Carotenoid photoprotection in artificial photosynthetic antennas

A series of phthalocyanine-carotenoid dyads in which a phenylamino group links a phthalocyanine to carotenoids having 8-11 backbone double bonds were examined by visible and near-infrared femtosecond pump-probe spectroscopy combined with global fitting analysis. The series of molecules has permitted investigation of the role of carotenoids in the quenching of excited states of cyclic tetrapyrroles. The transient behavior varied dramatically with the length of the carotenoid and the solvent environment. Clear spectroscopic signatures of radical species revealed photoinduced electron transfer as the main quenching mechanism for all dyads dissolved in a polar solvent (THF), and the quenching rate was almost independent of carotenoid length. However, in a nonpolar solvent (toluene), quenching rates displayed a strong dependence on the conjugation length of the carotenoid and the mechanism did not include charge separation. The lack of any rise time components of a carotenoid S(1) signature in all experiments in toluene suggests that an excitonic coupling between the carotenoid S(1) state and phthalocyanine Q state, rather than a conventional energy transfer process, is the major mechanism of quenching. A pronounced inhomogeneity of the system was observed and attributed to the presence of a phenyl-amino linker between phthalocyanine and carotenoids. On the basis of accumulated work on various caroteno-phthalocyanine dyads and triads, we have now identified three mechanisms of tetrapyrrole singlet excited state quenching by carotenoids in artificial systems: (i) Car-Pc electron transfer and recombination; (ii)(1) Pc to Car S(1) energy transfer and fast internal conversion to the Car ground state; (iii) excitonic coupling between (1)Pc and Car S(1) and ensuing internal conversion to the ground state of the carotenoid. The dominant mechanism depends upon the exact molecular architecture and solvent environment. These synthetic systems are providing a deeper understanding of structural and environmental effects on the interactions between carotenoids and tetrapyrroles and thereby better defining their role in controlling natural photosynthetic systems.     

Tetrapyrrole singlet excited state quenching by carotenoids in an artificial photosynthetic antenna

Two artificial photosynthetic antenna models consisting of a Si phthalocyanine (Pc) bearing two axially attached carotenoid moieties having either 9 or 10 conjugated double bonds are used to illustrate some of the function of carotenoids in photosynthetic membranes. Both models studied in toluene, methyltetrahydrofuran, and benzonitrile exhibited charge separated states of the type C*+-Pc*- confirming that the quenching of the Pc S1 state is due to photoinduced electron transfer. In hexane, the Pc S1 state of the 10 double bond carotenoid-Pc model was slightly quenched but the C*+-Pc*- transient was not spectroscopically detected. A semiclassical analysis of the data in hexane at temperatures ranging from 180 to 320 K was used to demonstrate that photoinduced electron transfer could occur. The model bearing the 10 double bond carotenoids exhibits biexponential fluorescence decay in toluene and in hexane, which is interpreted in terms of an equilibrium mixture of two isomers comprising s-cis and s-trans conformers of the carotenoid. The shorter fluorescence lifetime is associated with an s-cis carotenoid conformer where the close approach between the donor and acceptor moieties provides through-space electronic coupling in addition to the through-bond component.     

Electronic Structure and Triplet–Triplet Energy Transfer in Artificial Photosynthetic Antennas

Three Pd(II) phthalocyanine–carotenoid dyads featuring chromophores linked by amide bonds were prepared in order to investigate the rate of triplet–triplet (T‐T) energy transfer from the tetrapyrrole to the covalently attached carotenoid as a function of the number of conjugated double bonds in the carotenoid. Carotenoids having 9, 10 and 11 conjugated double bonds were studied. Transient absorption measurements show that intersystem crossing in the Pd(II) phthalocyanine takes place in 10 ps in each case and that T‐T energy transfer occurs in 126, 81 and 132 ps in the dyads bearing 9, 10 and 11 double bond carotenoids, respectively. To identify the origin of this variation in T‐T energy transfer rates, density functional theory (DFT) was used to calculate the T‐T electronic coupling in the three dyads. According to the calculations, the primary reason for the observed T‐T energy transfer trend is larger T‐T electronic coupling between the tetrapyrrole and the 10‐double bond carotenoid. A methyl group adjacent to the amide linker that connects the Pd(II) phthalocyanine and the carotenoid in the 9 and 11‐double bond carotenoids is absent in the 10‐double bond carotenoid, and this difference alters its electronic structure to increase the coupling.     

