HL-60多药抗性细胞抗HT诱导凋亡机制的研究

用1mg·L-1三尖杉酯碱(Harringtoning,HT)处理人早幼粒急性白血病HL-60细胞2h,诱导细胞出现明显的凋亡(Apoptosis,Apo)形态学和生化特征,包括染色质凝集、Caspase-3(天冬氨酸特异的半胱氨酸蛋白水解酶-3)活化、DNA断裂等.来自HL-60的多药抗性细胞HR20和HT9抗HT诱导的凋亡,一般认为是P-糖蛋白(P-GP-170)对药物的外排作用引起细胞对凋亡的抗性.但用2mg·L-1 CsA(Cysclosporine A)逆转抗性细胞对药物的外排作用,1mg·L-1 HT作用48h仍不能诱导HR20和HT9细胞凋亡,说明HR20和HT9多药抗性细胞抗HT诱导凋亡的机制不仅仅在于细胞膜上的P-糖蛋白对药物的外排作用,可能还有凋亡相关因子或其他因素参与作用.Caspase-3是细胞凋亡通路中的一个中心环节,HR20和HT9抗HT诱导的Caspase-3活化,这可能是HR20和HT9不能凋亡的一个重要原因.     

抗癌药物阿霉素的荧光发射光谱分析

利用蒽环类抗癌药物阿霉素的自发荧光可反映药物在细胞中的分布和相对剂量,利用特异性荧光探针Ｈｏｅｃｈｓｔ３３３４２（Ｈｏ．３３３４２）可标记活细胞ＤＮＡ,从而反映细胞所处周期时相及染色体的形态变化,但是在阿霉素和Ｈｏ．３３３４２共存在的细胞中,用ＭＰＶＩＩ型显微荧光分光光度计对药的的自发荧光和Ｈｏ．３３３４２的发射芝光进行光谱分析的结果表明：在紫外激发下,药物自我荧光与Ｈｏ．３３３４２荧光之间存在干     

Evolutionary Prisoners' Dilemma Game on Scale-Free Networks with Degree-Degree Correlation

Degree-degree correlation and heterogeneity in degree are important topological properties characterizing scale-free networks. We consider an evolutionary prisoners＇ dilemma game on scale-free networks and investigate how degree-degree correlation influences cooperation. It is found that the cooperator frequency displays resonance-like behavior with the variation of Pearson correlation coefficient. A measure on local heterogeneity in a network is proposed and it is realized that cooperation is proportional to the local heterogeneity.     

Evolutionary Prisoner＇s Dilemma on a Weighted Scale-Free Network

We investigate the evolutionary prisoner＇s dilemma on a weighted scale-free network where the diversity of teaching ability is introduced to the network in the form of weight. Though the diversity of teaching ability is not a sufficient condition for the enhancement of cooperation, we find that the degree-dependent teaching ability plays an active role in the evolution of cooperation. A new phenomenon is found when the degree-dependent teaching ability is used： the distribution of the cooperator frequency displays a two-peak structure for a certain parameter range. We also investigate the effects of the degree-degree correlation of the network on the evolution of cooperation in the presence of the diversity of the teaching ability.     

Effects of Degree on Cooperation in Evolutionary Prisoner's Dilemma Games on Structured Populations

We focus on the effects of individuals＇ degrees on spontaneous cooperation. By investigating evolutionary PDGs on random networks with two distinct degrees, we find some resonance-type behaviors of the cooperator frequency with the variation of degree. We also find that increasing hums degree actually disfavors the cooperation though the heterogeneity in degree can enhance cooperation in the population.     

C60衍生物修饰的固体支撑双分子层磷脂膜(s-BLM)分子器件的电化学和拉曼光谱研究

本文配合拉曼光谱技术比较了以不同的C60衍生物修饰的固体支撑双分子层磷脂膜（s-BLM)分子器件体系对I^-的检测灵敏度，结果表明，一些衍生物可使s-BLM体系对I^-的检测灵敏度进一步提高，显示了C60衍生物在BLM分子器件中良好的应用前景。     

Bcl-2和Caspase-3在HT诱导的HL-60细胞凋亡中的作用

利用常规的HL－60细胞和转染了 bcl－2基因的HL－60/Bcl-2细胞及转染了空载体的对照HL－60／neo细胞为模型,研究Caspase-3及过表达的Bcl-2对凋亡过程中的各种生化,形态学特征的影响,探讨了Caspase-3及Bcl-2在三尖杉酯碱（HT）诱导的HL－60细胞凋亡中的作用,研究表明,Caspase-3的湃化在HT诱导的HL－60细胞凋亡中处于关键地位,导致了质膜出泡,PS翻转,染色质凝集,DNA断裂及凋亡下游的发生,而Bcl-2则通过抑制aspase-3的活化抑制了凋亡的发生,同时还证明,在HT诱导的HL－60细胞凋亡中,通常被认为是凋亡早期特征的磷脂酰丝氨酸PS翻转并不是一个早期特征,它至少晚于Caspase-3的活化,甚至不早于质膜出泡及染色质凝集。