Radium,uranium and thorium concentrations in low specific activity scales and in waters of some oil and gas production plants

Low specific activity scales consisting of alkaline earth metal carbonates and sulfates are often present in some gaseous and liquid hydrocarbon plants; these scales contain a certain concentration of radium, uranium and thorium, which can cause a risk of -irradiation and of internal radiocontamination when they must be mechanically removed. That being stated,²³⁸U,²³²Th and²²⁶Ra were determined in scales, sludges and waters coming from different plants.²³⁸U and²³²Th concentrations were found very low; the isotopes²³⁸U and²³⁴U resulted in radioactive equilibrium, whilst²³²Th and²²⁸Th were not always in equilibrium.²²⁶Ra concentration was higher in scales and sludges than in waters.     

Probing the Conformational Stability of Two Different Copper Proteins: A Dynamic Fluorescence Study on Azurin and Ascorbate Oxidase

Tryptophan fluorescence is extremely useful to monitor structural conformational transitions in proteins. Denaturant-induced unfolding of azurin and ascorbate oxidase has been studied by dynamic fluorescence measurements in the frequency domain and the results have been interpreted in terms of continuous distribution of lifetimes. The data add new information on the unfolding mechanism that was previously analyzed by steady-state emission spectroscopy. In particular, the existence of multiple, parallel unfolding pathways may be envisaged and correlated, in both cases, to the two protein structures. The effect of metal depletion has been also characterized by fluorescence lifetime measurements. In the case of azurin, a monomeric protein, the data demonstrate that copper removal yields a totally different unfolding pathways with respect to the holo protein, indicating that metal ion plays a fundamental structural role in the wild type, native protein. In the case of ascorbate oxidase a dimer of 140 kDa, only minor effects have been detected by copper removal. However, the analysis of the fluorescence decay in presence of different amounts of guanidinium hydrochloride gives new important insights on the unfolding intermediates. In particular the data support the hypothesis of a partial exposure of an outer layer of dimer at intermediate denaturant concentration. This ability of dynamic fluorescence to pinpoint the presence of structural micro-heterogeneity in the unfolding pathways of proteins demonstrates the greater power of this technique compared to the most commonly used steady-state measurements.     

Properties of alkali metal atoms deposited on a MgO surface: a systematic experimental and theoretical study

The adsorption of small amounts of alkali metal atoms (Li, Na, K, Rb, and Cs) on the surface of MgO powders and thin films has been studied by means of EPR spectroscopy and DFT calculations. From a comparison of the measured and computed g values and hyperfine coupling constants (hfccs), a tentative assignment of the preferred adsorption sites is proposed. All atoms bind preferentially to surface oxide anions, but the location of these anions differs as a function of the deposition temperature and alkali metal. Lithium forms relatively strong bonds with MgO and can be stabilized at low temperatures on terrace sites. Potassium interacts very weakly with MgO and is stabilized only at specific sites, such as at reverse corners where it can interact simultaneously with three surface oxygen atoms (rubidium and cesium presumably behave in the same way). Sodium forms bonds of intermediate strength and could, in principle, populate more than a single site when deposited at room temperature. In all cases, large deviations of the hfccs from the gas-phase values are observed. These reductions in the hfccs are due to polarization effects and are not connected to ionization of the alkali metal, which would lead to the formation of an adsorbed cation and a trapped electron. In this respect, hydrogen atoms behave completely differently. Under similar conditions, they form (H(+))(e(-)) pairs. The reasons for this different behavior are discussed.     

Li atoms deposited on single crystalline MgO(0 0 1) surface. A combined experimental and theoretical study

In this Letter the interaction of Li atoms with terrace sites of MgO(0 0 1) surfaces is characterized by combining EPR spectroscopy with theoretical calculations. The Li atoms adsorbed to MgO terrace sites show a reduction of the isotropic hyperfine coupling constant by approximately 50% with respect to Li atoms in the gas phase. In combination with theoretical calculations it can be shown that this reduction of the hyperfine coupling constant is not due to a charge transfer between Li and MgO but can be understood by a polarization of the Li atoms which are essentially neutral.     

A Bayesian approach to vectorization of object boundaries from digital images and to geometrical uncertainty assessment

A new Bayesian approach is presented for extracting 2D object boundaries with measures of uncertainty. The boundaries are described by minimal closed sequences of segments and arcs, called mixed polygons. The sequence is minimal in the sense that it is able to describe all the geometrical properties of the boundary without being redundant. Based on geometrical measures evaluated on the object boundary model, a prior distribution is introduced in order to favor a mixed polygon with good geometrical properties, avoiding short sides, collinearity between segments, and so on. The estimation process is based on a two‐stage procedure that combines reversible‐jump MCMC (RJMCMC) and classic MCMC methods. The RJMCMC method is viewed as a model selection technique, and it is used to estimate the correct number of sides of the mixed polygon. The MCMC algorithm provides a sample of mixed polygons through which to evaluate the mixed polygon that best approximates the object boundary and its geometrical uncertainty. A convergence criterion for the RJMCMC method is provided. Copyright © 2011 John Wiley & Sons, Ltd.     

Fabry-Perot interferometers built by photonic crystal fiber pressurization during fusion splicing

We report on a microscopic Fabry-Perot interferometer whose cavity is a bubble trapped inside an optical fiber. The microcavity is formed by pressuring a photonic crystal fiber (PCF) with large voids during fusion splicing with a conventional single-mode fiber. The technique allows achieving high repeatability and full control over the cavity size and shape. It was found that the size of the PCF voids contributes to control the cavity size independently of the pressure in the PCF. Our devices exhibit a record fringe contrast of 30?dB (visibility of 0.999) due to the ellipsoidal cavity whose surfaces compensate for the diffraction of the reflected beam. The strain sensitivity of the interferometers is higher when the cavities are ellipsoidal than when they are spherical.