排序方式： 共有21条查询结果，搜索用时 15 毫秒
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Weiwei An Lucas S. Ryan Audrey G. Reeves Kevin J. Bruemmer Lyn Mouhaffel Jeni L. Gerberich Alexander Winters Ralph P. Mason Alexander R. Lippert 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(5):1375-1379
Azanone (HNO) is a reactive nitrogen species with pronounced biological activity and high therapeutic potential for cardiovascular dysfunction. A critical barrier to understanding the biology of HNO and furthering clinical development is the quantification and real‐time monitoring of its delivery in living systems. Herein, we describe the design and synthesis of the first chemiluminescent probe for HNO, HNOCL‐1 , which can detect HNO generated from concentrations of Angeli's salt as low as 138 nm with high selectivity based on the reaction with a phosphine group to form a self‐cleavable azaylide intermediate. We have capitalized on this high sensitivity to develop a generalizable kinetics‐based approach, which provides real‐time quantitative measurements of HNO concentration at the picomolar level. HNOCL‐1 can monitor dynamics of HNO delivery in living cells and tissues, demonstrating the versatility of this method for tracking HNO in living systems. 相似文献
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Andreas Humeny 《Chemie in Unserer Zeit》2003,37(6):380-387
The high accuracy, molecular resolution and sensitivity of matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) make it an efficient method for analysing all kinds of biomolecules including nucleic acids, proteins/peptides, carbohydrates and lipids. MALDI‐TOF‐MS based high‐throughput genotyping of genetic heterogeneities possesses the potential of becoming a routine method. MAL‐DI‐TOF‐MS can be used for the identification of proteins and posttranslational modifications. Taken together, MALDI‐TOF‐MS represents a integrated platform technology in bioanalytics and molecular medicine. 相似文献
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Egon Fahr 《Fresenius' Journal of Analytical Chemistry》1977,287(2-3):97-103
Zusammenfassung Die Möglichkeiten der Verwendung von Prozeßrechenanlagen in der Bioanalytik (Multiple-Scan-Technik, Fourier-Transformations-Technik, Dokumentation von Spektren, Identifizierung von Substanzen, Bestimmung von Substanzen in Gemischen durch Mehrkomponenten-Analyse und Präzisions-Massenbestimmung) und die Technologie solcher Systeme werden diskutiert.Vortrag auf der Europäischen Tagung für Biochemische und Instrumentelle Analyse, München, 9.–13. April 1976 相似文献
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