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Jun Liu Lingchao Cai Wei Sun Rujin Cheng Nanxi Wang Ling Jin Sharon Rozovsky Ian B. Seiple Lei Wang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(52):19015-19019
Small‐molecule crosslinkers are invaluable for probing biomolecular interactions and for crosslinking mass spectrometry. Existing chemical crosslinkers target only a small selection of amino acids, while conventional photo‐crosslinkers target almost all residues non‐specifically, complicating data analysis. Herein, we report photocaged quinone methide (PQM)‐based crosslinkers that target nine nucleophilic residues through Michael addition, including Gln, Arg, and Asn, which are inaccessible to existing chemical crosslinkers. PQM crosslinkers were used in vitro, in Escherichia coli, and in mammalian cells to crosslink dimeric proteins and endogenous membrane receptors. The heterobifunctional crosslinker NHQM could crosslink proteins to DNA, for which few crosslinkers exist. The photoactivatable reactivity of these crosslinkers and their ability to target multiple amino acids will enhance the use of chemical crosslinking for studies of protein–protein and protein–DNA networks and for structural biology. 相似文献
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Zhen‐Gao Feng Wen‐Ju Bai Thomas R. R. Pettus 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2015,127(6):1884-1887
The total syntheses of medicarpin, sophoracarpan A, and kushecarpin A from a common intermediate are achieved by using ortho‐ and para‐quinone methide chemistry. Additionally, the relative stereochemistry of sophoracarpan A and B have been reassigned. 相似文献
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