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Optical sectioning in wide-field microscopy is achieved by illumination of the object with a continuously moving single-spatial-frequency pattern and detecting the image with a smart pixel detector array. This detector performs an on-chip electronic signal processing that extracts the optically sectioned image. The optically sectioned image is directly observed in real time without any additional postprocessing. 相似文献
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Tegy J. Vadakkan James C. Culver Liang Gao Tiemo Anhut Mary E. Dickinson 《Journal of fluorescence》2009,19(6):1103-1109
mCherry is a red fluorescent protein which is bright, photostable, and has a low molecular weight. It is an attractive choice
for multiphoton fluorescence imaging; however, the multiphoton excitation spectrum of mCherry is not known. In this paper
we report the two photon excitation spectrum of mCherry measured up to 1190 nm in the near infrared (NIR) region. Skin tissues
of transgenic mice that express mCherry were used in the experiments. mCherry in the tissues was excited with a Titanium:Sapphire
laser and an optical parametric oscillator pumped by the Titanium:Sapphire laser. We found that the peak excitation of mCherry
occurs at 1160 nm. 相似文献
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