排序方式: 共有14条查询结果,搜索用时 31 毫秒
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优化分离与鉴定蓝斑背肛海兔口腔神经节蛋白质组 总被引:3,自引:0,他引:3
采用双向凝胶电泳技术优化分离蓝斑背肛海兔(Notarcus leachii cirrosus Stimpson, NLCS)口腔神经节(Buccal Ganglion, BG)蛋白质组, 并获得约300个蛋白质斑点. 用组合基质辅助激光解吸电离化飞行时间(MALDI-TOF)质谱技术和胶内酶解技术测定BG蛋白质组中的96个蛋白质斑点的肽指纹(Peptide mass fingerprint, PMF)图谱. 经数据库检索与比对后, 发现96种蛋白质中仅有4种蛋白质可获得较高的匹配率, 它们分别是微管蛋白(Tubulin)、 肌动蛋白(Actin)和两个1,5-二磷酸核酮糖-羧化酶/加氧酶(Ribulose-1,5-bisphosphate carboxylase/oxygenase, Ribulose), 均属于神经细胞骨架蛋白质; 同时还发现一种交配信号肽前体(Peptide mating pheromone precursor). 利用LOCtrees软件和分类法对56种蛋白质进行亚细胞定位与分类. 相似文献
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The compound 2,10-dibromo-3-chloro-8-hydroxy-β-chamigrene was analysed in detail by NMR Spectroscopy. the complete assignment of the signals in the 1H and 13C NMR spectra and the determination of the relative configurations were achieved by 2D NMR techniques, AM1 data and 1H spectrum simulation. Comparisons of the results with related spiro chamigrene systems are also presented. 相似文献
3.
A group of glycosphingolipids found in an invertebrate: Their structures and biological significance
Mei SATAKE Eishichi MIYAMOTO 《Proceedings of the Japan Academy. Series B, Physical and biological sciences》2012,88(9):509-517
A novel group of glycosphingolipids was identified in the nervous tissue and skin of the mollusc, Aplysia kurodai, which lacks gangliosides. More than 30 glycolipids were detected on HPTLC plates and the structures of 9 major glycolipids were determined. They were pentaosylglycosphingolipids and their common core structure was GalNAcα1→3Galβ1→4Glcβ1→1ceramide, except for one glycolipid in which Galβ of the core structure was replaced by Galα. 3-O-MeGalβ or 4-O-MeGlcNAcα or 3,4-O-carboxyethylideneGalβ was at their non-reducing ends. Galα or Fucα binds to Gal of the core structure at 2C as a side chain sugar. One to three 2-aminoethylphosphonic acids and/or phosphoethanolamine link to the glycolipids. Immunohistochemically, glycolipids having carboxyethylideneGal at their non-reducing ends were localized exclusively in nerve bundles. Glycolipids activated cAMP-dependent protein kinase in the rat brain and may directly activate cAMP-dependent protein kinase in a manner similar, but not identical, to that of cAMP. The biological functions of glycolipids may share neurobiological functions proposed for gangliosides in vertebrates. 相似文献
4.
Maria Kladi Dimitrios Ntountaniotis Maria Zervou Constantinos Vagias Efstathia Ioannou Vassilios Roussis 《Tetrahedron letters》2014
Glandulaurencianols A and B were isolated from the organic extract of the red alga, Laurencia glandulifera, collected from the island of Crete in South Greece. Investigation of the mollusk, Aplysia punctata, collected from the coast of Nea Makri, Central Greece, resulted in the isolation of glandulaurencianols A and C. The structures of the new metabolites, as well as their relative configurations, were established on the basis of thorough analyses of their spectroscopic data. 相似文献
5.
