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1.
Xing Liu Guanwen Li Huimin Wang Nan Qin Lili Guo Xiaomin Wang Sang Shen 《Molecules (Basel, Switzerland)》2021,26(11)
The areca (Areca catechu L.) nut kernel (ANK) is a good potential protein source for its high protein content of 9.89–14.62 g/100 g and a high yield of around 300,000 tons per year in China. However, utilization of the areca nut kernel is limited. To expand the usage of ANK in pharmaceutical or foods industries, areca nut kernel globulin was extracted and angiotensin-I converting enzyme (ACE) inhibition peptides were prepared and identified using gel chromatography, reversed phase HPLC separation, UPLC-ESI-MS/MS analysis and in silico screening. Finally, a novel ACE-inhibitory heptapeptide (Ala–Pro–Lys–Ile–Glu–Glu–Val) was identified and chemically synthesized. The combination pattern between APKIEEV and ACE, and the inhibition kinetics, antihypertensive effect and endothlein-1 inhibition activity of APKIEEV were studied. The results of the molecular docking demonstrated that APKIEEV could bind to four active sites (not the key active sites) of ACE via short hydrogen bonds and demonstrated high ACE-inhibitory activity (IC50: 550.41 μmol/L). Moreover, APKIEEV exhibited a significantly lowering effect on both the systolic blood pressure and diastolic blood pressure of spontaneously hypertensive rats, and had considerable suppression ability on intracellular endothelin-1. These results highlight the potential usage of APKIEEV as ingredients of antihypertensive drugs or functional foods. 相似文献
2.
Peter Reischig Jorik Blaas Charl Botha Alberto Bravin Liisa Porra Christian Nemoz Arie Wallert Joris Dik 《Journal of synchrotron radiation》2009,16(2):310-313
One of the most fascinating objects in the Rijksmuseum (Amsterdam, The Netherlands) is an early 16th century prayer nut. This spherical wooden object measures 4 cm in diameter and consists of two hemispheres connected with a small hinge so that it can be opened. The interior of the nut holds wood carvings with scenes from the life of Christ. These miniature reliefs show an incredible degree of finish with carving details well beyond the millimetre scale. In the present paper it is shown how synchrotron‐based computer X‐ray tomography revealed the structure and fabrication method of the bead. The central part of the relief was cut from a single piece of wood, rather than assembled from multiple components, underlining the extraordinary manual dexterity of its maker. In addition, a piece of fibrous material contained in the inner structure of the bead is revealed. This may have served as a carrier for an odorous compound, which would be in line with the religious function of the prayer nut. 相似文献
3.
In this work Cu and Fe bioavailability in cashew nuts was evaluated using in vitro method. Extractions with simulated gastric and intestinal fluids and dialysis procedures were applied for this purpose. The proteins separation and quantification were performed by size exclusion chromatography (SEC) coupled on-line to ultra-violet (UV) and off-line to simultaneous multielement atomic absorption spectrometry (SIMAAS). The SEC-UV and SIMAAS profiles of the protein fractions obtained by alkaline extraction (NaOH) and precipitation with HCl indicated the presence of high and low molecular weight species in the range between >75 kDa and 9.3 kDa. Almost 83% of Cu and 78% of Fe were extracted during cashew nut digestion and 90% of both elements were dialyzed. With these results it is possible to assume that 75% of Cu and 70% of Fe present in cashew nut could be bioavailable. The SEC-UV and SIMAAS chromatographic profiles obtained after in vitro gastrointestinal digestion reveal that Cu and Fe not dialyzed can be associated to a compound of 9.2 kDa. 相似文献
4.
Kinetics of deformation and fracture of nickel–iron alloy Inconel 718 under dynamic shear loading was measured using a split
torsional Hopkinson bar facility and high-speed photography. Tubular specimens with a reduced gage length and a starter notch
were sheared at strain rates up to 6 × 103 s−1. High-speed photographs of fiducial lines scribed on the specimen surface showed the development of local strains and cracking.
This paper describes the experimental and analytical procedures, illustrates average and local plastic strain evolution, and
presents shear crack initiation times and propagation speeds. 相似文献
5.
为测定海南产槟榔、沉香及槟榔-沉香(质量比为1∶1)药对中锰的含量与生物利用性,并了解锰的健康效应,采用火焰原子吸收光谱法(FAAS)测定了槟榔、沉香原药及其水煎液中锰的含量,并用体外消化-透析法模拟人体胃肠道消化,研究了锰的生物利用性.结果表明,该法回收率为98.32%~102.40%,RSD为1.24%~1.76%... 相似文献
6.
