铒离子及其阳离子卟啉配合物对金黄色葡萄球菌生长的抑制和刺激作用

用微量热法研究了Er³⁺、TMP{TMP=5，10，15，20-四(4-甲氧基苯基)卟啉}及其阳离子铒卟啉配合物[Er(TMP)(H₂O)₃]Cl对金黄色葡萄球菌生长作用的生物热动力学特征，求算了在Er³⁺、TMP和[Er(TMP)(H₂O)₃]Cl作用下，金黄色葡萄球菌生长的热动力学参数。实验结果表明：TMP对金黄色葡萄球菌生长的作用表现为单向的抑制作用，而Er³⁺和[Er(TMP)(H₂O)₃]Cl对金黄色葡萄球菌的生长有双向调节作用。在低浓度下，Er³⁺对金黄色葡萄球菌生长的刺激作用强于[Er(TMP)(H₂O)₃]Cl；在较高浓度下，[Er(TMP)(H₂O)₃]Cl对金黄色葡萄球菌生长的抑制作用显著强于Er³⁺。对金黄色葡萄球菌的抗菌活性为：[Er(TMP)(H₂O)₃]Cl>>TMP>Er³⁺。对相互作用机理做了初步探讨。     

1-芳酰基-3-(4-苯磺酰胺嘧啶)硫脲类化合物的合成

研究了以无水乙腈为溶剂,通过芳酰异硫氰酸酯与磺胺嘧啶的加成反应合成12个新的1-芳酰基-3-(4-苯磺酰胺嘧啶)硫脲、生物测试指出化合物对大肠杆菌、枯草杆菌、变型杆菌有很强的抑制作用.此外,1-苯酰基-3-(4-苯磺酰胺嘧啶)硫脲和1-(P-氯代苯酰基 )-3-(4-苯磺酰胺嘧啶)硫脲对金黄色葡萄球菌也有很强的抑制作用.     

钇离子及其阳离子卟啉配合物与金黄色葡萄球菌的相互作用

用LKB-2277生物活性检测系统考察了Y~(3+)，TMP及其阳离子型钇卟啉配合物 {[Y(TMP)(H_2O)_3]Cl, TMP = 5, 10, 15, 20-四（4-甲氧基苯基）卟啉}对金黄色 葡萄球菌全程代谢作用的影响，测定了Y~(3+)，TMP和[Y(TMP)(H_2O)_3]Cl对金黄 色葡萄球菌作用的产热曲线，根据产热曲线求算了金黄色葡萄球菌在Y~(3+)，TMP ，[Y(TMP)(H_2O)_3]Cl作用下生长代谢的速率常数k_1，k_2，抑制率I_1，I_2，和 半抑制浓度IC_(50)~1，IC_(50)~2等热动力学参数。实验结果表明：Y~(3+)，[Y (TMP)(H_2O)_3]Cl对金黄色葡萄球菌的生长代谢有双向调节作用，在低浓度下表现 为刺激作用，高浓度为抑制作用，而TMP对金黄色葡萄球菌的生长代谢主要为抑制 作用，在低浓度下表现为刺激作用，高浓度为抑制作用，而TMP对金黄色葡萄球菌 的生长代谢主要为抑制作用，对金黄色葡萄球菌的抑制作用[Y(TMP)(H_2O)_3]Cl > TMP > Y~(3+)。     

Ternary Solid Nano Organic/Inorganic Composite of Lanthanum with Acetic Acid and Curcumin/ Hydroxyapatite and Its Antibacterial Activity

Ternary solid complex was synthesized via hybridization of curcumin（Cur）, hydroxyapatite（HAP）, lanthanum（La） and acetic acid（HAc）. The nano-scale hybrid composite of La-Cur/HAP was directly prepared by the wet method. The morphologies and structures of the composite were characterized by scanning electron microscopy （SEM）, infrared（IR） spectroscopy, X-ray diffraction（XRD）, transmission electron microscopy（TEM） and energy dispersive X-ray spectroscopy. The antibacterial activities were tested by methods of minimum inhibitory concentration （MIC） and minimum bactericidal concentration（MBC）. The results show that the size of the La-Cur/HAP composite is less than 100 nm, and the composite exhibits strong bacteriostatic activity against Escherichia coli（E, colt） and Staphylococcus aureus（S, aureus） at low concentrations（in the range of 26-92μg/mL）. The composite can exhibit both of the bacteria and shows higher antibacterial activity against S. aureus than against E. colt. At the same time, La-Cur/HAP shows stronger antibacterial efficiency than ampicillin/HAP.     

