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排序方式: 共有141条查询结果,搜索用时 31 毫秒
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The effect of the bifunctional crosslinking reagent glutaraldehyde on the activity of the restriction enzymes Bam HI,Hind III, EcoRI, and Tthlll I was investigated. The four enzymes exhibited differential sensitivity to inactivation. Tthlll I
was the most sensitive, with activity losses occurring at levels of 0.0025% and above.Hind III was the most stable of the four and remained fully active at concentrations as high as 0.075%. Addition of BSA to incubation
mixtures generally had a stabilizing effect. Implications of these results for the design of glutaraldehyde-based methods
for the immobilization of restriction endonucleases are discussed. 相似文献
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Bogdan Boduszek Tomasz Olszewski Waldemar Goldeman Magdalena Konieczna 《Phosphorus, sulfur, and silicon and the related elements》2013,188(4):787-795
The synthesis of a series of new pyridine aminomethylphosphinic acids is described. These compounds were obtained in the reaction of the corresponding pyridine aldehydes with primary amines and with ethyl phenylphosphinate, or methylphosphinate, in the presence of bromotrimethylsilane. In aqueous, strong acid solutions, pyridine aminophosphinic acids were split, forming the phenyl-, or methylphosphonic, acid and the corresponding secondary pyridyl-alkylamines. The kinetics of some observed cleavages were measured, and a mechanism of the cleavage has been proposed. 相似文献
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M.?Paczwa A.?A.?Sapiga M.?Olszewski N.?A.?SergeevEmail author A.?V.?Sapiga 《Applied magnetic resonance》2015,46(5):583-592
The temperature dependences of nuclear magnetic resonance and magic angle spinning nuclear magnetic resonance spectra of 27Al nuclei in natrolite (Na2Al2Si3O10· 2H2O) have been studied. The influence of water molecules and sodium ions mobility on the shape of the 27Al NMR spectrum and framework dynamics have been discussed The temperature dependences of the spin–lattice relaxation times T1 of 27Al nuclei in natrolite have also been studied. It has been shown that the spin–lattice relaxation of the 27Al is governed by the electric quadrupole interaction with the crystal electric field gradients modulated by translational motion of H2O molecules in the natrolite pores. The dipolar interactions with paramagnetic impurities become significant as a relaxation mechanism of the 27Al nuclei only at low temperatures (<270 K). 相似文献
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Bilski P Olszewski M Sergeev NA Wasicki J 《Solid state nuclear magnetic resonance》2004,25(1-3):15-20
A general equation for the dipolar correlation function, to be used to analyze various kinds of independent internal motions, described by some correlation times tau(cm) (m = 1,2 em...k), has been obtained. The obtained expression has been used to analyze the temperature dependencies of different NMR measured values: second moment: spin-lattice relaxation times; amplitude of solid echoes signals. 相似文献
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Back BB Baker MD Ballintijn M Barton DS Becker B Betts RR Bickley AA Bindel R Budzanowski A Busza W Carroll A Decowski MP García E Gburek T George N Gulbrandsen K Gushue S Halliwell C Hamblen J Harrington AS Henderson C Hofman DJ Hollis RS Hołyński R Holzman B Iordanova A Johnson E Kane JL Khan N Kulinich P Kuo CM Lee JW Lin WT Manly S Mignerey AC Noell A Nouicer R Olszewski A Pak R Park IC Pernegger H Reed C Remsberg LP Roland C Roland G Sagerer J Sarin P Sawicki P Sedykh I Skulski W Smith CE 《Physical review letters》2003,91(7):072302
We have measured transverse momentum distributions of charged hadrons produced in d+Au collisions at sqrt[s(NN)]=200 GeV. The spectra were obtained for transverse momenta 0.25
相似文献