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1.
Low-flow chromatography has a rich history of innovation but has yet to reach widespread implementation in bioanalytical applications. Improvements in pump technology, microfluidic connections, and nano-electrospray sources for MS have laid the groundwork for broader application, and innovation in this space has accelerated in recent years. This article reviews the instrumentation used for nano-flow LC, the types of columns employed, and strategies for multidimensionality of separations, which are key to the future state of the technique to the high-throughput needs of modern bioanalysis. An update of the current applications where nano-LC is widely used, such as proteomics and metabolomics, is discussed. But the trend toward biopharmaceutical development of increasingly complex, targeted, and potent therapeutics for the safe treatment of disease drives the need for ultimate selectivity and sensitivity of our analytical platforms for targeted quantitation in a regulated space. The selectivity needs are best addressed by mass spectrometric detection, especially at high resolutions, and exquisite sensitivity is provided by nano-electrospray ionization as the technology continues to evolve into an accessible, robust, and easy-to-use platform. 相似文献
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对网格环境下基于本体的专家系统(Expert System)进行了研究,提出了构建在通用知识网格(Universal Knowledge Grid,UKG)和开放网格业务体系结构(Open Grid Services Architecture,OGSA)基础上的专家系统模型。为网格环境下创建大规模分布式基于本体知识库体系的专家系统提供了参考模型。 相似文献
4.
B.‐J. Fang Y.‐J. Shan H.‐Q. Xu H.‐S. Luo Z.‐W. Yin 《Advanced functional materials》2004,14(2):169-173
Pb[(Zn1/3Nb2/3)0.91Ti0.09]O3 (PZNT91/9) single crystals were grown by two methods: from solution using PbO as a self‐fluxing agent (SC method) and directly from the melt without fluxing (MC method). In both growth methods, an allomeric Pb[(Mg1/3‐Nb2/3)0.69Ti0.31]O3 (PMNT69/31) single crystal was used as a seed. X‐ray diffraction patterns of ground crystals showed that phase‐pure perovskite PZNT91/9 single crystals were successfully fabricated by the above two methods. The composition of the crystals obtained by both the SC and MC methods was analyzed using X‐ray fluorescence, which confirmed that the crystal composition is close to the nominal value, although volatilization of PbO and segregation during crystal growth are inevitable. The MC PZNT91/9 crystals exhibit excellent piezoelectric properties, with the piezoelectric constant, d33, in the range of 1800–2200 pC N–1. This value is comparable to that of the SC crystals. However, the MC crystals show an abnormal dielectric behavior. In contrast with the SC crystals, in the MC crystals a much broader dielectric peak appears in the dielectric response curves, accompanied by a much lower peak temperature of around 105 °C. Furthermore, frequency dispersion is apparent over a much wider temperature range (even more apparent than in pure relaxors), where a large, i.e., about 70 °C, full width at half maximum (FWHM) for the dielectric peaks is observed in the dielectric response. It is speculated that such an unusual phenomenon correlates with defects, microinhomogeneities, and polar regions in the as‐grown MC crystals. The origins of this abnormality have not been interpreted in detail until now. However, optical observation of the domain structure confirms that both the SC and MC crystals possess complex structural states. 相似文献
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文章主要讨论了LonWorks现场总线技术在城市污水处理厂和气象指数预报中的应用。采用IPC和PLC控制结构对现场的各种设备实现控制和数据的采集,通过现场总线网络传输数据到中心控制室或远程终端,实现污水处理的自动控制和气象信息的实时采集和处理。 相似文献
6.
The breakthrough and stoichiometric SO2 adsorption efficiencies of a biomass supported Na2CO3 system (80 wt %Na2CO3/straw) have reached 48.9% and 80.6% respectively at a desulfurization temperature of 80℃. 相似文献
7.
利用国家同步辐射实验室合肥光源的真空紫外同步辐射,使NO分子和Ar原子混合物的超声分子束发生光电离,测量了Ar,NO和异类团簇Ar·NO的光电离效率谱. 在谱中,在与Ar原子的共振线对应的能量区域(11.5—12.0 eV)观察到一个强的类共振结构. 这个结果表明,在异类团簇Ar·NO的内部,稀有气体Ar原子的激发能转移到与它接触的分子NO上,使分子NO发生电离.
关键词:
Ar·NO团簇
同步辐射
光电离
能量转移 相似文献
8.
移动增值业务在全球的发展速度依然很快,非话音收入占电信总收入的比重越来越大.在移动增值业务的发展上,日本遥遥领先,韩国紧随其后.在中国,虽然短信业务收入开始下降,但无线应用协议(WAP)、交互式语音回应(lVR)等业务收入快速攀升,从而保证了移动增值业务在中国的发展速度.由于中国信息产业部整顿收费混乱的移动增值业务市场,使服务提供商/内容提供商(SP/CP)的暴利时代成为过去,增值业务市场进入精耕细作期.随着用户对移动增值业务新的需求的不断增长,整首音乐下载、移动E-mail以及手机电视等业务将成为未来全球最有前途的移动增值业务. 相似文献
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MRI fuzzy segmentation of brain tissue using neighborhood attraction with neural-network optimization. 总被引:11,自引:0,他引:11
Shan Shen William Sandham Malcolm Granat Annette Sterr 《IEEE transactions on information technology in biomedicine》2005,9(3):459-467
Image segmentation is an indispensable process in the visualization of human tissues, particularly during clinical analysis of magnetic resonance (MR) images. Unfortunately, MR images always contain a significant amount of noise caused by operator performance, equipment, and the environment, which can lead to serious inaccuracies with segmentation. A robust segmentation technique based on an extension to the traditional fuzzy c-means (FCM) clustering algorithm is proposed in this paper. A neighborhood attraction, which is dependent on the relative location and features of neighboring pixels, is shown to improve the segmentation performance dramatically. The degree of attraction is optimized by a neural-network model. Simulated and real brain MR images with different noise levels are segmented to demonstrate the superiority of the proposed technique compared to other FCM-based methods. This segmentation method is a key component of an MR image-based classification system for brain tumors, currently being developed. Index Terms-Improved fuzzy c-means clustering (IFCM), magnetic resonance imaging (MRI), neighborhood attraction, segmentation. 相似文献