Photoinduced electron transfer in a hexaphenylbenzene-based self-assembled porphyrin-fullerene triad

A hexaphenylbenzene-based zinc porphyrin dyad forms a 1:1 complex with a fullerene bearing two pyridyl groups via coordination of the pyridyl nitrogens with the zinc atoms. The fullerene is symmetrically located between the two zinc porphyrins. The binding constant for the complex is 7.3 x 10(4) M(-1) in 1,2-difluorobenzene. Photoinduced electron transfer from a porphyrin first excited singlet state to the fullerene occurs with a time constant of 3 ps, and the resulting charge-separated state has a lifetime of 230 ps. This self-assembled construct should form a basis for the construction of more elaborate model photosynthetic antenna-reaction center systems.     

Photonic switching of photoinduced electron transfer in a dithienylethene-porphyrin-fullerene triad molecule

A dithienylethene (DTE)-porphyrin (P)-fullerene (C(60)) triad molecule in which intramolecular photoinduced electron transfer is controlled by the photochromic DTE moiety has been prepared. Irradiation of the molecule with visible light gives the open form of the dithienylethene (DTEo). Excitation of the porphyrin gives DTEo-(1)P-C(60), which undergoes photoinduced electron transfer with a time constant of 25 ps to generate DTEo-P(.+)-C(60)(.-). Irradiation with ultraviolet light produces the closed form of the dithienylethene (DTEc). Excitation of DTEc-P-C(60) yields DTEc-(1)P-C(60), whose porphyrin first excited singlet state is quenched in 2.3 ps by singlet-singlet energy transfer to DTEc, generating (1)DTEc-P-C(60) and precluding significant photoinduced electron transfer. Such highly reversible photonically controlled intramolecular photoinduced electron transfer may eventually be useful in the design of photonic or optoelectronic devices.     

Photoswitched singlet energy transfer in a porphyrin-spiropyran dyad.

A photochromic nitrospiropyran moiety (Sp) has been covalently linked to a zinc (PZn) and to a free-base (P(H2)) porphyrin. In the resulting dyads (P(Zn)-Sp(c) and P(H2)-Sp(c)), the porphyrin first excited singlet states are unperturbed by the closed form of the attached spiropyran. Excitation of the spiropyran moiety of either dyad in the near-UV region results in ring opening to a merocyanine form (P-Sp(o)) that absorbs at 600 nm. The open form re-closes thermally in 2-methyltetrahydrofuran with a time constant of 20 s, or following irradiation into the 600 nm band. Excitation of the zinc porphyrin moiety in the merocyanine form of the dyad yields 1PZn-Sp(o). The lifetime of the zinc porphyrin excited state is reduced from its usual value of 1.8 ns to 130 ps by singlet-singlet energy transfer to the merocyanine moiety to give PZn-1Sp(o). The quantum yield of energy transfer is 0.93. Quenching is also observed in the free base dyad, where 1P(H2)-Sp(o) and P(H2)-1Sp(o) exchange singlet excitation energy. This photoswitchable quenching phenomenon provides light-activated control of the porphyrin excited states, and consequently control of any subsequent energy or electron-transfer processes that might be initiated by these excited states in more complex molecular photonic or optoelectronic devices.     

Energy transfer, excited-state deactivation, and exciplex formation in artificial caroteno-phthalocyanine light-harvesting antennas

We present results from transient absorption spectroscopy on a series of artificial light-harvesting dyads made up of a zinc phthalocyanine (Pc) covalently linked to carotenoids with 9, 10, or 11 conjugated carbon-carbon double bonds, referred to as dyads 1, 2, and 3, respectively. We assessed the energy transfer and excited-state deactivation pathways following excitation of the strongly allowed carotenoid S2 state as a function of the conjugation length. The S2 state rapidly relaxes to the S* and S1 states. In all systems we detected a new pathway of energy deactivation within the carotenoid manifold in which the S* state acts as an intermediate state in the S2-->S1 internal conversion pathway on a sub-picosecond time scale. In dyad 3, a novel type of collective carotenoid-Pc electronic state is observed that may correspond to a carotenoid excited state(s)-Pc Q exciplex. The exciplex is only observed upon direct carotenoid excitation and is nonfluorescent. In dyad 1, two carotenoid singlet excited states, S2 and S1, contribute to singlet-singlet energy transfer to Pc, making the process very efficient (>90%) while for dyads 2 and 3 the S1 energy transfer channel is precluded and only S2 is capable of transferring energy to Pc. In the latter two systems, the lifetime of the first singlet excited state of Pc is dramatically shortened compared to the 9 double-bond dyad and model Pc, indicating that the carotenoid acts as a strong quencher of the phthalocyanine excited-state energy.