Lyakhova E. G. Fedorov S. N. Shubina L. K. Radchenko O. S. Kalinovsky A. I. Dvitrenok P. S. Stonik V. A. 《Russian Chemical Bulletin》2003,52(4):1022-1026
The structures of products obtained by reductive debromination and CF3COOH- and KOH-induced transformations of natural chamigrane-type sesquiterpenoid (6S,10R)-10-bromo-3,11,11-trimethyl-7-methylidenespiro[5,5]undec-2-en-4-one (dactylone) isolated from the sea hare Aplysia dactylomela were analyzed. The absolute configurations of the reaction products were established by CD spectra taking into account the configuration of the starting dactylone. 相似文献
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在镉盐胁迫下用蛋白质组学技术筛选与鉴定海兔亚口腔神经节的差异蛋白质 总被引:5,自引:1,他引:4
以蓝斑背肛海兔(Notarcus leachii cirrosus Stimpson, NLCS)的口腔神经节(Buccal ganglion, BG)为研究对象, 按BG形态对称性, 解剖成亚BG(sub-BG, SBG), 并分为左SBG和右SBG, 简称为LSBG和RSBG. 用双向凝胶电泳(2D-PAGE)技术优化分离LSBG和RSBG全蛋白质, 并采用蛋白质组学和数据库比对技术筛选与鉴定差异蛋白质. 实验结果表明, LSBG和RSBG之间的差异蛋白质主要由活性多肽的前体蛋白或降解后大片段多肽组成, 它们对维持BG的生理功能起着重要的作用. 在急性镉盐(10 μg/mL)胁迫下, NLCS的LSBG和RSBG表达了由镉盐诱导的差异蛋白质, 并采用蛋白质组学技术分别分离、筛选和鉴定, 其主要的差异蛋白质有下调的肌球蛋白、钙结合蛋白、上调的热休克蛋白和硫氧还蛋白. 这些蛋白质可能与BG细胞抗镉毒性有关, 部分差异蛋白质适合于监测镉盐污染且开展毒理学研究的蛋白指示物. 相似文献
8.
Michiya Kamio Cynthia E. Kicklighter Linh Nguyen Markus W. Germann Charles D. Derby 《Helvetica chimica acta》2011,94(6):1012-1018
Three new mycosporine‐like amino acids (MAAs), aplysiapalythines A, B, and C ( 1, 2 , and 3 , resp.) were isolated from opaline, a glandular component of the defensive ink secretion of sea hares (Aplysia californica) collected from waters off southern California. Here, we report the structure of these MAAs determined by mass spectrometry and NMR data. These new MAAs are structurally related to two known MAAs that are also present in sea hare opaline, i.e., asterina 330 ( 4 ) and palythine ( 5 ), and for which we also provide detailed data here for comparison. The fact that three of the five MAAs that we identified from sea hare opaline are novel molecules is interesting given that this represents a relatively large addition to the current list of known MAAs. This is likely because most researchers have identified MAAs through HPLC with UV detection, which is imprecise given similarities in UV spectra for different MAAs. Our findings suggest that there is much greater diversity of MAAs than is currently known. Results published elsewhere show that 1, 2 , and 4 are alarm cues for conspecific sea hares at natural concentrations, but 3 and 5 are not. 相似文献
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Mohamed-Elamir F. Hegazy Alaa Y. Moustfa Abou El-Hamd H. Mohamed Montaser A. Alhammady Serag Eldin I. Elbehairi Shinji Ohta Paul W. Paré 《Tetrahedron letters》2014
Two new sesquiterpenes, oculiferane (1) and epi-obtusane (2) have been isolated and identified from an acetone extract of the digestive gland of the sea hare, Aplysia oculifera. The structures were elucidated by spectroscopic analysis including HREIMS, 1H, 13C, DEPT, 1H–1H COSY, HMQC, and HMBC NMR; the relative configuration was confirmed by X-ray analysis. Compounds 1 and 2 exhibited cytotoxic activity in vitro against several human cancer cell lines with IC50 values in the low μg/ml range. 相似文献
10.
基质辅助激光解吸/电离质谱法研究海兔卵多肽内切酶组成、结构与功能 总被引:1,自引:1,他引:0
选择DEAE-52纤维素和Sephadex G-150为分离介质,采用柱层析法分离纯化海兔卵多肽酶(En-dopeptidease of Aplysiaegg,AEE)。选用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法测定AEE纯度和亚基分子量(约39kDa)。基质辅助激光解吸/电离(MALDI-TOF)质谱技术测定AEE由单类型亚基(M)组成,其m/z比值分别为12738.17、18108.79和38221.42,即分子式为[M3 ]、[M2 ]和[M ]。其中AEE分子量为38221.42Da(称为AEE39),是一种含金属锌的多肽酶。以胰岛素(INS)为探针,研究AEE39酶切INS能力,其酶切产物m/z比值为1449.51、2085.84、4080.41和4165.42。自行设计INS序列分析软件,鉴定AEE39酶切INS的位点,发现易酶切位点是Leu-X(氨基酸残基),其次是Glu-X,部分Phen-X、Asn-X和Ser-X结构也可以发生酶切。通过INS和海兔吸引素的分子结构比对后,指出海兔卵中的AEE39主要功能之一是负责酶切海兔吸引素,起着海兔之间信息交往、召唤、识别和交配的重要作用。AEE39具有酶切酸性多肽(acidic pep-tide,AP)中Leu-Leu的能力,属于一种广谱性多肽内切酶。 相似文献