Chemical Constituents of Ligusticum sinensis Oliv.

The new phenylpropanoid diglycoside ligusinenoside A ( 1 ), and the two new 8,4′‐oxyneolignan(‘8‐O‐4′‐neolignan’) diglycosides ligusinenosides B ( 2 ) and C ( 3 ), together with nine known compounds, were isolated from the rhizomes of Ligusticum sinensis Oliv. The structures of 1 – 3 were elucidated on the basis of spectroscopic analyses.     

Three novel diepoxy tetrahydrochromones from agarwood artificially produced by intentional wounding

Three novel diepoxy tetrahydrochromones, oxidoagarochromones A (1), B (2), and C (3), were isolated from agarwood artificially produced by intentional wounding of Aquilaria crassna. Inductive production of these compounds was also confirmed at the early stage of wounding in A. sinensis and A. crassna. These diepoxy tetrahydrochromones would play an important role in understanding the biosynthesis of chromone derivatives in agarwood.     

密闭微波辅助萃取当归粉中的阿魏酸

利用密闭微波样品制备系统(具有压力控制部件),对当归中的有效成分阿魏酸的萃取进行研究,比较了密闭微波萃取同超声波萃取和索氏萃取阿魏酸提取率的差异.结果表明:微波萃取与其他两种萃取方法相比,具有速度快和萃取率高的特点.并得到了阿魏酸提取的最佳条件:微波功率为400 W,萃取剂为90%的乙醇(体积分数),固-液比为1∶15,微波辐射为240 s,当归粒径为75μm.     

A New Dimeric Phthalide from Angelica sinensis

Angelica sinensis (Oliv.) Diels (Umbelliferae), known as Danggui in Chinese, one of the most important traditional Chinese medicines, is used for tonifying blood and treating female irregular menstruation and amenorrhoea. It is also used for treatment of …     

毛细管区带电泳法测定冬虫夏草中的腺苷、腺嘌呤和尿嘧啶

建立了毛细管区带电泳法测定天然和人工冬虫夏草中腺苷、腺嘌呤和尿嘧啶含量的分析方法。采用15mmol/L硼砂＋14mmol/L磷酸二氢钠＋5％（V／V）甲醇（pH＝9.5）作为缓冲体系，在电压为18kV和检测波长为254nm的条件下，冬虫夏草提取液中的腺苷、腺嘌呤和尿嘧啶实现了基线分离。定量分析表明，3种成分的校正峰面积与质量浓度呈较好的线性关系(r≥0.9991）。考察了缓冲溶液的pH值、浓度及有机改性剂对腺苷、腺嘌呤和尿嘧啶迁移行为的影响。     

Simultaneous determination of eight components in Angelica sinensis based on UHPLC‐ESI‐MS/MS method for quality evaluation

A method employing ultra‐high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) for determination of eight components including ferulic acid, senkyunolide A, butylphthalide, ligustilide, butylidenephalide, senkyunolide I, senkyunolide H and levistolide A in Angelica sinensis was established. The separation was carried out using a Waters ACQUITY UHPLC BEH C₁₈ column with gradient elution with 0.1% formic acid aqueous and acetonitrile at a flow rate of 0.4 mL/min. Good linearity was attained with R² of 0.9983–0.9998 in wide concentration ranges. The method had limit of detection (LOD) and quantitation (LOQ) in the range of 0.42–6.98 ng/mL and 1.39–23.28 ng/mL, respectively. Intra‐ and inter‐day precisions varied with relative standard deviations (RSDs) from 0.33% to 0.88% and 0.37% to 1.04%, respectively. Moreover, the average recoveries were in a satisfactory range of 92.7%–102.1% with RSDs of less than 3.60%. Finally, the method was successfully applied to analyze 19 batches of A. sinensis samples grown in Min County, Gansu province, China, as well as that collected in other regions. The findings indicated that the established method is reliable and may thus be applied as a powerful tool for qualitative and quantitative analysis of components in A. sinensis, which has its implications in quality control of A. sinensis.     

