DETECTION OF DNA-PSORALEN PHOTOADDUCTS in situ

Abstract— An immunological method, with the use of specific immune serum, has been developed for detection of 8-methoxypsoralen (8-MOP) photoadducts to DNA, formed in situ in cell nuclei, after combined treatment with 8MOP and UV-A irradiation (Zarçbska et al. , 1978). Lymphocytes fixed on slides or in suspension, and cryostat sections of different mammalian tissues, served as antigenic substrate, after treatment with 8-MOP and UV-A in vitro. Specific fluorescence in these substrates was detected in the nuclei after treatment with 30 ˜ 140 kJ/m² UV-A in the presence of 0.1-0.3 μg/cm² 8-MOP. PHA-stimulated-lymphocytes appeared to be the most sensitive substrate.
However, hairless mice treated with high doses of UV-A in vivo , 70 ˜ 360 kJ/m² did not reveal a specific fluorescence of epidermal nuclei, unless a high local concentration of 8-MOP was attained.
The apparent discrepancy in the level of photoadduct detection between the in vitro and in vivo treated specimens was explained by the low number of DNA-8-MOP-photoadducts formed in vivo under these experimental conditions. The relevance of these findings to the role of DNA-8-MOP-photoadducts formed during PUVA photochemotherapy is discussed.     

Time resolved resonance fluorescence study of electronically excited iodine atoms, I(5P1/2)

Electronically excited iodine atoms, I(5²P_1/2), have been monitored using time resolved atomic resonance fluorescence. Rate data for quenching by C₃H₈ and CD₄ are presented.     

ON QUOTIENTS OF BRAID GROUPS

ABSTRACT.

Let G be the group ?[t, t ^?1] x ?. By studying the action of the braid group B_n on the set Gⁿ , we obtain representations of B_n into a wreath product of the symmetric group and the general linear group over ?[t, t ^?1]. This in particular recovers the Burau representation of the braid group. Furthermore, some quotients of the braid group are obtained by using the representations found.     

Reconfigurable silicon filter with continuous bandwidth tunability

We present the design and the fabrication of compact tunable silicon-on-insulator bandpass filters based on the integration of a Mach-Zehnder interferometer with ring resonators and activated via thermo-optic phase shifters. The proposed architecture provides wide filter bandwidth tunability from 10% to 90% of the free spectral range preserving the filter off-band rejection. Possible applications are channel subset selection in wavelength division multiplexing optical systems, adaptive filtering to signal bandwidth, and reconfigurable filters for gridless networking.     

Tracer kinetics of plants carbon allocation with continuously produced11CO2

Parameters of carbon allocation dynamics in plants were measured using¹¹CO₂ and tracer kinetics techniques. Mechanical agitation reduced carbon export rate by 33% in cotton seedling's leaf, while storage rate and export pool size increased. Carbon storage and export rates of C₄ bunch grasses were higher in the afternoon than in the morning inspite of the decrease in CER.Water stress of cotton seedlings caused reduction in carbon export rate, and increase in exportable products concentrations and rate of storage. By the third stress day, measurable decreases in CER, transpiration, export rate and export pool size were recorded.     

Chromatographische Analyse

Ohne Zusammenfassung     

Re-evaluation of the mechanical loss factor of hydroxide-catalysis bonds and its significance for the next generation of gravitational wave detectors

New measurements of the mechanical loss factors of hydroxide-catalysis bonds have been made and these have been found to be significantly lower than previously reported. We provide the first estimate for the resulting thermal noise limitation for a practical test mass geometry for next generation gravitational wave detectors.     

Chromatography of leaf chloroplast pigments on ion-exchange papers

For the separation of leaf carotenoid pigments, some ion-exchange papers are more effective than pure cellulose papers. Separations of the major pigments on ion-exchange paper are superior to those obtained by uni-dimensional paper chromatography. For green-leaf extracts, ion-exchange papers offer no advantage. Ion-exchange columns do not separate the chloroplast pigments. On ion-exchange paper, there is no major yellow pigment in addition to neoxanthin, violaxanthin, lutein, and carotene.     

Characterization of partially transesterified poly(beta-hydroxyalkanoate)s using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for the characterization of a partially transesterified poly(beta-hydroxyalkanoate), PHA, polymer produced by the bacterial strain Alcaligenes eutrophus using saponified vegetable oils as the sole carbon sources. The transesterification was carried out separately under acidic and basic conditions to obtain PHA oligomers weighing less than 10 kDa. The intact oligomers were detected in their cationized [M + Na](+) and [M + K](+) forms by MALDI-TOFMS. A composition analysis, using the MALDI-TOF spectra, indicate that the oligomers obtained via acid catalysis were terminated with a methyl 3-hydroxybutyrate end group, and those obtained by base catalysis had a methyl crotonate (olefinic) termination. In addition to HB (hydroxy butyrate), the oligomers were found to contain a small percentage of HV (hydroxy valerate). This was independently confirmed using gas chromatography/mass spectrometry (GC/MS). In comparison, the analysis of a commercial PHA polymer, transesterified under identical conditions, only showed the presence of HB, i.e. a pure PHB homopolymer. Copyright 1999 John Wiley & Sons, Ltd.