.9–101.6% and 97.6–102.9%, respectively. 相似文献
3.
毛细管胶束电动色谱法测定复方丹参滴丸中三种水溶性成份 总被引:3,自引:0,他引:3
在胶束电动色谱模式下考察了影响原儿茶醛、丹参素和原儿茶酸分离的重要因素并优化了关键的分离条件,建立了一种简单快速的利用毛细管胶束电动色谱DAD检测器分离分析原儿茶醛、丹参素、原儿茶酸的新方法.当缓冲溶液为50 mmol/L磷酸盐缓冲液(pH 7.2)和20 mmol/L SDS,分离电压为15 kV和分离温度为25℃时,三种分析物在10 min内实现了基线分离.结果表明,所建立的分离方法的线性范围宽,检出限低,重现性好.并利用该方法测定了复方丹参滴丸中的原儿茶醛和丹参素. 相似文献
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5.
A simple, sensitive, and accurate liquid-chromatographic method with UV detection has been developed for simultaneous determination
of spinosin, danshensu, and protocatechuic aldehyde in traditional Chinese Zaoren-an-shen capsules. Chromatographic separation
was achieved on a C18 column. Vanillin was used as internal standard and the UV detection wavelength was 280 nm. Excellent linear behavior was
observed over the concentration ranges investigated, with R values higher than 0.9997 for all the analytes. The limits of quantification for spinosin, danshensu, and protocatechuic
aldehyde were 1.31, 0.74, and 0.27 μg mL−1, respectively. This validated method can be used for routine quality control of Zaoren-an-shen capsules. 相似文献
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7.
丹参素自氧化与促氧化机理 总被引:2,自引:0,他引:2
采用过氧化氢电极与电子自旋共振(ESR)技术对不同pH值条件下丹参素的自氧化及Cu2+对丹参素的促氧化反应机理进行了研究.实验结果发现:在碱性条件下(pH=8.5),丹参素自氧化作用异常显著,氧化首先生成O2-·,随后可歧化为H2O2,并且Cu2+对H2O2信号增强也起着显著的促进作用;在弱酸性条件下(pH=6.5),丹参素自氧化过程受到抑制,主要表现为少许Cu2+的促氧化作用;而在生理条件下(pH=7.4),丹参素的自氧化过程缓慢,有少量H2O2生成,并且Cu2+的促氧化作用非常有限.因此,当丹参素作为一种高效抗氧化剂药用时,其自身的自氧化与金属离子促氧化作用都应有所考虑,尤其在偏碱性体液中,更易产生大量的超氧阴离子自由基,并对生物机体造成某种损害. 相似文献
8.
丹参活血化瘀活性成分的靶标 总被引:5,自引:0,他引:5
丹参活血化瘀活性成分主要有丹参素、迷迭香酸、丹参酮等化合物,这些化合物具有某些共同的化学结构特征.运用靶分子信息学平台,实现丹参共同化学结构特征与已知结构的蛋白质的结合能力的系统化研究,并进一步探索丹参活血作用的机理.结果表明,丹参活血化瘀活性成分主要通过调控人体内肾上腺素类激素的代谢网络,在一定程度上抑制肾上腺素类激素的分解代谢,形成积累,从而对心血管疾病具有治疗效果.丹参活血化瘀活性成分的主要靶标是苯乙醇胺N-甲基转移酶、儿茶酚O-甲基转移酶和单胺氧化酶A. 相似文献
9.
《Analytical letters》2012,45(5):737-756
Abstract A liquid chromatography‐tandem mass spectrometry was developed and validated for the simultaneous determination of protocatechuic aldehyde, danshensu, salvianolic acid B, and hydroxysafflor yellow A, with rutin as internal standard. Electrospray ionization patterns and efficiency of the analytes, along with their fragmentation, were investigated to achieve sensitive electrospray tandem mass spectrometry (ESI‐MS/MS) detection. Plasma samples were extracted by solid‐phase extraction columns, and the analytes were separated on a Dikma Diamonsil C18 (200 mm×4.6 mm i.d., 5 µm) column using a mobile phase comprised of methanol:acetonitrile: 0.5% formic acid (20∶25∶55, v/v/v) at a flow rate of 1 ml/min. Detection was performed on a Finnigan TSQ ripple quadrupole tandem mass spectrometer in negative ion‐selected monitoring mode using electrospray ionization. Good linearity over the range 10–1000 ng/ml for danshensu, salvianolic acid B, and hydroxysafflor yellow A, and 5–500 ng/ml for protocatechuic aldehyde with acceptable accuracy and precision, respectively. All the validation data, such as accuracy, precision, and stability, were within the required limits. It was a potential platform for the pharmacokinetic and absorption, distribution, metabolism, and excretion (ADME) study of multiple constituent traditional Chinese medicine. 相似文献
10.
(+)-Salvianolic acid A, one of the most active components in the traditional Chinese medicine Danshen, has been synthesized over 10 steps in 6.5% overall yield. Starting from inexpensive ortho-vanillin and sodium Danshensu (synthesized via asymmetric catalysis in our group), the process consists of the following: A Wittig reaction that gives the desire product with absolute E-configuration, a demethylation reaction with AlCl3 in a satisfactory yield, and a practical deprotection of allylic groups to afford the terminal product (+)-salvianolic acid A. The current synthetic technology possesses the advantages of using inexpensive starting materials, mild reaction conditions and has the potential for use in large scale synthesis. 相似文献