一种含吗啉修饰的多吡啶钯(Ⅱ)配合物的合成、表征与抗金黄色葡萄球菌作用研究

由4′-[4-(4-吗啉基丁氧基)苯基]-2,2′∶6′,2″-三联吡啶合成了一种含吗啉修饰的三联吡啶钯配合物(Pd1),并通过核磁氢谱、元素分析和X射线单晶衍射对其结构进行表征。Pd1对金黄色葡萄球菌(S.aureus)表现出良好的抗菌活性(MIC=62.48μg/mL, MIC为最小抑菌浓度)。通过耐药实验发现金黄色葡萄球菌对Pd1不易产生耐药性,筛选发现Pd1与盐酸克林霉素联合作用时,可以增强金黄色葡萄球菌对盐酸克林霉素的敏感性。机制研究发现Pd1对与产生耐药性有关的生物膜有明显的抑制作用。此外,Pd1对金黄色葡萄球菌产生的毒素活性具有明显的抑制作用。     

取代喹啉类化合物抗菌活性的定量构效关系及分子设计

采用密度泛函理论(DFT)和逐步回归分析法对15种新合成的取代喹啉类化合物进行了定量构效关系(QSAR)研究. 在B3LYP/6-31G(d,p)水平上计算了取代喹啉的量子化学参数, 通过逐步多元回归分析筛选出影响抗菌活性的主要因素, 建立了定量构效关系方程, 并用留一法交叉分析了模型的稳定性及预测能力. 结果表明, C5的亲核电子密度fNC5及C9-N1的键级BC9-N1是影响喹啉类化合物抗金黄色葡萄球菌活性的主要因素, 所得模型对该类化合物抗菌活性有较好的预测效果. 同时基于QSAR研究结果设计了4个活性较高的新喹啉衍生物.     

Environmentally Benign Synthesis of 2-Pyrazolines and Cyclohexenones Incorporating Naphthalene Moiety and Their Antimicrobial Evaluation

We reported the environmental benign synthesis of chalcones,2-pyrazolines and cyclohexanones under microwave irradiation.Chalcones were obtained by the condensation of each of 2-hydroxyacetophenone derivatives with a-naphthaldehyde under microwave irradiation.The condensation reactions of each of synthesized chalcones with phenyl hydrazine under microwave irradiation in the presence of dry acetic acid as a cyclizing agent gave 2-pyrazolines.Also,the new cyclohexenone derivatives,valuable intermediates to synthesize fused heterocycles,have been prepared by the cyclocondensation of each of hydroxychalcones with ethyl acetoacetate.The structures of the synthesized compounds were elucidated by infrared（IR） spectrometry,nuclear magnetic resonance（NMR）,mass spectrometry（MS） and elmental analysis.The results indicate that unlike classical heating,microwave irradiation results in higher yields with shorter and cleaner reactions.The synthesized compounds were screened for antimicrobial activity against Staphylococcus aureus,Escherichia coli,Candida Albicans and Aspergillus niger.We clarified the effects of different substituents in the tested compounds on the obtaind antibacterial activities and antifungal activities.     

四种不同形状微粒的摩擦学性能研究

为了减少润滑液对石油的依赖,发展环境友好水基润滑液,本文通过在蒸馏水中加入螺旋藻、白葡萄球菌、寡核苷酸和丝素四种水溶性微粒来改善水的摩擦学特性.采用原子力显微镜和扫描电子显微镜对螺旋藻、白葡萄球菌、寡核苷酸和丝素进行结构表征;采用微摩擦试验仪对蒸馏水和四种水基润滑液摩擦性能进行了评价,采用流变仪对其流变性进行了评价.结果表明:1螺旋藻结构为螺旋状,白葡萄球菌为球状,寡核苷酸和丝素为线状;2螺旋藻的减摩效果最好,白葡萄球菌次之,最后是寡核苷酸和丝素;随着载荷增大蒸馏水摩擦系数减小,四种水基润滑液摩擦系数均增大;随着滑动速度增大摩擦系数均减小;3蒸馏水中添加微粒后仍为牛顿流体,但黏度增大.通过对机理进行分析,认为螺旋藻减摩效果最好是由于表面的螺旋结构能够产生足够大的滑动摩擦力,容易克服滚动阻力偶使微粒滚动起来;载荷增大四种水基润滑液摩擦系数均增大是微粒滚动阻力偶增大造成的;滑动速度增大四种水基润滑液摩擦系数均减小是微粒聚集体逐渐被分解造成的.     

Synthesis and Crystal Structure of 2,4-Diiodo-6-[(2-morpholin-4-yl-ethylimino)-methyl]-phenol-copper(Ⅱ)

2,4-Diiodo-6-[(2-morpholin-4-yl-ethylimino)-methyl]-phenol-copper(Ⅱ) has been designed and synthesized.The structure was determined by UV,IR and single-crystal X-ray study.The title complex crystallizes in the triclinic system,space group P1 with a=6.6090(13),b=10.377(2),c=12.550(3),α=113.29(3),β=93.84(3),γ=98.31(3)°,V=774.9(3)3,Dc=2.215g/cm3,C26H30CuO4N4I4,Mr=1033.68,F(000)=487,μ=4.726 mm-1,Z=1,the final R=0.0563 and wR=0.1475 for 2101 observed reflections(I2σ(I)).The central copper(Ⅱ) is four-coordinated by two nitrogen atoms and two oxygen atoms from two 3,5-diiodosalicylaldehyde-2-morpholinoethylamine Schiff bases.The complex is linked into a column by π-π stacking interaction.The complex was assayed for antibacterial activities against three Gram positive bacterial strains(B.subtilis,S.aureus and S.faecalis) and three Gram negative bacterial strains(E.coli,P.aeruginosa and E.cloacae) by MTT method.Fortunately,the title complex shows potent antibacterial activity against these six bacterial strains.