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A flow-injection system for the determination of 3-hydroxybutyrate in serum is described. 3-Hydroxybutyrate dehydrogenase is immobilized on poly(vinyl alcohol) beads and incorporated in a flow-injection system. 1-Methoxy-5-methylphenazinium methylsulphate reacts with enzymatically generated NADH to give H2O2, which is detected chemiluminometrically with the reaction of luminol and hexacynoferrate(III). Serum is diluted and filtered through an ultrafiltration membrane. The system responds linearly to injected samples (80 μl) in the concentration range 0.5–300 μM; the detection limit is 0.1 μM. The within-day relative standard deviation (n = 90) for 58 μM 3-hydroxybutyrate in serum is 0.8%. The maximum throughout is 20 samples per hour. The immobilized enzyme is stable for at least 1 month. 相似文献
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光泽精化学发光体系的研究与应用 总被引:5,自引:0,他引:5
对光泽精化学发光体系的动力学性质、荧光光谱、化学发光光谱及化学发光机理进行研究和探讨。详细研究了光泽精 - H2 O2 - Co( )流动注射化学发光体系的最佳分析条件。结果表明 ,当光泽精 (L c)为 2 .5×10 - 5mol/L、H2 O2 为 0 .5 mol/L、Na OH为 0 .4 mol/L、CTMAB为 2 .0× 10 - 5mol/L时 ,测定 Co( )的校准曲线线性范围为 0 .0 5— 2 0 0μg/m L ,检出限为 0 .0 1μg/m L。对 10μg/m L Co( )连续测定 7次 ,相对标准偏差为 1.7%。 相似文献
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林诒勋 《高校应用数学学报(英文版)》2003,18(3):361-369
§ 1 IntroductionThe cutwidth problem for graphs,as well as a class of optimal labeling and embed-ding problems,have significant applications in VLSI designs,network communicationsand other areas (see [2 ] ) .We shall follow the graph-theoretic terminology and notation of [1 ] .Let G=(V,E)be a simple graph with vertex set V,| V| =n,and edge set E.A labeling of G is a bijec-tion f:V→ { 1 ,2 ,...,n} ,which can by regarded as an embedding of G into a path Pn.Fora given labeling f of G,th… 相似文献
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Ginette Ratovo Jean‐Pierre Souchard Pascale Urizzi Yvon Coulais Franoise Nepveu Etienne Hollande 《应用有机金属化学》2004,18(1):1-8
Pancreatic cancer has an extremely poor prognosis, due, in part, to lack of methods for early diagnosis. The present study was designed to evaluate the potential of labeling low‐density lipoprotein (LDL) with a radionuclide using a lipid chelating agent, bis(stearylamide) of diethylenetriaminepentaacetic acid (L), to detect pancreatic tumors by gamma‐scintigraphy. Previous studies indicated that the difficulty of visualization of pancreatic tumors was due to their poor vascularization. This study compares the ability of two radiotracers, 111In–L–LDL and 153Gd–L–LDL to target highly vascularized rat pancreatic tumors (AR4‐2J) implanted in nude mice. Biodistribution studies showed that the tumor uptake of 111In–L–LDL and 153Gd–L–LDL tracers was twofold and fivefold higher respectively than with the controls (111In citrate and 153Gd citrate respectively). These tracers would thus be suitable for scintigraphic imaging. We show here that LDL could be employed as a delivery system for tracers such as 111In or 153Gd when these two radionuclides are complexed by a lipid‐chelating anchor, and that 111In–L–LDL and 153Gd–L–LDL enabled better visualization of the pancreatic tumor tissues, with a better result with 153Gd–L–LDL. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
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H. Hommel A. Touhami A. Halli A. P. LeGrand 《Journal of Polymer Science.Polymer Physics》1995,33(16):2189-2198
EPR spectroscopy of labeled poly(ethylene oxide) (PEO) grafted on silica has been used to characterize the conformation and local dynamics of the chains. Grafted molecules of MW 2000 with grafting ratios of 0.045, 0.057, 0.126, and 0.42 molecules/nm2 were in contact with benzene. The mobility of the label was compared with that observed for solution of PEO from very diluted to highly concentrated and even bulk PEO. Thus, the concentration inside the grafted layer could be evaluated and also the thickness, which evolves rather linearly with the grafting ratio. © 1995 John Wiley & Sons, Inc. 相似文献
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提出了一种非刚性点匹配的算法并把其运用于医学图像配准.该算法采用信号滤波的方法来获得点集间的匹配信息,并运用松弛标记法将各点邻域关系对模糊的匹配信息进行迭代获得明确的匹配关系.在此基础上,利用高斯径向基函数来描述点模式间的弹性形变,在基本的迭代框架下实现问题的求解.实验结果显示在形变程度为5%、出格点比率50%和噪声标准差为5%的情况下该算法的匹配误差能控制在0.13以下,表明了该算法的鲁棒性和有效性,较好地解决了医学非刚性形变的点匹配问题. 相似文献
10.
比较了用三碘甲状腺氨酸抗体(T3抗体)、褐藻酸钠(AS)标记T3抗体及褐藻酸钠-纳米金复合物(ASN)标记的T3抗体,在通过免疫反应结合到免疫电极表面后,引起的电极表面微环境发生改变的程度;用Fe(CN)3-/4-6为电化学探针,用循环伏安法获取金电极表面微环境改变的电流信息来检测 T3抗体,检测的线性范围为100~1 600ng·ml-1,检出限为45ng·ml-1. 相似文献