Benzophenone glycosides from the pericarps of Aquilaria yunnanensis S. C. Huang

Abstract

Two new benzophenone glycosides, aquilarisides A (1) and B (2), together with six known analogues (3-8) were isolated from the pericarps of Aquilaria yunnanensis S. C. Huang. Their structures were elucidated on the basis of 1D and 2D NMR and mass spectroscopic analyses, and the absolute configuration of compound 1 was determined by experimental and calculated electronic circular dichroism (ECD) spectra. Anti-inflammatory activities of all compounds 1–8 were evaluated for their inhibitory activities against lipopolysaccharide (LPS)-stimulated induced nitric oxide (NO) production in RAW 264.7 cells using the Griess assay. Compound 2 indicated a weak inhibition of NO production.     

Changes in Physicochemical,Free Radical Activity,Total Phenolic and Sensory Properties of Orange (Citrus sinensis L.) Juice Fortified with Different Oleaster (Elaeagnus angustifolia L.) Extracts

In Iran and other parts of Western Asia, the oleaster (Elaeagnus angustifolia L.) fruit is processed in the dried powdery form, and in recent times, increasingly applied/sprinkled in fruit juices such as those made from oranges (Citrus sinensis L.). To our best knowledge, the effectiveness of oleaster fruit extract in fortifying the orange juice has not yet been reported and the knowledge of this will greatly benefit the consumers, particularly those around the Western Asia region. This current work, therefore, investigated the changes in physicochemical, free radical activity, total phenolic compounds, and sensory properties of orange juice fortified with different oleaster fruit extracts. The orange juice mix formulation comprised different concentrations (5, 10, 15, 20, and 25%) of oleaster (alcoholic, aqueous, and hydro-alcoholic) extracts. The control comprised orange concentrate (4% w/v), sugar (8.5% w/v), and citric acid (0.1% w/v) brought to the desirable volume with water. As the free radical activity depicted the antioxidant properties, the physicochemical aspects of this work involved the determinations of Brix, density, ash, pH, total acidity, sucrose, and total sugar, whereas the sensory aspects involved the determinations of color and taste. Whilst the aqueous oleaster 20 and 25% extracts produced notable physicochemical differences in the orange juice mix, both free radical activity, and phenolic compounds significantly increased (p < 0.05) after 30 days despite resembling (p > 0.05) those of control at day 1. More so, the increases in aqueous, alcoholic, and hydro-alcoholic oleaster extracts would decrease (p < 0.05) the sensory color and taste of the orange juice mix in this study.     

高效液相色谱法测定地顶孢霉培养物中的腺苷

建立测定虫草源饲料添加剂地顶孢霉培养物中腺苷含量的高效液相色谱方法。样品经过研碎、超声处理,离心、过滤后上机测定。腺苷的最佳提取条件:以超纯水为浸提液,用超声波浸提,浸提温度为40℃,浸提时间为55 min。使用Waters Spherisorb ODS2柱(150 mm×3.9 mm,5μm),以甲醇-0.01 mol/L磷酸二氢钾混合液(10∶90)为流动相,流量为1.0 mL/min,进样体积为20μL,检测波长为254 nm。腺苷的质量浓度在0.5~100μg/mL范围内与色谱峰面积成良好的线性关系,相关系数为0.9990。样品测定结果的相对标准偏差为1.65%(n=6),3水平加标的平均回收率为98.19%。该方法简便、快捷,可准确测定虫草饲料添加剂地顶孢霉培养物中腺苷的含量。     

虫草氨基酸的人工神经网络-近红外光谱快速测定方法

提出了用近红外漫反射光谱技术快速检测发酵冬虫夏草中氨基酸含量的新方法。采用比色法测定虫草菌粉中氨基酸含量。用BP神经网络建立了近红外光谱数据与氨基酸、精氨酸和总氨酸含量间的定量关联模型。通过比较不同的光谱预处理方法及光谱范围, 得到最优模型,即在7 501.7～6 097.8,5 453.7～4 246.5 cm-1区域内,近红外光谱的一阶微分光谱与其氨基酸含量之间建立模型。甘氨酸、精氨酸和总氨基酸的预测标准偏差分别为0.08,0.07和0.36,均优于主成分回归(PCR)和偏最小二乘回归(PLS)等线性模型的处理结果。结果表明,该方法是一种有效实用的非线性校正方法。为近红外光谱快速测定中药组分含量提供了一条新途径。