G. Mamatha A. Varada Rajulu 《International Journal of Polymer Analysis and Characterization》2019,24(3):219-226
Cellulose/Tamarind nut powder (TNP)/Silver nanoparticles (AgNPs) nanocomposites were prepared by in situ generation of AgNPs using regeneration method, followed by solution casting method. In this, TNP was used as a reducing agent. These nanocomposites were characterized using FT-IR spectroscopy, XRD and SEM and studied their mechanical properties and antibacterial activity for medical and packing applications. The FT-IR spectral studies revealed the involvement of functional groups – Polyphenols, Flavonoids and –OH in the process of reducing the metal salts into metal nanoparticles. These nanocomposites showed good antibacterial activity against five bacteria. Improved mechanical properties with good antibacterial activities make these composites suitable for medical, food and packaging applications. 相似文献
7.
Sorel Tchewonpi Sagu Gerd Huschek Thomas Homann Harshadrai M. Rawel 《Molecules (Basel, Switzerland)》2021,26(15)
The detection and quantification of nut allergens remains a major challenge. The liquid chroma-tography tandem mass spectrometry (LC-MS/MS) is emerging as one of the most widely used methods, but sample preparation prior to the analysis is still a key issue. The objective of this work was to establish optimized protocols for extraction, tryptic digestion and LC-MS analysis of almond, cashew, hazelnut, peanut, pistachio and walnut samples. Ammonium bicar-bonate/urea extraction (Ambi/urea), SDS buffer extraction (SDS), polyvinylpolypyrroli-done (PVPP) extraction, trichloroacetic acid/acetone extraction (TCA/acetone) and chloro-form/methanol/sodium chloride precipitation (CM/NaCl) as well as the performances of con-ventional tryptic digestion and microwave-assisted breakdown were investigated. Overall, the protein extraction yields ranged from 14.9 ± 0.5 (almond extract from CM/NaCl) to 76.5 ± 1.3% (hazelnut extract from Ambi/urea). Electrophoretic profiling showed that the SDS extraction method clearly presented a high amount of extracted proteins in the range of 0–15 kDa, 15–35 kDa, 35–70 kDa and 70–250 kDa compared to the other methods. The linearity of the LC-MS methods in the range of 0 to 0.4 µg equivalent defatted nut flour was assessed and recovery of internal standards GWGG and DPLNV(d8)LKPR ranged from 80 to 120%. The identified bi-omarkers peptides were used to relatively quantifier selected allergenic protein form the inves-tigated nut samples. Considering the overall results, it can be concluded that SDS buffer allows a better protein extraction from almond, peanut and walnut samples while PVPP buffer is more appropriate for cashew, pistachio and hazelnut samples. It was also found that conventional overnight digestion is indicated for cashew, pistachio and hazelnut samples, while microwave assisted tryptic digestion is recommended for almond, hazelnut and peanut extracts. 相似文献
8.
There have been many studies on the development biodegradable films using starch isolated from various food sources as a substitute for synthetic plastic packaging films. In this study, starch was extracted from ginkgo (Ginkgo biloba) nuts, which were mainly discarded and considered an environment hazard. The prepared starch (GBS) was then used for the preparation of antioxidant films by incorporating various amounts of cinnamon (Cinnamomum zeylanicum) essential oil (CZEO), which provides antioxidant activity. The prepared GBS films with CZEO were characterized by measuring physical, optical, and thermal properties, along with antioxidant activity (ABTS, DPPH, and FRAP) measurements. With the increasing amount of CZEO, the flexibility and antioxidant activities of the GBS films increased proportionally, whereas the tensile strength of the films decreased. The added CZEO also increased the water vapor permeability of the GBS films, and the microstructure of the GBS films was homogeneous overall. Therefore, the obtained results indicate that the developed GBS films containing CZEO are applicable as antioxidant food packaging. 相似文献
9.
Miarintsoa Michaele Ranarijaona Ny Aina Harivony Rambala Rakotomena Mbolatiana Tovo Andrianjafy Fenia Diane Ramiharimanana Lydia Clarisse Herinirina Niry Hasinandrianina Ramarosandratana Benoit Briou Pauline Fajardie Patrick Mavingui Estelle Mtay Voahangy Vestalys Ramanandraibe Marc Lemaire 《Molecules (Basel, Switzerland)》2021,26(24)
This article describes a part of the results obtained from the cooperation between the University of Lyon1 (France) and the University of Antananarivo (Madagascar). It shows (among others) that useful research can be carried out in developing countries of the tropics if their social, technical, and economic conditions are taken into account. The concepts and methods associated with so-called “green chemistry” are particularly appropriated for this purpose. To illustrate this approach, two examples are shown. The first deals with industrial ecology and concerns waste transformation from the production of cashew nut into an amphiphilic product, oxyacetic derivatives. This product was obtained with a high yield and in a single step reaction. It exhibited an important surfactant property similar to those of the main fossil-based ones but with a much lower ecological impact. The second talks about chemical ecology as an alternative to insecticides and used to control dangerous mosquito populations. New substituted chromones were synthesized and showed biological activities toward Aedes albopictus mosquito species. Strong repellent properties were recorded for some alkoxylated products if others had a significant attractant effect (Kairomone) depending on their stereochemistry and the length of the alkyl chain. 相似文献
10.