Displacement affinity chromatography of protein phosphatase one (PP1) complexes

Background  

Protein phosphatase one (PP1) is a ubiquitously expressed, highly conserved protein phosphatase that dephosphorylates target protein serine and threonine residues. PP1 is localized to its site of action by interacting with targeting or regulatory proteins, a majority of which contains a primary docking site referred to as the RVXF/W motif.     

Experimental value of G A/GV from a measurement of both P-odd correlations in free-neutron decay

A new experimental value of the fundamental weak-interaction parameter λ=G _A/G_V (?1.2686±0.0046) is obtained for the first time by an original method that consists in measuring both P-odd correlations in free-neutron decay.     

Chemo- and regioselective cyclohydrocarbonylation of alpha-keto alkynes catalyzed by a zwitterionic rhodium complex and triphenyl phosphite

alpha-Keto alkynes react with CO and H(2) in the presence of catalytic quantities of the zwitterionic rhodium complex (eta(6)-C(6)H(5)BPh(3))(-)Rh(+)(1,5-COD) and triphenyl phosphite affording either the 2-, 2(3H)-, or 2(5H)-furanones in 61-93% yields. The cyclohydrocarbonylation is readily accomplished using substrates containing alkyl, aryl, vinyl, and alkoxy groups at the acetylenic terminal, as well as a variety of primary, secondary, and tertiary alkyl, aryl, and heteroaryl groups connected to the ketone functionality. Structural and electronic properties present in the starting materials mediate the chemo- and regioselectivity of the reaction.     

Experimental study of quasi-elastic scattering of ultracold neutrons

Ultracold neutrons (UCN) are lost from traps if they are quasi-elastically scattered from the wall with an energy gain sufficient to exceed the Fermi potential for the wall. Possible mechanisms of a quasi-elastic energy transfer are, for instance, scattering from hydrogen diffusing in an impurity surface layer or on surface waves at a liquid wall. Using two different experimental methods at the UCN source of the Institut Laue-Langevin we have investigated both the energy-gain and the energy-loss side of quasi-elastic UCN scattering on Fomblin grease coated walls. For Fomblin oil and similar new types of oil we report up-scattering data as a function of temperature and energy transfer. These low-temperature oils may be used in an improved measurement of the neutron lifetime, which requires extremely low wall reflection losses. Received 13 March 2002 Published online 31 July 2002     

Estimation of scalar and tensor weak-interaction constants on the basis of recent neutron experimental data

The contributions of scalar and tensor interactions associated with right-handed neutrinos to the effective hadron-lepton Hamiltonian are estimated on the basis of modern measurements of the neutron lifetime and correlations in the beta decay of a free neutron.