Crystallization of Arthrobacter sp. strain 1C N-(1-D-carboxyethyl)-L-norvaline dehydrogenase and its complex with NAD+

The novel NAD+-linked opine dehydrogenase from a soil isolate Arthrobacter sp. strain 1C belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificites. Crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 ? resolution. X-ray precession photographs have established that the crystals belong to space group P21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 ? and a single subunit in the asymmetric unit. The elucidation of the three-dimensional structure of this enzyme will provide a structural framework for this novel class of dehydrogenases to enable a comparison to be made with other enzyme families and also as the basis for mutagenesis experiments directed towards the production of natural and synthetic opine-type compounds containing two chiral centres.     

关于极端原子核的相对论自洽角动量投影壳模型计算(英文)

相对论自洽角动量投影壳模型是最近发展出来的一个自洽模型, 它对于不同核区具有稳定的参数, 能够很好地描述已知和未知具有稳定形变的各种原子核的性质。 计算了若干包括稳定核素、 极端核素和超重核素, 并把计算结果与现有的实验数据进行了比较。     

Mixed Symmetry States in Even—Even ^96—108Mo Nuclei

Excitation energies and electromagnetic transition strengths in even-even ^96-108Mo nuclei have been described systematically be using the proton-neutron interacting boson model (IBM-2).It appears that the properties of low-lying levels in these isotopes,for which the comparison between experiment and theory is possible,can be satisfactorily described by the IBM-2 model,provided proper account is taken of the presence at low energy of states having a mixed-symmetry character.It seems possible to identify,in each isotope,a few states having such a character,the lowest ones being either 22^ or 23^ levels.It is found that these nuclei are in the transition from U(5) to SU(3).     

Integral resource capacity planning for inpatient care services based on bed census predictions by hour

The design and operations of inpatient care facilities are typically largely historically shaped. A better match with the changing environment is often possible, and even inevitable due to the pressure on hospital budgets. Effectively organizing inpatient care requires simultaneous consideration of several interrelated planning issues. Also, coordination with upstream departments like the operating theatre and the emergency department is much-needed. We present a generic analytical approach to predict bed census on nursing wards by hour, as a function of the Master Surgical Schedule and arrival patterns of emergency patients. Along these predictions, insight is gained on the impact of strategic (ie, case mix, care unit size, care unit partitioning), tactical (ie, allocation of operating room time, misplacement rules), and operational decisions (ie, time of admission/discharge). The method is used in the Academic Medical Center Amsterdam as a decision-support tool in a complete redesign of the inpatient care operations.