Cladosporium cladosporioides发酵物中的新的化学成分

在生物活性的导向下,我们对Cladosporium cladosporioides菌种的发酵液的化学成分进行了研究,从乙酸乙酯萃取物中共分离得到了4个枝孢菌素类化合物.应用光谱分析方法(尤其是1D、2D NMR及MS)结合文献数据,确定了它们的结构,其中化合物1-3为新的化合物,命名为Cladosporol A-C,化合物4为已知化合物Cladosporol.活性测试结果表明,化合物1-4对Staphylococcus aureus有较强的抑制作用.文献报道该类化合物还是很强的β-1,3-葡聚糖生物合成抑制剂.     

Halogenated cladosporols produced by the sodium halide-supplemented fermentation of the plant-associated fungus Cladosporium sp. TMPU1621

A new cladosporol derivative, 2-chloro-cladosporol D (6), was isolated together with known cladosporol (1), cladosporols B–D (2–4), and cladosporol F (5) from the culture broth of plant-associated Cladosporium sp. TMPU1621. The structure of 6 was elucidated from spectroscopic analyses including NMR and MS data. The productivity of 6 increased in 3% NaCl-supplemented medium; therefore, fermentation of the producing fungus TMPU1621 with 3% NaBr was investigated, leading to the induced production of a new halogenated cladosporol, 2-bromo-cladosporol D (7). Compound 2 showed the lowest MIC values of 3.13 and 12.5?μg/mL against two MRSA strains, ATCC43300 and ATCC700698, respectively.