Investigations into the parallel kinetic resolution of 2-phenylpropanoyl chloride using quasi-enantiomeric oxazolidinones

The resolution of 2-phenylpropanoyl chloride using an equimolar combination of quasi-enantiomeric oxazolidinones is discussed. The levels of diastereoselectivity were found to be dependent upon the structural nature of the metallated oxazolidinone, temperature and metal counter-ion.     

Power spectrum analysis of the average-fluctuation density separation in interacting particle systems

The power spectrum analysis using the Lomb-Scargle false alarm probability statistic shows a clear separation between the average and fluctuating parts of the state density in embedded two-body random matrix ensembles with a mean-field for both fermion and boson systems as well as in the nuclear shell model.     

Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain

Background

Antiproliferative factor (APF) is a sialoglycopeptide elevated in the urine of patients with interstitial cystitis—a chronic, painful bladder disease. APF inhibits the proliferation of normal bladder epithelial cells and cancer cells in vitro, presumably by binding to its cellular receptor, cytoskeleton associated-protein 4 (CKAP4); however, the biophysical interaction of APF with CKAP4 has not been characterized previously. In this study, we used surface plasmon resonance (SPR) to explore the binding kinetics of the interaction of APF and as-APF (a desialylated APF analogue with full activity) to CKAP4.

Results

We immobilized non-glycosylated APF (TVPAAVVVA) to the Fc1 channel as the control and as-APF to Fc2 channel as the ligand in order to measure the binding of CKAP4 recombinant proteins encompassing only the extracellular domain (Aa 127–602) or the extracellular domain plus the transmembrane domain (Aa 106–602). Positive binding was detected to both CKAP4_126–602 and CKAP4_106–602, suggesting that as-APF can bind specifically to CKAP4 and that the potential binding site(s) are located within the extracellular domain. To identify the primary APF binding site(s) within the CKAP4 extracellular domain, deletion mutants were designed according to structural predictions, and the purified recombinant proteins were immobilized on a CM5 chip through amine-coupling to measure as-APF binding activity. Importantly, both CKAP4_127–360 and CKAP4_361–524 exhibited a fast association rate (k _on ) and a slow dissociation rate (k _off ), thus generating high binding affinity and suggesting that both regions contribute relatively equally to overall as-APF binding. Therefore, two or more as-APF binding sites may exist within the Aa 127–524 region of the CKAP4 extracellular domain.

Conclusions

We determined that the CKAP4_127–360 and CKAP4_361–524 mutants exhibit improved binding activity to as-APF as compared to the full-length extracellular domain, making it possible to detect low concentrations of as-APF in urine, thereby establishing a foundation for a non-invasive diagnostic assay for IC. Further, these data have revealed novel APF binding site(s) suggesting that targeting this region of CKAP4 to inhibit APF binding may be a useful strategy for treating IC-related bladder pathology.

     

含油岩心显微荧光成像光谱研究

发展了一种显微荧光光谱成像技术,并将其应用于天然岩心进行显微荧光成像光谱研究。利用这种技术同时采集含油岩心表面的荧光光谱信息和空间信息．并对获得的光谱图像给予光谱学和地质学解释。结果表明,不但能显示岩心形貌和组分的大致趋势,而且能揭示其精细细节．为石油地质研究提供了一种新方法,为今后的石油勘探开发工作提供了一种先进的指导手段。     

Dopant induced morphologies of ZnS nanoparticles

We have reported the synthesis of cubic (zinc blende) phase ZnS: Ni²⁺ nanoparticles using a simple wet‐chemical method. Synthesized ZnS: Ni²⁺ nanoparticles had been characterized by X‐ray diffraction (XRD) and Energy Dispersive X‐ray (EDX) analysis. Surface morphologies were studied using Scanning Electron Microscopy (SEM). Fourier Transform Infrared (FTIR) spectra of selected samples were also carried out to confirm the presence of capping agent on the surface of the material. We have demonstrated that various morphologies like spherical, tetrapods, sheet and long‐armed multipods are emerged by simple chemical route without any vigorous reaction parameters and changing the concentration of dopant ions only. The probable mechanism for such morphologies has also been suggested.     

Mössbauer Study of Magnetic FeSb Dilute System

Hyperfine Interactions - Mössbauer study of semimetals is of great importance in understanding the origin of hyperfine interactions in these materials. A concentration dependence study in Fe x...     

Model for inclusive production of protons,anti-protons pions and kaons with large transverse momenta

The production of particle with large transverse momenta P_T and other unexpected features observed with CERN ISR are explained on the basis of a model which traces their origin to a small class of events, called central collisions, involving “core-core” interactions. In each central collision, two massive fire balls, moving slowly in the C.M. system, are produced and they decay according to Fermi's statistical theory. The model makes specific predictions like a correlations between large P_T, high multiplicities and production of antiprotons.     

Analysis of a shielded TE011 mode composite dielectric resonator for stable frequency reference

Analysis of a TE₀₁₁ mode composite sapphire-rutile dielectric resonator has been carried out to study the temperature variation of resonance frequency, close to the Cs atomic clock hyperfine frequency of 9.192 GHz. The complementary behavior of dielectric permittivity with temperature of the composite has been exploited to obtain the desired turning point in the resonant frequency. The frequency of the composite structure is found to be independent of the shield diameter beyond four times the puck diameter.     

Production ofψ particles in N-N collisions and largeP T phenomenon

We calculate the yield ofψ (3105) particles in N-N collisions in a model which associates the production of heavy particles with largeP _T phenomenon. Our results show thatψ (3105) has a fairly strong coupling to other hadrons. We propose a criterion in the search for charmed particles and a parametrization for the expected yields of such particles.