基于生物质谱技术的磷酰化修饰策略在多肽测序中的应用

该文建立了一种利用磷酰化修饰结合电喷雾质谱(ESI-Q-TOF)测定多肽氨基酸序列的有效方法。利用Atherton-Todd反应,以二丙基亚磷酰酯(DPP)为磷酰化试剂,应用生物质谱技术,对磷酰化修饰后的5种模型肽的磷酰化反应情况进行了系统研究,考察了磷酰化肽的二级质谱特征,并与未经磷酰化反应的肽的二级质谱特征对比。结果表明,经过磷酰化修饰后,肽的二级质谱中的a1离子信号强度明显增加,可以准确鉴定其N端氨基酸;b系列离子信息完整,信号强度增强,使得多肽C ID测序的谱图简单、清晰,有利于肽的氨基酸序列的测定;赖氨酸(K,128.10 u)和谷氨酰胺(Q,128.13 u)两种氨基酸质荷比相近,由于二者磷酰化修饰后的差异性,使其得到准确区分。经过5种已知氨基酸序列的模型肽的磷酰化后结合质谱技术进行氨基酸序列测定验证,结果表明该方法简单、快速、准确,提高了利用质谱技术进行多肽测序的准确度和灵敏度,可为蛋白质组学研究提供有效的技术手段。     

A position-sensitive detector with lithium glass and MaPMT

A position-sensitive detector is designed for neutron detection. It uses a single continuous screen of a self-made lithium glass scintillator, rather than discrete crystal implementations, coupling with a multi-anode PMT (MaPMT). The scintillator is fast and efficient; with a decay time of 34 ns and thermal neutron detection efficiency of around 95.8% for the 3 mm thick screen, and its light yield is around 5670 photons per neutron and 3768 photons per MeV γ rays deposition. The spatial resolution is around 1.6 mm (FWHM) with the energy resolution around 34.7% by using α (5.2 MeV) rays test.     

UPS离线维护好伙伴－－SBP

UPS通过电池不间断地提供着电源供应,对于所有的机房来说,UPS的在线使用时间是最长的,365×24不间断运行!哪怕是服务器停机维护,UPS也在为网络设备提供源源不断的电力支持。 也许,我们“有幸”看过爆浆的UPS的电池,如果再幸运一点,有可能遇到UPS的应用故障,这个时候我们该如何处理呢？     

蚁群算法在110警车巡逻方案中的应用

蚁群算法是一种新型智能仿生类算法。以近年来国内外学者提出的蚁群算法思想为基础,并结合了城市道路的实际情况,为了更好的解决110警车巡逻路线问题,建立了智能局部自适应最大区域覆盖模型,同时应用了最短路径优化算法,通过实例来验证算法改进的合理实用性和模型假设的科学有效性,为大规模的预警巡逻提供了理论和应用支持。从而为警车巡逻在控制社会治安问题中发挥着越来越重要的作用。     

基于TMS320DM355的VGA信号实时监控系统

提出了以TMS320DM355为核心,对VGA信号进行实时采集、编码和传输的系统方案。描述了基于TMS320DM355和TVP7002的系统总体硬件框架,分析了视频采集、编码等模块的软件实现过程,重点介绍对采集设备进行初始化时的硬件寄存器配置,最后利用开源的RTSP流媒体服务器wis-streamer实现MPEG-4码流的传输。     

Cosmic ray test for a T0 detector

The endcap of time-of-flight (ETOF) detector in BES (Beijing Spectrometer) III is planned to be upgraded by using multi-gap resistive plate chambers (MRPCs) and the designed time resolution of the MRPCs is around 50 ps. Thus a time-zero (T0) detector needs to be built to offer a high quality reference time for the MRPCs beam test. So a T0 detector is built using plastic scintillator tiles (BC420) to couple with four fast phototubes (PMTs, Hamamatsu H6533). The timing properties of the detector is studied by using a cosmic ray test and factors related to the time resolution, such as plastic scintillator size, readout mode and angle effects, are discussed. T0 detector timing resolutions of ～41-62 ps are achieved, which means that the T0 detector can be used in the MRPC beam test.     

浅析智能管道及其解决方案

在通信产业链中,以语音业务为主要收入来源的电信运营商曾经是活得最滋润的。但近年来,随着互联网业务飞速发展,新兴的数据业务在消费者的信息通信行为中所占比例越来越大,单用户的ARPU值却停步不前;同时,以谷歌、腾讯、苹果为代表的服务和终端提供商却在几乎没有对网络带宽进行投资的情况下,利用运营商网络为用户提供了丰富多彩的业务。并从中赚取了大量的利润。     

Experimental validation of domain-level gradient-based and hierarchical PCE-based routing and control in heterogeneous optical networks

A domain-level gradient-based routing （DLR） algorithm for heterogeneous optical networks with syn- chronous digital hierarchy and optical transport network domains is proposed and experimentally vali- dated. This algorithm classifies domains into groups with incremental levels on the basis of domain-level partitioning, and guides paths level by level along a gradient on the basis of interdomain routing tree evolution. The proposed algorithm is implemented in the hierarchical path computation element-based control architecture for connection provisioning. Testbeds with commercial and emulated nodes are es- tablished to verify the feasibility and performance of the algorithm. Experimental and emulation results show that DLR effectively performs in terms of network blocking probability, real time characteristics, and scalability.     

鼻内镜下YAG激光治疗咽囊囊肿

目的:目探讨在鼻内镜下YAC激光治疗咽囊囊肿的方法及疗效。方法:在鼻内镜下利用YAG激光治疗咽囊囊肿13例,1例伴有鼻息肉,鼻内镜下利用电动切削器切除,余12例在鼻内镜下利用YAG激光治疗,其中咽囊脓肿3例。结果:经1至2年随访,总有效率达100%,无并发症。结论:在鼻内镜下利用YAG激光治疗咽囊囊肿,该方法视野清晰,操作简单,出血少,病人痛苦小,门诊即可完成,疗效可靠,值得推广。