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偏振显微拉曼光谱(Polarized micro-Raman spectra, PMRS)技术可提供样品中分子的取向信息和有序性,并且无需染色或特殊的样本制备过程。本研究将该技术用于乳腺癌的研究,分别采集正常和癌变乳腺组织的拉曼光谱,光谱分析和差异性分析结果表明,PMRS技术可使酰胺Ⅰ带以外的主要特征带差异得以显著放大,如在常规显微拉曼光谱中没有明显差异的脯氨酸和苯丙氨酸特征带(921、 1032 cm-1)在PMRS中也有显著差异,证明PMRS技术具有更好的乳腺癌组织识别能力。研究了酰胺Ⅲ带I1247/I1269的光谱特性,结果表明,在癌变组织中,I1247/I1269在平行和垂直偏振光激发条件下都小于1;在正常组织中,I1247/I1269在平行和垂直偏振条件下则分别小于1和大于1,表明癌变后胶原纤维失去了原有的方向性,同时证明这与癌变后胶原纤维的羟基化即羟基化氨基酸含量增加有关。本研究结果有助于更好地阐明乳腺癌侵袭过程中诱导浸润... 相似文献
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Differential proteins expressing in ectopic and eutopic endometria were investigated by means of proteomic analysis. Five patients in secretary phase were diagnosed as endometriosis by laparoscopy. The five ectopic endometria(two at stage Ⅱ, two at stage Ⅲ and one at stage IV) and five eutopic endometria were surgically excised. One-dimensional electrophoresis coupled with liquid chromatography and mass spectrometry was used to screen and identify differential proteins. Three differential bands in one-dimensional electrophoresis were resolved by liquid chromatography and mass spectrometry and 14 up-regulated proteins were identified, including collagen α-1, α-2, α-3(VI), α-1(XIV) chain, actin, annexin A2, EMILIN-1, ferritin light polypeptide variant, fucosyltransferase 10, myosin-9, protein S100-A9, KIAA1783 protein, and two hypothetical proteins. Our data provides a list of potential biomarkers for endometriosis. The identifications may be used to develop new diagnoses for endometriosis. 相似文献
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Al2O3/SnO2 co-nanoparticles were prepared with a modified sol-gel technique followed by a thermal treatment process. With these co-nanoparticles the grafted collagen-Al2O3/SnO2 nanocomposites were obtained using a supersonic dispersion method. X-ray diffraction, FT-IR analysis, transmission electron microscopy, TGA/DTA and infrared emissivity test were performed to characterize the resulting nanoparticles and nanocomposites, respectively. The Al2O3/SnO2 co-nanoparticles showed a narrow distribution of size between 20-40 nm and could be uniformly absorbed on the tri-helix scaffolds of the grafted collagen without any aggregation. The nanocomposites possessed better thermal stability and substantially lower infrared emissivity than the grafted collagen and Al2O3/SnO2 co-nanoparticles with an increase of degradation temperature from 39 to 210 ℃ and a decrease of infrared emissivity from 0.850 of the grafted collagen and 0.708 of the Al2O3/SnO2 co-nanoparticles to 0.424, which provided a potential application of the nanocomposites to areas such as photoelectronics. 相似文献
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胶原蛋白是皮肤组织中的结构性蛋白,其数量和健康关系到外表皱纹的生成与皮肤的老化现象;而抗坏血酸有良好的美白与抗氧化能力,可使肌肤外表更白皙亮丽和保持健康。由于胶原蛋白具有自体荧光,因此我们提出一个藉由皮肤光谱来检验胶原蛋白含量的方法,此检测法更具实时性与非侵入性。本研究使用胶原蛋白和抗坏血酸,涂抹于健康的、与有斑点、皱纹等问题的肌肤,以其荧光与反射光谱随时间的变化,归纳出胶原蛋白与抗坏血酸对不同部位肌肤的吸收难易与修复疗效。结果显示,皮肤会对适当浓度的胶原蛋白或抗坏血酸之吸收效果较佳;加上适当浓度的抗坏血酸,胶原蛋白可被有效吸收同时产生良好的除皱效果;而抗坏血酸之去斑效果也在此研究中明确证实。藉由此一系列科学性的实验,可确实验证美容保养品的作用,帮助爱美人士对化妆保养品的正确选择与正确使用,发挥最好的疗效。 相似文献
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反相高效液相色谱法测定人肌腱中的胶原蛋白 总被引:3,自引:0,他引:3
建立了测定人肌腱中胶原蛋白含量的高效液相色谱法。动物肌腱中的胶原蛋白经酸水解后生成包括羟脯氨酸在内的氨基酸混合物,用2,4-二硝基氯苯对水解产物衍生化,然后以0.01 mol/L乙酸钠-乙酸缓冲液(pH 6.0)-乙腈(体积比为80∶20)为流动相,经反相C18柱分离,紫外检测器于360 nm波长处检测来测定羟脯氨酸的含量。羟脯氨酸是胶原蛋白的特异性氨基酸且含量稳定,因而可通过样品中羟脯氨酸的含量来计算胶原蛋白的含量。该方法对羟脯氨酸的检出限为3 μg/L,羟脯氨酸为3 μg/L~100 mg/L时与峰面积的线性关系良好;样品测定的相对标准偏差为1.95%,加标回收率为98.4%~110.8%。对60份人肌腱样品中胶原蛋白的含量进行了测定。所建立的方法灵敏、准确、干扰少,适用于肌腱中胶原蛋白含量的测定。 相似文献
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水解胶原蛋白热氧化及CaSO4形成机理探讨;水解胶原蛋白; L-抗坏血酸; H2O2; 热氧化; CaSO4 相似文献
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目的通过多中心随机对照的临床试验研究,比较以胶原蛋白为基质的新型肠内营养制剂与标准型肠内营养制剂对患者营养状况的影响,以了解新型肠内营养制剂的临床应用效果。方法本研究共6家医疗单位共同参与,共收集91例,分为对照组(标准肠内营养)及干预组(以胶原蛋白为基质的肠内营养),管饲补充104.6~125.5kJ(25~30kcal)/(kg·d),共7d,检测研究开始前后患者体格指标、营养相关指标、安全性指标等。结果组内比较显示两组患者在管饲两种不同的肠内营养制剂后营养风险筛查评分(NRS2002)较管饲前均有所降低(均P<0.025);组间差值比较显示患者营养状况、营养风险筛查(NRS2002)评分、胃肠道不良反应在两组间均无统计学差异(均P>0.025)。结论以胶原蛋白为基础经氨基酸模式优化后的肠内营养制剂与标准型肠内营养制剂均可以有效改善患者的营养状况,有助于促进患者的疾病预后。 相似文献