部分还原氧化石墨烯的制备、 表征及对人宫颈癌HeLa细胞的体外杀伤作用

以氧化石墨烯(GO)为原料, 利用温和方法制备了3种不同还原程度的部分还原氧化石墨烯pRGO₁, pRGO₂和pRGO₃(pRGO_1—3)； 利用傅里叶变换红外光谱(FTIR)、 拉曼光谱(Raman)、 X 射线光电子能谱(XPS)、 紫外-可见光谱(UV-Vis)、 透射电子显微镜(TEM)和 EDS能谱对其结构和形貌进行了表征. 细胞实验结果表明, 无激光照射下pRGO_1—3本身的细胞毒性较低; 近红外(NIR)激光照射下pRGO_1—3通过光热和光毒性双重作用杀伤肿瘤细胞. 实验结果显示了pRGO 在肿瘤光热疗法和光动力疗法领域的应用潜力.     

Exendin-4螺旋结构对生物活性及稳定性的影响

针对Exendin-4的螺旋结构特点, 采用去掉螺旋序列、 用3个连续Ala替换螺旋以及Gln13替换为Tyr13的方式对N端α-螺旋及C端α-螺旋进行改造, 设计出4个结构模拟物. 通过圆二光谱和荧光光谱进行结构分析, 结合模拟物生物活性实验, 分析Exendin-4螺旋结构与生物活性的关系. 结果表明：  Exendin-4的N端螺旋比C端螺旋对生物活性影响更大； 在抗二肽基肽酶（DPPⅣ酶）的稳定性实验中, C端螺旋比N端螺旋具有更好维系Exendin-4稳定性的作用； 螺旋结构中Tyr13可作为提高Exendin-4生物活性的重要优化位点.     

基于深度学习的中医古文献临床经验抽取

中医古文献蕴藏着丰富的临床经验，是古代中医在行医过程中对临床诊疗的经验性总结，体现了中医学形成和发展的理论框架和思想基础.然而这些宝贵的临床经验不仅量大，而且分散在不同的文献中，使得中医从业者手工很难快速全面地获取它们，文献检索工具也只能提供文档级别的信息筛选，无法为这种细粒度的信息获取提供支持.此外，古汉语相对于现代汉语的不同特点也限制了主流文本分析工具的使用效果.为此本文提出面向临床经验获取的中医古文献信息抽取任务，用于识别古文献中描述临床经验的文本片段，手工标注了样本数据用于这种抽取模型的训练和测试，并设计了基于深度学习的序列标注器用于完成该任务.考虑到标注数据量小可能带来的过度拟合问题，本文引入对抗训练和虚拟对抗训练来增强模型的泛化能力.一系列充分的实验验证了模型的有效性，表明利用信息抽取技术从古文献获取中医临床经验具有可行性，为这一新的信息抽取任务提供了有希望的研究基线和可复用的标注数据集.     

人才吸引力因素与政策工具契合关系研究以河北省为例

人才是第一资源,人才引进培养是经济社会发展的一个关键,近年来我国各地纷纷出台吸引人才的重磅政策.为探求人才政策工具对人才的吸引力情况,在构建人才吸引力因素理论模型基础上,通过对178位人才问卷调查获得人才吸引力关键因素,对河北省163份人才政策文本进行数据挖掘,分析政策工具与人才吸引力因素的契合关系.研究得出创新氛围、人才培训培养、人才激励评价、创新创业支持、人才服务和薪资待遇等方面契合度高,政策效果较佳;工作场所仪器设备条件、配偶子女安置和住房补贴的契合关系为政策不足,应加大政策制定;人才载体和医疗社会保障的契合关系为政策冗余,需加强政策实施.     

Ultrahigh Loading Copper Single Atom Catalyst for Palladium-free Wacker Oxidation

Wacker oxidation is an industry-adopted process to transform olefins into value-added epoxides and carbonyls. However, traditional Wacker oxidation involves the use of homogeneous palladium and copper catalysts for the olefin addition and reductive elimination. Here, we demonstrated an ultrahigh loading Cu single atom catalyst(14% Cu, mass fraction) for the palladium-free Wacker oxidation of 4-vinylanisole into the corresponding ketone with N-methylhydroxylamine hydrochloride as an additive under mild conditions. Mechanistic studies by ¹⁸O and deuterium isotope labelling revealed a hydrogen shift mechanism in this palladium-free process using N-methylhydroxylamine hydrochloride as the oxygen source. The reaction scope can be further extended to Kucherov oxidation. Our study paves the way to replace noble metal catalysts in the traditional homogeneous processes with single atom catalysts.     

基于MD-LBP关联方向特征的铁路隧道漏缆卡扣检测算法

全路段跟车拍摄隧道漏缆卡扣图像再进行逐张排查,是实现卡扣故障检测的重要手段.针对目前LBP、CS-LBP等相关变体算法存在描述子质量不佳、特征维度过高的问题,提出MD-LBP关联方向特征提取算法实现故障卡扣的检测工作.该算法首先对输入图像进行高斯滤波预处理,根据图像的全局灰度均值得到图像的自适应阈值;其次计算图像的三层MD-LBP特征图结构,依次经过两次下采样分别得到Cell主方向特征和Block主方向特征;然后再在Block特征图上提取关联方向特征,并以此作为描述子;最后通过SVM区分故障卡扣,完成检测工作.实验还对比了LBP、H OG等9种特征描述子的检测情况,结果表明该算法不仅特征维度低,并且检测故障卡扣的召回率和精准度都达到了85％.     

Understanding the solubility and crystallization of molybdenum in high-sodium borosilicate glasses: effect of lanthanum oxide

Journal of Radioanalytical and Nuclear Chemistry - The simplified 8-oxide system (SiO2-B2O3-Na2O-Al2O3-CaO-TiO2-MoO3-La2O3) has been prepared under two types of cooling methods, and analyzed with...     

Hitherto-Unexplored Photodynamic Therapy of Ag2S and Enhanced Regulation Based on Polydopamine In Vitro and Vivo

Given their superior penetration depths, photosensitizers with longer absorption wavelengths present broader application prospects in photodynamic therapy (PDT). Herein, Ag₂S quantum dots were discovered, for the first time, to be capable of killing tumor cells through the photodynamic route by near-infrared light irradiation, which means relatively less excitation of the probe compared with traditional photosensitizers absorbing short wavelengths. On modification with polydopamine (PDA), PDA-Ag₂S was obtained, which showed outstanding capacity for inducing reactive oxygen species (increased by 1.69 times). With the addition of PDA, Ag₂S had more opportunities to react with surrounding O₂, which was demonstrated by typical triplet electron spin resonance (ESR) analysis. Furthermore, the PDT effects of Ag₂S and PDA-Ag₂S achieved at longer wavelengths were almost identical to the effects produced at 660 nm, which was proved by studies in vitro. PDA-Ag₂S showed distinctly better therapeutic effects than Ag₂S in experiments in vivo, which further validated the enhanced regulatory effect of PDA. Altogether, a new photosensitizer with longer absorption wavelength was developed by using the hitherto-unexplored photodynamic function of Ag₂S quantum dots, which extended and enhanced the regulatory effect originating from PDA.